SiliaChrom® - dt Reversed Phase HPLC Columns
SiliaChrom dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series. This phase has no metal content, in order to avoid secondary interaction with basic or ion species. SiliaChrom dt C18 is made with a true universal reversed-phase sorbent that can be used for separation of acidic, basic and neutral molecules. It is suitable for analysis of stringent QA/QC testing because it has a superior performance than any C18 phases in almost every aspects. SiliaChrom dt C18 is an interesting environmental and pharmaceutical phases.
Main Characteristics
- Enhances retention of hydrophilic molecules
- Low bleed and high sensitivity for LC-MS
- Extremely low metal content (< 10 ppm)
- Good tolerance for directly injected biological matrix (dirty samples)
- Higher surface area
- Partially endcapped
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GENERAL SiliaChrom dt CHARACTERISTICS
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| Columns | Pore Size | Particle Size | Surface Area | Carbon Loading |
| C18 | 100 Å | 2.5, 3, 5 µm | 410-441 m2/g | 18% |
| Columns | pH Range | Temperature Limit* | Pressure Limit | USP Code |
| C18 | 1,5-9,0 | 65° C | 5000 psi | L01 |
* At pH range 5 to 7,5
Available column types: Fast analysis, Analytical, Semi-Preparative, Preparative
Application Note
SiliaChrom dt C18 - Assay for QC Testing of Blood Pressure and Cholesterol Medication
Evaluation of Resolution and Peak Shape of Chlorinated Herbicides
SiliaChrom dt C18, 5 µm, 4,6x150 mm (SiliCycle P/N: HPC-14303-15N)
5 mM potassium phosphate monobasic (adjust to pH 2,5 with H3PO4)/ACN
Temperature: 23°C
Flow rate: 1,0 mL/min
Detector: UV at 210 nm
Injection volume: 10 µL
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COLUMN PERFORMANCE RESULTS
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| Chlorinated Herbicides | Retention Time | Peak Asymmetry Factor | Theoretical Plates |
| (min) | (USP) | (USP) | |
| Dicamba | 7,05 | 1,01 | 12746 |
| Atrazine | 9,77 | 1,00 | 12546 |
| 2,4-Dichlorophenoxyacetic acid | 10,63 | 1,02 | 12736 |
3 Item(s)
List Grid
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SiliaChrom® Preparative HPLC Columns dt C18
SiliaChrom® dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series.
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SiliaChrom® Narrow Bore HPLC Columns dt C18
SiliaChrom® dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series.
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SiliaChrom® Analytical HPLC Columns dt C18
SiliaChrom® dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series.
3 Item(s)
List Grid
- Application Fields
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- Bio-Pharma (3)
- Forensic (3)
- Pharmaceutical (3)
- Type of Column
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- Narrow Bore Columns (1)
- Analytical Columns (1)
- Preparative Columns (1)
- Chromatographic Mode
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- Reversed Phases (3)
- USP Code
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- L1 (3)
- Family / Formats
- Phases
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- dt C18 (3)
- Pore Size
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- 100 Å (3)
- Endcapping
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- Endcapped (3)
- Internal Diameter
- Particle Size
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