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SPE Method of Caffeine, Cotinine and Nicotine Quantification Using SiliaPrepX™ HLB

SiliCycle has developed the SiliaPrepX™ HLB, an advanced hydrophilic and lipophilic polymer sorbent, for specific solid phase extraction of acidic, basic and neutral analytes from biological fluids and aqueous samples. The unique hydrophilic and lipophilic backbone allows an excellent mass transfer ofthe analytes into the polymeric matrix.

The performance of the SiliaPrepX HLB polymeric SPE cartridges in the treatment of caffeine, cotinine and nicotine in urine is presented following the link below. The comparison with competitive products at 2 levels of concentration, ion suppression measured and lot-to-lot reproducibility are also evaluated.

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  1. SiliaChrom® Narrow Bore HPLC Columns XT C18

    SiliaChrom® Narrow Bore HPLC Columns XT C18

    SiliaChrom® XT C18 has a hybrid surface made from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, presents a very high resistance to extreme high pH conditions (up to 12).

  2. SiliaChrom® Analytical Guard Cartridge XT C18

    SiliaChrom® Analytical Guard Cartridge XT C18

    The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.

  3. SiliaChrom® Analytical HPLC Columns XT C18

    SiliaChrom® Analytical HPLC Columns XT C18

    SiliaChrom® XT C18 has a hybrid surface made from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, presents a very high resistance to extreme high pH conditions (up to 12).

  4. SiliaPrepX™ HLB Polymeric SPE Cartridges

    SiliaPrepX™ HLB Polymeric SPE Cartridges

    The SiliaPrepX™ HLB is a wettable copolymer presenting a Hydrophobic-Lipophilic Balance (HLB)  permitting a strong retention for neutral, acidic and basic compounds and a high stability in organic silvents.

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