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Solid Phase Extraction (SPE)

Solid-phase extraction (SPE) is a technique designed for rapid sample preparation and purification prior chromatographic analysis. You can optimize your SPE protocols by using SiliCycle SiliaPrep™ and SiliaPrepX™ SPE Cartridges and Well Plates.

For your convenience, SiliaPrep and SiliaPrepX are available in different formats; cartridges (SiliaPrep SPE Cartridges), and well plates (SiliaPrep 96 Well Plates) with various sorbents (SiliaFlash® andSiliaBond®), and bed weight (up to 20 grams). The well plates are used in high throughput combinatorial chemistry, drug discovery and screening, and metabolism pharmacokinetics applications and for automated method as multiprobe approach.

By using SiliaPrep and SiliaPrepX products you will generate higher purity samples and reduce the number of false positives in your screening, resulting in higher quality data. All the SiliaPrep are packed with sorbents based on the fines-free SiliaFlash silica gel.

APPLICATION NOTES

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  1. SiliaPrep™ SPE Cartridges Silica

    SiliaPrep™ SPE Cartridges Silica

    SiliaPrep Silica is the most polar sorbent presenting a slightly acidic character used to extract various compounds from non-polar solvents using hydrogen bonding. Efficient sorbent for the separation of analytes with similar structures and for removing the baseline noise from organic samples.

  2. SiliaPrep™ SPE Cartridges Amine (WAX)

    SiliaPrep™ SPE Cartridges Amine (WAX)

    SiliaPrep Amine is a weak anion exchanger sorbent used in replacement of SAX for strong anions to avoid irreversible retention. Can also be employed as a metal scavenger and norman phase conditions.

  3. SiliaPrep™ SPE Cartridges Amine nec (WAX)

    SiliaPrep™ SPE Cartridges Amine nec (WAX)

    SiliaPrep Amine nec is a weak anion exchanger sorbent used in replacement of SAX for strong anions to avoid irreversible retention. Can also be employed as a metal scavenger and norman phase conditions.

  4. SiliaPrep™ SPE Cartridges Carboxylic Acid (WCX)

    SiliaPrep™ SPE Cartridges Carboxylic Acid (WCX)

    SiliaPrep Carboxilic Acid is a weak cation exchanger sorbent (WCX) used to extract strong basics compounds.

  5. SiliaPrep™ SPE Cartridges Carboxylic Acid nec (WCX)

    SiliaPrep™ SPE Cartridges Carboxylic Acid nec (WCX)

    SiliaPrep Carboxilic Acid nec is a weak cation exchanger sorbent (WCX) used to extract strong basics compounds.

  6. SiliaPrep™ SPE Cartridges Cyano

    SiliaPrep™ SPE Cartridges Cyano

    SiliaPrep Cyano is a moderate polar sorbent used as reversed-phase (less hydrophobic than C18 and C8 phases) and normal phase (less polar compared to silica) to extract acidic, neutral, basic compounds from aqueous solutions.

  7. SiliaPrep™ SPE Cartridges Cyano nec

    SiliaPrep™ SPE Cartridges Cyano nec

    SiliaPrep Cyano nec is a moderate polar sorbent used as reversed-phase (less hydrophobic than C18 and C8 phases) and normal phase (less polar compared to silica) to ectract acidic, neutral, basic compounds from aqueous solutions.

  8. SiliaPrep™ SPE Cartridges Diol

    SiliaPrep™ SPE Cartridges Diol

    SiliaPrep Diol nec is a moderate polar sorbent presenting neutral character used to extract polar compounds from non-polar solvents, structural isomers. Alternative to silica when the acidic character is problematic.

  9. SiliaPrep™ SPE Cartridges C1

    SiliaPrep™ SPE Cartridges C1

    SiliaPrep C1 is a non-polar sorbent and presents a mederate hydrophobicity. This phase is used to extract extremely non-polar compounds.

  10. SiliaPrep™ SPE Cartridges C2

    SiliaPrep™ SPE Cartridges C2

    SiliaPrep C2 is a non-polar sorbent and presents a moderate hydrophobicity. This phase is used to extract extremely non-polar compounds.

  11. SiliaPrep™ SPE Cartridges C2 nec

    SiliaPrep™ SPE Cartridges C2 nec

    SiliaPrep C2 nec is a non-polar sorbent and presents a moderate hydrophobicity. This phase is used to extract extremely non-polar compounds.

  12. SiliaPrep™ SPE Cartridges C4

    SiliaPrep™ SPE Cartridges C4

    SiliaPrep C4 is a non-polar sorbent and presents a moderate hydrophobicity. This phase is used to extract extremely non-polar compounds.

