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Unable to see any spots on the TLC?

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Possible Solutions:

  • If you have not been able to visualize any spots on your TLC using UV light, try another method; maybe your compound is not UV-active.
  • Maybe your sample is too diluted. Try to apply several times your sample on the same spot (do not forget to dry solvent between each application for optimal results) or to concentrate your solution.
  • Make sure the solvent level inside the tank is lower than the spotting line to avoid sample dissolution by the eluent.