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Frequently Asked Questions about Functionalized Silica

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Find here all frequently asked questions about our SiliaBond functionalized chromatographic phases.

functionalized silica

 

General Information

What is endcapping?

Are all functionalized products endcapped?

What is the difference between a monomeric and a polymeric phase?

How should functionalized silica be handled?

Can functionalized silica be packed?

 

Stability

What are the storage conditions for the functionalized silica?

Does functionalized silica dissolve in methanol or any other standard organic solvents?

What will happen to my functionalized silica gel if I do not work in the recommended pH scale (between 2 and 10)?

What is the thermal stability of your chromatographic phases?

Do I have to work in the dark using silver nitrate (AgNO3) silica-based products?

 

Applications

I would like to try using a fluorinated phase, which one should I select?

Which functionalized silica gel is the most appropriate to purify/analyze sugars?

Silver nitrate (AgNO3) silica-based products can be used for what type of separations?

What is the Catch and Release technique?

 

Reversed-Phases

What is the proper way to condition a reversed-phase glass column?

My compound binds too strongly on my C18 silica gel, what can I do?

Does a higher %C for a C18 phase necessarily imply a better retention of hydrophobic compounds?

 

Ion Exchange Mode (IEC)

What do the abbreviations WAX, SAX, WCX, and SCX mean in IEC?

How do we select the eluent in IEC?

What is the concept behind the separation of the products in IEC?

Why is the counter-ion on a SAX phase important?

 

HILIC Mode

What is the principle of the HILIC mode in chromatography?

When should we choose the HILIC mode over either the normal or the reversed-phase ones?

Why and when should I add a buffer in my eluent in HILIC mode?

 

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  1. Poster - SiliCycle's Most Popular Functionalized Silicas

    SiliCycle's Most Popular Functionalized Silicas

    Poster - Download our free poster of the most popular functionalized silicas and their main purification applications whether for chromatography, metal & organic scavenging or as reagents & oxidants.
  2. Solutions For Purification & Chromatography (BROPUR)

    Solutions For Purification and Chromatography (BROPUR)

    Brochure, 48 pages. Product lines: SiliaFlash Bulk Irregular Silica for Chromatography, SiliaSphere Bulk Spherical Silica for Chromatography, SiliaBond Chromatographic Phases, SiliaSep Flash Cartridges and SiliaPlate TLC Plates

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