Quick Overview Of Column Chromatography
Before performing a separation on a chromatographic column, it is highly recommended to make different tests on thin layer chromatography (TLC) to find the best conditions for the separation. It is then important to use the same solvent system and adsorbent afterwards, on flash chromatography.
This document includes the principal common steps for performing flash chromatography:
1-Methodology
2-Theory of separation
3-Choice of solvent
4-Loading the column
5-Solvent equilibration
6-Loading of the sample
7-Column capacity
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SiliaSphere™ Spherical Silica Gels Preparative Chromatography PC Silica
SiliCycle offers a wide selection of spherical silica gels with different pore sizes between 70 to 1000Å with particle size distribution ranging from 20-45 µm to 75-200 µm for chromatography purposes as a replacement of irregular silicas.
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