  13. SiliaPrep™ SPE Cartridges C4 nec

    SiliaPrep™ SPE Cartridges C4 nec

    SiliaPrep C8 nec is a non-polar sorbent and presents a moderate hydrophobicity. This phase is used to extract extremely non-polar compounds.

  14. SiliaPrep™ SPE Cartridges C8

    SiliaPrep™ SPE Cartridges C8

    SiliaPrep C8 nec is a non-polar sorbent and presents a moderate hydrophobicity. This phase is used to extract extremely non-polar compounds. The SiliaPrep C8 nec is more selective than C18 phase for compounds such as heavy PAH, vitamins D and oil & greasy compounds.

  15. SiliaPrep™ SPE Cartridges C8 nec

    SiliaPrep™ SPE Cartridges C8 nec

    SiliaPrep C8 nec is a non-polar sorbent and presents a moderate hydrophobicity. This phase is used to extract extremely non-polar compounds. The SiliaPrep C8 nec is more selective than C18 phase for compounds such as heavy PAH, vitamins D and oil & greasy compounds.

  16. SiliaPrep™ SPE Cartridges C18 nec (23%)

    SiliaPrep™ SPE Cartridges C18 nec (23%)

    SiliaPrep C18 is a non-polar sorbent used to extract acidic, neutral and acidic compounds from aqueous solutions, various organic compounds from water as well as drugs & metabolites from physiological fluids and various compounds from water, foods & beverages. Presence of free silanols allows secondary interactions for the extraction of basic compounds.

  17. SiliaPrep™ SPE Cartridges C18 (17%)

    SiliaPrep™ SPE Cartridges C18 (17%)

    SiliaPrep C18 is a non-polar sorbent used to extract acidic, neutral and acidic compounds from aqueous solutions, various organic compounds from water as well as drugs & metabolites from physiological fluids.

  18. SiliaPrep™ SPE Cartridges C18 nec (17%)

    SiliaPrep™ SPE Cartridges C18 nec (17%)

    SiliaPrep C18 nec is a non-polar sorbent similar to SiliaPrep C18 but presents higher retention and polar selectivity for basic compounds. Unreacted surface OH's can be used for soft condition of catch and release purification of glucoronides for example.

  19. SiliaPrep™ SPE Cartridges C18 (11%)

    SiliaPrep™ SPE Cartridges C18 (11%)

    SiliaPrep C18 (11%) is a non-polar sorbent used to elute very large hydrophobic molecules that are too strongly retained on higher carbon loading.

  20. SiliaPrep™ SPE Cartridges C18 nec (11%)

    SiliaPrep™ SPE Cartridges C18 nec (11%)

    SiliaPrep C18 (11%) is a non-polar sorbent used to elute very large hydrophobic molecules that are too strongly retained on higher carbon loading. Use this less retentive phase for a rapid release of hydrophobic compounds using weaker organic solvents at lower volumes.

  21. SiliaPrep™ SPE Cartridges Cyclohexyl

    SiliaPrep™ SPE Cartridges Cyclohexyl

    SiliaPrep Cyclohexyl is a non-polar sorbent used to extract compounds from aqueous solution and presents a different selectivity compared to the C8 and C18 phases.

  22. SiliaPrep™ SPE Cartridges Phenyl

    SiliaPrep™ SPE Cartridges Phenyl

    SiliaPrep Phenyl is a non-polar sorbent used to extract compounds from aqueous solution and presents high selectivity for aromatics compounds compared to the other non-polar phases caused by the pi-pi interactions.

  23. SiliaPrep™ SPE Cartridges Piperazine (SPE-R60030B)

    SiliaPrep™ SPE Cartridges Piperazine (SPE-R60030B)

    SiliaPrep Piperazine is a weak anion exchanger that can be used in replacement of SAX and SAX-2 for strong anions to avoid irreversible retention. By using this phase, elution can be done at a lower pH (around pH 8-9) comparatively to the SiliaPrep Amine.

  24. SiliaPrep™ SPE Cartridges Diethylamine (WAX-2)

    SiliaPrep™ SPE Cartridges Diethylamine (WAX-2)

    SiliaPrep Diethylamine (WAX-2) is a weak anion exchanger that can be used in replacement of SAX and SAX-2 for strong anions to avoid irreversible retention. This phase presents a slightly lower capacity and a moderate non polar character due to the alkyl chain on the amino function. Moreover, it can be employed as metal scavenger and in normal phase conditions.

