Quick Overview Of Column Chromatography
Before performing a separation on a chromatographic column, it is highly recommended to make different tests on thin layer chromatography (TLC) to find the best conditions for the separation. It is then important to use the same solvent system and adsorbent afterwards, on flash chromatography.
This document includes the principal common steps for performing flash chromatography:
1-Methodology
2-Theory of separation
3-Choice of solvent
4-Loading the column
5-Solvent equilibration
6-Loading of the sample
7-Column capacity
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SiliaBond® C18 (23%)
SiliaBond® C18 (or C18) is the traditional matrix for reversed-phase chromatography. The high loading provides the highest degree of hydrophobicity commercially available. Considered as the least selective phase since it interacts with a wide range of organic molecules.
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SiliaBond® C18 nec (23%)
SiliaBond® C18 (or C18) is the traditional matrix for reversed-phase chromatography. The high loading provides the highest degree of hydrophobicity commercially available. Considered as the least selective phase since it interacts with a wide range of organic molecules. Also used in ion-pairing chromatography (non-endcapped) for the separation of biomolecules.
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SiliaBond® C18 (17%)
The SiliaBond® C18 17% BDS is the most efficient deactivated C18 reversed-phase. This versatile support is the best choice for your applications in reversed-phase chromatography. This chromatographic phase has been compared with different competitors and presents a better separation for basic compounds.
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SiliaBond® C18 nec (17%)
This non-endcapped version of the SiliaBond® C18 17% BDS, presents a perfect monolayer of dimethyloctadecylsilane and silanol functions. The hydrophilic/hydrophobic balance is permitting to use a mobile phase with a higher concentration of water and ion pairing conditions for separation of biomolecules.
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SiliaBond® C8
SiliaBond® C8 (or C8) is a reversed-phase matrix with a moderate degree of hydrophobicity that works well for separating a wide range of compounds. It can be used instead of C18 when shorter retention times are desired.
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SiliaBond® C8 nec
SiliaBond® C8 (or C8) is a reversed-phase matrix with a moderate degree of hydrophobicity that works well for separating a wide range of compounds. It can be used instead of C18 when shorter retention times are desired.
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SiliaBond® C4
SiliaBond® C4 (or C4) provides less retention towards non-polar compounds than C18 and C8, and is useful in ion-pairing chromatography or to separate large biomolecules. It works best for molecules with a large hydrophilic region or in case where the hydrophobic region is buried within the structure.
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SiliaBond® C4 nec
SiliaBond® C4 (or C4) provides less retention towards non-polar compounds than C18 and C8, and is useful in ion-pairing chromatography or to separate large biomolecules. It works best for molecules with a large hydrophilic region or in case where the hydrophobic region is buried within the structure.
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SiliaBond® C2
SiliaBond® C2 nec is a non-polar sorbent and presents a moderate hydrophobicity. This phase is used to extract extremely non-polar compounds.
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SiliaBond® C2 nec
SiliaBond® C2 nec is a non-polar sorbent and presents a moderate hydrophobicity. This phase is used to extract extremely non-polar compounds.
- Metal to Remove
- Family / Formats
- Phases
- Pore Size
- Endcapping
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- Endcapped (44)
- Non-endcapped (9)
- Particle Size
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- 0-20 µm (1)
- 10-30 µm (1)
- 5-20 µm (1)
- 15-25 µm (2)
- 15-35 µm (2)
- 15-40 µm (1)
- 20-45 µm (2)
- 40-63 µm (23)
- 40-75 µm (1)
- 60-120 µm (2)
- 60-200 µm (4)
- 75-200 µm (1)
- 120-200 µm (2)
- 200-500 µm (4)
- 250-500 µm (1)
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