What is HPLC?
HPLC stands for High-Performance Liquid Chromatography (formerly referred to as High-Pressure Liquid Chromatography).
It is a chromatographic technique used to separate the components in a mixture, to identify each component, and to quantify each component.
In general, the method involves a liquid sample being passed over a solid adsorbent material packed into a column using a flow of liquid solvent. Each analyte in the sample interacts slightly differently with the adsorbent material, thus retarding the flow of the analytes. If the interaction is weak, the analytes flow off the column in a short amount of time, and if the interaction is strong, then the elution time is long.