  25. SiliaPrep™ SPE Cartridges Pentafluoprophenyl

    SiliaPrep™ SPE Cartridges Pentafluoprophenyl

    SiliaPrep Pentafluorophenyl is a fluorous sorbent used to separate fluorinated molecules and non-fluorinated ones. The electron density presents fluorinated aromatic ring which affords an enhancement in the retention of conjugated and aromatic ring-containing due to the desirable pi-pi interactions. Excellent alternative of the C18 when the C18 presents a selectivity problem.

  26. SiliaPrep™ SPE Cartridges Fluorochrom

    SiliaPrep™ SPE Cartridges Fluorochrom

    SiliaPrep Fluorochrom is a fluorous sorbent used to separate fluorinated molecules and non-fluorinated ones.

  27. SiliaPrep™ SPE Cartridges Tridecafluoro

    SiliaPrep™ SPE Cartridges Tridecafluoro

    SiliaPrep Tridecafluoro is a fluorous sorbent used to separate fluorinated molecules and non-fluorinated ones.

  28. SiliaPrep™ SPE Cartridges TMA Chloride nec (SAX)

    SiliaPrep™ SPE Cartridges TMA Chloride nec (SAX)

    SiliaPrep TMA Chloride (SAX) is a strong anion exchanger sorbent positively charged under all conditions used to extract acid molecules (pKa 3-5).

  29. SiliaPrep™ SPE Cartridges TMA Acetate nec

    SiliaPrep™ SPE Cartridges TMA Acetate nec

    SiliaPrep TMA Acetate (SAX-2) is a strong anion exchanger sorbent with a low selectivity acetate counter ion already in place. This sorbent retains more favorably acidic compounds with pKas <5, such as carboxylic acid.

  30. SiliaPrep™ SPE Cartridges Tosic Acid (SCX)

    SiliaPrep™ SPE Cartridges Tosic Acid (SCX)

    SiliaPrep Tosic Acid (SCX) is a strong cation exchanger and maintains a negative charge throughtout the pH scale. The most common use is a strong cation exchanger for the amine and basics molecules "catch and release" purification technique in SPE cartridges.

  31. SiliaPrep™ SPE Cartridges Tosic Acid nec (SCX)

    SiliaPrep™ SPE Cartridges Tosic Acid nec (SCX)

    SiliaPrep Tosic Acid is a strong cation exchanger and maintains a negative charge throughtout the pH scale. The most common use is a strong cation exchanger for the amine and basics molecules "catch and release" purification technique in SPE cartridges.

  32. SiliaPrep™ SPE Cartridges Propylsulfonic Acid (SCX-2)

    SiliaPrep™ SPE Cartridges Propylsulfonic Acid (SCX-2)

    SiliaPrep Propylsulfonic Acid (SCX-2) is a strong cation exchanger and maintains a negative charge throughtout the pH scale. The most common use is a strong cation exchanger for the amine and basics molecules "catch and release" purification technique in SPE cartridges.

  33. SiliaPrep™ SPE Cartridges Propylsulfonic Acid nec (SCX-2)

    SiliaPrep™ SPE Cartridges Propylsulfonic Acid nec (SCX-2)

    SiliaPrep Propylsulfonic Acid nec is a strong cation exchanger and maintains a negative charge throughtout the pH scale. The most common use is a strong cation exchanger for the amine and basics molecules "catch and release" purification technique in SPE cartridges.

  34. SiliaPrep™ SPE Cartridges Florisil

    SiliaPrep™ SPE Cartridges Florisil

    SiliaPrep Florisil is a polar sorbent presenting basic character used to extract non-polar to moderate polar compounds from non-polar solvents. This sorbent contain magnesium ion which allows retention for chlorinated pesticides, polychlorinated biphenyl and polysaccharides.

  35. SiliaPrep™ SPE Cartridges Florisil - PR Grade

    SiliaPrep™ SPE Cartridges Florisil - PR Grade

    SiliaPrep Florisil is a polar sorbent presenting basic character used to extract non-polar to moderate polar compounds from non-polar solvents. This sorbent contain magnesium ion which allows retention for chlorinated pesticides.

  36. SiliaPrep™ SPE Cartridges Alumina Acidic

    SiliaPrep™ SPE Cartridges Alumina Acidic

    SiliaPrep Alumina Acidic is a polar sorbent basic character used to extract compounds as described for SiliaPrep Silica. SiliaPrep Alumina Acidic is more stable at high pH than silica.

