SiliaChrom®- XDB HPLC Columns
SiliaChrom - XDB C18 and C8 are specially designed to present high pore size and low surface area for the separation of large hydrophobic molecules. The relatively low surface area allows a shorter retention time for that kind of compounds. SiliaChrom XDB C18 and C8 present the lowest carbon loading of all SiliaChrom HPLC series. SiliaChrom XDB phases are ideal for separation of barbiturates, fat-soluble vitamins, fatty acids and steroïds.
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Main Characteristics
- Better choice for molecules > 500 Dalton
- High loading capacity
- Wide pH range: 1,5 to 10,0
- Double endcapped
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GENERAL SiliaChrom XDB CHARACTERISTICS
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| Columns | Pore Size | Particle Size | Surface Area | Carbon Loading |
| C18 | 150Å | 3, 5 µm | 200 m2/g | 15% |
| C8 | 150Å | 3, 5 µm | 200 m2/g | 8% |
| Columns | pH Range | Temperature Limit* | Pressure Limit | USP Code |
| C18 | 1,5-10,0 | 60°C | 5500 psi | L01 |
| C8 | 1,5-10,0 | 60°C | 5500 psi | L07 |
* At pH range 5 to 7,5
Available column types: Analytical, Semi-Preparative, Preparative
Application Note
Evaluation of Resolution and Peak Shape of an Highly Hydrophobic Domestic Insecticid
SiliaChrom XDB C18, 5 µm, 4,6x150 mm
ACN/H2O (90/10)
Temperature: 23,0°C
Flow Rate: 1,00 mL/min
Detector: UV 235 nm
Injection Volume: 1 µL
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COLUMN PERFORMANCE RESULTS
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| Compounds | Retention Time | Peak Asymmetry Factor | Theoretical Plates |
| (min) | (USP) | (USP) | |
| Uracil | 1,72 | 1,26 | 5936 |
| Bioresmethrin | 4,34 | 1,03 | 14090 |
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