Quick Overview Of Column Chromatography
Before performing a separation on a chromatographic column, it is highly recommended to make different tests on thin layer chromatography (TLC) to find the best conditions for the separation. It is then important to use the same solvent system and adsorbent afterwards, on flash chromatography.
This document includes the principal common steps for performing flash chromatography:
1-Methodology
2-Theory of separation
3-Choice of solvent
4-Loading the column
5-Solvent equilibration
6-Loading of the sample
7-Column capacity
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SiliaBond® C18 nec (23%)
SiliaBond® C18 (or C18) is the traditional matrix for reversed-phase chromatography. The high loading provides the highest degree of hydrophobicity commercially available. Considered as the least selective phase since it interacts with a wide range of organic molecules. Also used in ion-pairing chromatography (non-endcapped) for the separation of biomolecules.
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SiliaBond® C18 nec (17%)
This non-endcapped version of the SiliaBond® C18 17% BDS, presents a perfect monolayer of dimethyloctadecylsilane and silanol functions. The hydrophilic/hydrophobic balance is permitting to use a mobile phase with a higher concentration of water and ion pairing conditions for separation of biomolecules.
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SiliaBond® Diol
SiliaBond Diol may also be used as polar sorbent in normal phase and aqueous size exclustion chromatography. Like bare silica, SiliaBond Diol has the ability to form hydrogen bonds and the capacity to separate structural isomers. Since most of its surface is covered with organic functions, the SiliaBond Diol absorbs less water, which leads to a more reproducible activity. It is also the sorbent of choice when working in normal phase in the presence of water.
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- Family / Formats
- Phases
- Pore Size
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- 60 Å (3)
- Particle Size
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