  37. SiliaPrep™ SPE Cartridges Alumina Neutral

    SiliaPrep™ SPE Cartridges Alumina Neutral

    SiliaPrep Alumina Neutral is a polar sorbent basic character used to extract compounds as described for SiliaPrep Silica. SiliaPrep Alumina Neutral is more stable at high pH than silica.

  38. SiliaPrep™ SPE Cartridges Alumina Basic

    SiliaPrep™ SPE Cartridges Alumina Basic

    SiliaPrep Alumina Basic is a polar sorbent basic character used to extract compounds as described for SiliaPrep Silica. SiliaPrep Alumina Basic is more stable at high pH than silica.

  39. SiliaPrep™ SPE Cartridges C8/SAX

    SiliaPrep™ SPE Cartridges C8/SAX

    SiliaPrep C8/SAX is a mixed-mode used to extract acidic compounds from aqueous solutions, typically drugs and metabolites from physiological fluids.

  40. SiliaPrep™ SPE Cartridges C8/TMA ACETATE nec

    SiliaPrep™ SPE Cartridges C8/TMA ACETATE nec

    SiliaPrep C8/SAX-2 is a mixed-mode used to extract acidic compounds from aqueous solutions, typically drugs and metabolites from physiological fluids.

  41. SiliaPrep™ SPE Cartridges C8/SCX-2

    SiliaPrep™ SPE Cartridges C8/SCX-2

    SiliaPrep C8/SCX-2 is a mixed-mode sorbent used to extract basic compounds from aqueous solutions, typically drugs and metabolites from physiological fluids.

  42. SiliaPrep™ SPE Cartridges SCX-2/SAX

    SiliaPrep™ SPE Cartridges SCX-2/SAX

    SiliaPrep SCX-2/SAX is a mixed-mode used to separate acidic and basic molecules from non-ionisable molecules.

  43. SiliaPrep™ SPE Cartridges C8/SCX-2/SAX

    SiliaPrep™ SPE Cartridges C8/SCX-2/SAX

    SiliaPrep C8/SCX-2/SAX is a tri-mode extraction and fractionation of acidic, neutral and basic compounds from aqueous solutions, typically drugs and metabolites from physiological fluids.

  44. SiliaPrep™ SPE Cartridges C8/SCX-2/TMA ACETATE nec

    SiliaPrep™ SPE Cartridges C8/SCX-2/TMA ACETATE nec

    SiliaPrep C8/SCX-2/SAX-2 is a tri-mode extraction and fractionation of acidic, neutral and basic compounds from aqueous solutions, typically drugs and metabolites from physiological fluids.

  45. SiliaPrep™ SPE Cartridges C8/SCX

    SiliaPrep™ SPE Cartridges C8/SCX

    SiliaPrep C8/SCX is a mixed-mode sorbent used to extract basic compounds from aqueous solutions, typically drugs and metabolites from physiological fluids.

  46. SiliaPrep™ SPE Cartridges C18/TMA ACETATE nec

    SiliaPrep™ SPE Cartridges C18/TMA ACETATE nec

    SiliaPrep C18/SAX-2 is a mixed-mode sorbent used to extract acidic compounds from aqueous solutions, typically drugs and metabolites from physiological fluids.

  47. SiliaPrep™ SPE Cartridges C18/SAX

    SiliaPrep™ SPE Cartridges C18/SAX

    SiliaPrep C18/SAX is a mixed-mode sorbent used to extract acidic compounds from aqueous solutions, typically drugs and metabolites from physiological fluids.

  48. SiliaPrep™ SPE Cartridges C18/SCX

    SiliaPrep™ SPE Cartridges C18/SCX

    SiliaPrep C18/SCX is a mixed-mode sorbent used as an alternative to the SiliaPrep C8/SCX & C8/SCX-2 for the extraction of basic compounds requiring more non-polar retention.

  49. SiliaPrep™ SPE Cartridges C18/SCX-2

    SiliaPrep™ SPE Cartridges C18/SCX-2

    SiliaPrep C18/SCX-2 is a mixed-mode sorbent used as an alternative to the SiliaPrep C8/SCX & C8/SCX-2 for the extraction of basic compounds requiring more non-polar retention.

  50. SiliaPrep™ SPE Cartridges C18/WCX

    SiliaPrep™ SPE Cartridges C18/WCX

    SiliaPrep C18/WCX is a mixed-mode sorbent used to extract basic compounds from aqueous solutions, typically strong bases and quaternary amines.

