SiliaChrom® - dt Reversed Phase HPLC Columns
SiliaChrom dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series. This phase has no metal content, in order to avoid secondary interaction with basic or ion species. SiliaChrom dt C18 is made with a true universal reversed-phase sorbent that can be used for separation of acidic, basic and neutral molecules. It is suitable for analysis of stringent QA/QC testing because it has a superior performance than any C18 phases in almost every aspects. SiliaChrom dt C18 is an interesting environmental and pharmaceutical phases.

Main Characteristics
- Enhances retention of hydrophilic molecules
- Low bleed and high sensitivity for LC-MS
- Extremely low metal content (< 10 ppm)
- Good tolerance for directly injected biological matrix (dirty samples)
- Higher surface area
- Partially endcapped
More Information
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GENERAL SiliaChrom dt CHARACTERISTICS
|
| Columns |
Pore Size |
Particle Size |
Surface Area |
Carbon Loading |
| C18 |
100 Å |
2.5, 3, 5 µm |
410-441 m2/g |
18% |
| Columns |
pH Range |
Temperature Limit* |
Pressure Limit |
USP Code |
| C18 |
1,5-9,0 |
65° C |
5000 psi |
L01 |
* At pH range 5 to 7,5
Available column types: Fast analysis, Analytical, Semi-Preparative, Preparative
Application Note
SiliaChrom dt C18 - Assay for QC Testing of Blood Pressure and Cholesterol Medication
Evaluation of Resolution and Peak Shape of Chlorinated Herbicides
SiliaChrom dt C18, 5 µm, 4,6x150 mm (SiliCycle P/N: HPC-14303-15N)
5 mM potassium phosphate monobasic (adjust to pH 2,5 with H3PO4)/ACN
Temperature: 23°C
Flow rate: 1,0 mL/min
Detector: UV at 210 nm
Injection volume: 10 µL

|
COLUMN PERFORMANCE RESULTS
|
| Chlorinated Herbicides |
Retention Time |
Peak Asymmetry Factor |
Theoretical Plates |
|
(min) |
(USP) |
(USP) |
| Dicamba |
7,05 |
1,01 |
12746 |
| Atrazine |
9,77 |
1,00 |
12546 |
| 2,4-Dichlorophenoxyacetic acid |
10,63 |
1,02 |
12736 |
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SiliaChrom® dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series.
-
SiliaChrom® dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series.
-
SiliaChrom® dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series.
-
SiliaChrom® dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series.