SiliaChrom^®- CHIRAL HPLC Columns

Operating procedure of SiliaChrom Chiral Cellulose T-DPC and SiliaChrom Chiral Cellulose T-MB

Mobile phases:

¹Alkane: n-hexane, iso-hexane or n-heptane. Some small selectivity differences may sometimes be found.

²The retention is generally shorter with Ethanol than with 2-propanol.
  The retention is generally shorter with a higher alcohol content.
  The use of other alcohols such as 1-propanol, 1-BuOH, 2-BuOH ... is possible, buth effectiveness cannot be guaranteed.

³ Due to limited miscibility of MeOH in Alkane, it is necessary to add an appropriate volume of EtOH together with MeOH in order to obtain an homogeneous solvent mixture. A maximum of 5% MeOH in n-hexane may be used without adding EtOH.

⁴ Ideal starting conditions: MeOH/EtOH 50:50 (v/v) when alcohol mixtures are required.

⁵ The use of polar solvents as 100% methanol is possible with SiliaChrom Chiral Cellulose T-DPC column. Nevertheless, once the column is transfered to a polar mode it should be dedicated to this specific application.
To safely transfer the column from hexane to methanol it is strongly recommended to use 100% 2-propanol as a transition mobile phase at a low flow rate (high viscosity of 2-propanol).

Modifiers:
For basic or acidic samples, it is necessary to add a modifier into the mobile phase in order to achieve the chiral separation: ⁶ for primary amines mainly and ⁷ for primary amino alcohols mainly.

Operating procedure of SiliaChrom Chiral Amylose T-DPC

Mobile phases:

¹ Alkane: n-hexane, iso-hexane or n-heptane. Some small selectivity differences may sometimes be found.

² The range Hexane/EtOH 85/15 ~ 40/60 may adversely affect baseline stability dur to polymer leakage.
To safely transfer the column from 15% EtOH to 60% EtOH, it is strongly recommended to use 100% 2-propanol as a transition mobile phase at a low flow rate (high viscosity of 2-propanol).
The retention is generally shorter with Ethanol than with 2-propanol.
The retention is generally shorter with a higher alcohol content.
The use of other alcohols such as 1-propanol, 1-BuOH, 2-BuOH ... is possible, buth effectiveness cannot be guaranteed. Do not use mobile phases containing more than 15% of these alcohols.

³ Due to the limited miscibility of MeOH in Alkane, it is necessary to add an appropriate volume of EtOH together with MeOH in order to obtain an homogeneous solvent mixture. A maximum of 5% MeOH in n-hexane only may used without adding EtOH.

⁴ Ideal starting conditions: MeOH/EtOH 50:50 (v/v) when alcohol mixtures are required.

⁵ The use of polar solvents as 100% methanol or 100% acetonitrile is possible with SiliaChrom Chiral Amylose T-DPC column. Nevertheless, once the column is transfered to a polar mode it should be dedicated to this specific application.
To safely transfer the column from n-hexane to methanol or acetonitrile or between different polar solvents, it is strongly recommended to use 100% 2-propanol as a transition mobile phase at a low flow rate (high viscosity of 2-propanol).

⁶ More than 15% of alcohol other than 2-propanol, in acetonitrile may destroy the column. Compatibility of such mixtures with the chiral stationary phase cannot be guaranteed.

⁷ The use of other alcohols such as 1-propanol, 1-BuOH, 2-BuOH ... is possible, buth effectiveness  cannot be guaranteed. Do not use mobile phases containing more than 15% of these alcohols.

Modifiers:

For basic or acidic samples, it is necessary to add a modifier into the mobile phase in order to achieve the chiral separation: ⁸ for primary amines mainly and ⁹ for primary amino alcohols mainly.

Application Note

SiliaChrom Chiral Amylose T-DPC - Enantiomeric Separation of L & D-val-PMB