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Support Backbone
  1. Polymeric Resins (12)
Application Fields
  1. Bio-Pharma (6)
  2. Energy (2)
  3. Environment (3)
  4. Food & Beverage (5)
  5. Forensic (17)
  6. Pharmaceutical (3)
Type of Column
  1. Narrow Bore Columns (2)
  2. Analytical Columns (4)
  3. Semi-Preparative Columns (1)
  4. Preparative Columns (1)
Chromatographic Mode
  1. Reversed Phases (6)
USP Code
  1. L1 (4)
Metal to Remove
Cadmium (Cd)
  1. Preferred Scavengers (2)
  2. Scavenge (3)
Cobalt (Co)
  1. Scavenge (6)
Chromium (Cr)
  1. Preferred Scavengers (5)
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  1. Scavenge (6)
Iron (Fe)
  1. Scavenge (6)
Mercury (Hg)
  1. Scavenge (4)
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  1. Preferred Scavengers (1)
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  1. Preferred Scavengers (1)
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Palladium (Pd-2+)
  1. Preferred Scavengers (4)
  2. Scavenge (2)
Palladium (Pd-0)
  1. Preferred Scavengers (4)
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  1. Preferred Scavengers (6)
Rhodium (Rh-1+)
  1. Preferred Scavengers (3)
Rhodium (Rh-2+)
  1. Preferred Scavengers (3)
Rhodium (Rh-3+)
  1. Preferred Scavengers (1)
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  1. Preferred Scavengers (1)
  2. Scavenge (3)
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  1. Scavenge (1)
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  1. Scavenge (5)
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  1. Scavenge (6)
Family / Formats
  1. SiliaChrom® (HPLC Columns) (6)
  2. SiliaPrep™ (SPE Cartridges) (98)
  3. SiliaPrepX™ (SPE Polymeric Cartridges) (12)
Phases
  1. Activated Carbon (1)
  2. Activated Carbon/Amine (WAX) (1)
  3. Activated Carbon/Diamine (PSA) (1)
  4. Alumina (3)
  5. Amine (4)
  6. C1 (1)
  7. C18 (18)
  8. C2 (3)
  9. C4 (3)
  10. C8 (14)
  11. Carbonate (2)
  12. Carboxylic Acid (WCX) (4)
  13. CleanDRUG (1)
  14. CleanENVI (1)
  15. Cyano (4)
  16. Cyclohexyl (1)
  17. Diamine (2)
  18. Diethylamine (WAX-2) (2)
  19. Dimercaptotriazine (DMT) (1)
  20. Diol (2)
  21. dt C18 (3)
  22. DVB (2)
  23. Florisil (2)
  24. Fluorochrom (2)
  25. HLB (2)
  26. Pentafluorophenyl (2)
  27. Phenyl (2)
  28. Piperazine (1)
  29. Propylsulfonic Acid (SCX-2) (4)
  30. SAX (10)
  31. SCX (6)
  32. SCX-2 (7)
  33. Silica (2)
  34. TMA Acetate (5)
  35. TMA Chloride (SAX) (2)
  36. Tosic Acid (SCX) (4)
  37. Triamine (1)
  38. Tridecafluoro (2)
  39. WAX (2)
  40. WCX (4)
  41. XT C18 (3)
Pore Size
  1. 60 Å (104)
  2. 100 Å (3)
  3. 110 Å (3)
  4. 150 Å (3)
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  1. Endcapped (115)
  2. Non-endcapped (14)
Cartridges Volume
  1. 1 mL (63)
  2. 2 mL (42)
  3. 3 mL (67)
  4. 6 mL (67)
  5. 12 mL (57)
  6. 25 mL (57)
Bed Weight
  1. 10 mg (6)
  2. 30 mg (12)
  3. 50 mg (92)
  4. 60 mg (6)
  5. 100 mg (99)
  6. 200 mg (64)
  7. 250 mg (3)
  8. 500 mg (67)
  9. 1 g (58)
  10. 2 g (58)
  11. 5 g (57)
Internal Diameter
  1. 2.1 mm (2)
  2. 3.0 mm (2)
  3. 4.6 mm (4)
  4. 10 mm (1)
  5. 20 mm (1)
Length
  1. 10 mm (2)
  2. 20 mm (3)
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  4. 50 mm (4)
  5. 100 mm (4)
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  7. 200 mm (2)
  8. 250 mm (2)
Particle Size
  1. 2.5 µm (2)
  2. 3 µm (6)
  3. 5 µm (6)
  4. 10 µm (2)
  5. 40 µm (2)
  6. 85 µm (10)
  7. 40-63 µm / 230-400 mesh (94)

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