Frequently Asked Questions about Functionalized Silica
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Find here all frequently asked questions about our SiliaBond functionalized chromatographic phases.
General Information
◼ What is endcapping?
◼ Are all functionalized products endcapped?
◼ What is the difference between a monomeric and a polymeric phase?
◼ How should functionalized silica be handled?
◼ Can functionalized silica be packed?
Stability
◼ What are the storage conditions for the functionalized silica?
◼ Does functionalized silica dissolve in methanol or any other standard organic solvents?
◼ What will happen to my functionalized silica gel if I do not work in the recommended pH scale (between 2 and 10)?
◼ What is the thermal stability of your chromatographic phases?
◼ Do I have to work in the dark using silver nitrate (AgNO3) silica-based products?
Applications
◼ I would like to try using a fluorinated phase, which one should I select?
◼ Which functionalized silica gel is the most appropriate to purify/analyze sugars?
◼ Silver nitrate (AgNO3) silica-based products can be used for what type of separations?
◼ What is the Catch and Release technique?
Reversed-Phases
◼ What is the proper way to condition a reversed-phase glass column?
◼ My compound binds too strongly on my C18 silica gel, what can I do?
◼ Does a higher %C for a C18 phase necessarily imply a better retention of hydrophobic compounds?
Ion Exchange Mode (IEC)
◼ What do the abbreviations WAX, SAX, WCX, and SCX mean in IEC?
◼ How do we select the eluent in IEC?
◼ What is the concept behind the separation of the products in IEC?
◼ Why is the counter-ion on a SAX phase important?
HILIC Mode
◼ What is the principle of the HILIC mode in chromatography?
◼ When should we choose the HILIC mode over either the normal or the reversed-phase ones?
◼ Why and when should I add a buffer in my eluent in HILIC mode?
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SiliCycle's Most Popular Functionalized Silicas
Poster - Download our free poster of the most popular functionalized silicas and their main purification applications whether for chromatography, metal & organic scavenging or as reagents & oxidants. -
Solutions For Purification and Chromatography (BROPUR)
Brochure, 48 pages. Product lines: SiliaFlash Bulk Irregular Silica for Chromatography, SiliaSphere Bulk Spherical Silica for Chromatography, SiliaBond Chromatographic Phases, SiliaSep Flash Cartridges and SiliaPlate TLC Plates