HPLC Column Cleaning And Regeneration

If adequate care is taken, it is possible to maintain column efficiency and reliability over an extended period of time. This document is intended to give information on the different procedures to help extend SiliaChrom® HPLC columns lifetime. This document will also cover column storage.

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  1. SiliaChrom® Semi-Preparative HPLC Columns XDB Si

    SiliaChrom® Semi-Preparative HPLC Columns XDB Si

    The pore size of this silica support is intermediate, 150 Å, which separates larger molecules than its 100 Å equivalent. The lower surface area of this phase allows compounds to have shorter retention times on the column.

  2. SiliaChrom® Preparative HPLC Columns XDB Si (H110005H-Y250)

    SiliaChrom® Preparative HPLC Columns XDB Si (H110005H-Y250)

    The pore size of this silica support is intermediate, 150 Å, which separates larger molecules than its 100 Å equivalent. The lower surface area of this phase allows compounds to have shorter retention times on the column.

  3. SiliaChrom® Semi-Preparative HPLC Columns XDB C18

    SiliaChrom® Semi-Preparative HPLC Columns XDB C18

    SiliaChrom® XDB C18 presents the same bonded phase as the SiliaChrom XDB 1 C18 and XDB1 C18-300. The pore size of the silica support is intermediate, 150 Å, which allows to separate larger molecules than its 100 Å equivalent. The lower surface associated allows compounds to have shorter retention times. SiliaChrom XDB C18 is an excellent equivalent to Hypersil BDS-C18 and Zorbax XDB-C18.

  4. SiliaChrom® Preparative HPLC Columns XDB C18 (H111805H-Y250)

    SiliaChrom® Preparative HPLC Columns XDB C18 (H111805H-Y250)

    SiliaChrom® XDB C18 presents the same bonded phase as the SiliaChrom XDB 1 C18 and XDB1 C18-300. The pore size of the silica support is intermediate, 150 Å, which allows to separate larger molecules than its 100 Å equivalent. The lower surface associated allows compounds to have shorter retention times. SiliaChrom XDB C18 is an excellent equivalent to Hypersil BDS-C18 and Zorbax XDB-C18.

  5. SiliaChrom® Semi-Preparative HPLC Columns XDB C8

    SiliaChrom® Semi-Preparative HPLC Columns XDB C8

    SiliaChrom® XDB C8 presents the same bonded phase as the SiliaChrom XDB 1 C18 and XDB1 C18-300. The pore size of the silica support is intermediate, 150 Å, which allows to separate larger molecules than its 100 Å equivalent. The lower surface associated allows compounds to have shorter retention times. SiliaChrom XDB C18 is an excellent equivalent to Hypersil BDS-C18 and Zorbax XDB-C18.

  6. SiliaChrom® Preparative HPLC Columns XDB C8 (H110805H-Y250)

    SiliaChrom® Preparative HPLC Columns XDB C8 (H110805H-Y250)

    SiliaChrom® XDB C8 presents the same bonded phase as the SiliaChrom XDB 1 C18 and XDB1 C18-300. The pore size of the silica support is intermediate, 150 Å, which allows to separate larger molecules than its 100 Å equivalent. The lower surface associated allows compounds to have shorter retention times. SiliaChrom XDB C18 is an excellent equivalent to Hypersil BDS-C18 and Zorbax XDB-C18.

  7. SiliaChrom® Preparative HPLC Columns XT C18

    SiliaChrom® Preparative HPLC Columns XT C18

    SiliaChrom® XT C18 has a hybrid surface made from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, presents a very high resistance to extreme high pH conditions (up to 12).

  8. SiliaChrom® Preparative HPLC Columns HILIC

    SiliaChrom® Preparative HPLC Columns HILIC

    SiliaChrom® HILIC (Hydrophilic Interaction Chromatography) HPLC columns are designed to retain highly polar analytes. SiliaChrom HILIC has a selectivity that is complementary to the one of reversed-phase columns. In fact, it has a higher retention for hydrophilic compounds in HILIC mode. HILIC sorbent is more stable and reproducible than normal phase silica or amino columns. This phase is ideal for MedChem laboratories and approved for SFC applications.

  9. SiliaChrom® Preparative HPLC Columns AQ C8

    SiliaChrom® Preparative HPLC Columns AQ C8

    SiliaChrom® AQ C8 columns use a new generation adsorbent based on high purity spherical silica and unique bonding technology.

  10. SiliaChrom® Preparative HPLC Columns AQ C18

    SiliaChrom® Preparative HPLC Columns AQ C18

    SiliaChrom® AQ C18 columns use a new generation adsorbent based on high purity spherical silica and unique bonding technology. 

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