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SiliaChrom®- SB HPLC Columns

The SiliaChrom SB C8 and C18 are specially designed for the separation of polar and mid-polar compounds from low to medium pH. A class of columns offering excellent performance for LC-MS applications. More Information

The surface of SiliaChrom SB C8 and C18 are treated with an organic form of silicon to increase the number of silanol groups on the surface. After this, the surface is bonded with an octadecylsilane with two different fonctions. One of them is a protecting group that shields the area under it and protects the surface from an acid attack from the mobile phase.

sb-c8-c18.jpg

Main Characteristics

  • Extremely low pH Limits (0,5-7,5)
  • Extremely low bleed for LC-MS applications under acidic conditions
  • Compatible with mobile phases ranging 100% aqueous to 100% organic
  • Non-endcapped

More Information

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GENERAL SiliaChrom SB CHARACTERISTICS
Columns Pore Size Particle Size Surface Area Carbon Loading
C18 150Å 3, 5 µm 200-220 m2/g 12%
C8 150Å 3, 5 µm 200-220 m2/g 7%
Columns pH Range Temperature Limit* Pressure Limit USP Code
C18 0,5-7,5 50°C 4500 psi L01
C8 0,5-7,5 50°C 4500 psi L07

* At pH range 5 to 7,5
Available column types:
Fast analysis, Analytical, Semi-Preparative, Preparative

 

Application Notes

Melamine in Food

LC-MS Pseudoephedrine in Plasma

LC-MS Hydrochlorothiazide in Human Plasma

LC-MS Glipizide in Human Plasma

LC-MS Amlodipine in Bovine Serum

LC-MS Propranolol in Bovine Serum

Evaluation Testing for Low pH

Column Performance Evaluation

Siliachrom sbc18 ethylbenzene

SiliaChrom SB C18, 5 µm, 4,6x150 mm (SiliCycle P/N: H101805H-N150)
ACN:Water:TFA (59:39:2) pH = 1,0
Detector: UV 270 nm
Temperature: 23,0°C
Flow rate: 1,00 mL/min

COLUMN PERFORMANCE RESULTS
Time Retention Time Tailling Factor Theoretical Plates
(h) Ethylbenzene (min) Ethylbenzene (USP) Ethylbenzene (USP)
0 5,91 1,01 14014
24 5,89 1,02 14085
48 5,77 1,02 14023
72 5,83 1,02 14076
96 5,85 1,01 14087
120 5,84 1,02 14050
SD 0,05 0,01 32
Mean 5,85 1,02 14056
RSD (%) 0,84 0,51 0,23

 

Poster

New Generation of HPLC Silica-Based C18 Column for Both Highly Acidic and Basic pH: The SiliaChrom SB C18 and SiliaChrom XT C18 Fidelity

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  1. SiliaChrom® Preparative HPLC Columns SB C18

    SiliaChrom® Preparative HPLC Columns SB C18

    SiliaChrom® SB C18 has the particularity that the silane carries two different functions:

    • The C18 Hydrophobic function

    • Transversal hindered isopropyl groups which protect the surface from an acidic attack.

    This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)

  2. SiliaChrom® Semi-Preparative HPLC Columns SB C8-300

    SiliaChrom® Semi-Preparative HPLC Columns SB C8-300

    SiliaChrom® C8 reverse phase functionalization is completely identical to the SB C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  3. SiliaChrom® Semi-Preparative HPLC Columns SB C18-300

    SiliaChrom® Semi-Preparative HPLC Columns SB C18-300

    SiliaChrom® C18 reverse phase functionalization is completely identical to the SB C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  4. SiliaChrom® Semi-Preparative HPLC Columns SB C8

    SiliaChrom® Semi-Preparative HPLC Columns SB C8

    SiliaChrom® SB C18 has the particularity that the silane carries two different functions:

    • The C18 Hydrophobic function

    • Transversal hindered isopropyl groups which protect the surface from an acidic attack.

    This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)

  5. SiliaChrom® Semi-Preparative HPLC Columns SB C18

    SiliaChrom® Semi-Preparative HPLC Columns SB C18

    SiliaChrom® SB C18 has the particularity that the silane carries two different functions:

    • The C18 Hydrophobic function

    • Transversal hindered isopropyl groups which protect the surface from an acidic attack.

    This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)

  6. SiliaChrom® Preparative HPLC Columns SB C18-300

    SiliaChrom® Preparative HPLC Columns SB C18-300

    SiliaChrom® C18 reverse phase functionalization is completely identical to the SB C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  7. SiliaChrom® Preparative HPLC Columns SB C8-300

    SiliaChrom® Preparative HPLC Columns SB C8-300

    SiliaChrom® C8 reverse phase functionalization is completely identical to the SB C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  8. SiliaChrom® Narrow Bore HPLC Columns SB C18

    SiliaChrom® Narrow Bore HPLC Columns SB C18

    SiliaChrom® SB C18 has the particularity that the silane carries two different functions:

    • The C18 Hydrophobic function

    • Transversal hindered isopropyl groups which protect the surface from an acidic attack.

    This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)

  9. SiliaChrom® Narrow Bore HPLC Columns SB C8

    SiliaChrom® Narrow Bore HPLC Columns SB C8

    SiliaChrom® SB C18 has the particularity that the silane carries two different functions:

    • The C18 Hydrophobic function

    • Transversal hindered isopropyl groups which protect the surface from an acidic attack.

    This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)

  10. SiliaChrom® Narrow Bore HPLC Columns SB C18-300

    SiliaChrom® Narrow Bore HPLC Columns SB C18-300

    SiliaChrom® C18 reverse phase functionalization is completely identical to the SB C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  11. SiliaChrom® Narrow Bore HPLC Columns SB C8-300

    SiliaChrom® Narrow Bore HPLC Columns SB C8-300

    SiliaChrom® C8 reverse phase functionalization is completely identical to the SB C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  12. SiliaChrom® Analytical HPLC Columns SB C18

    SiliaChrom® Analytical HPLC Columns SB C18

    SiliaChrom® SB C18 has the particularity that the silane carries two different functions:

    • The C18 Hydrophobic function

    • Transversal hindered isopropyl groups which protect the surface from an acidic attack.

    This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)

  13. SiliaChrom® Analytical HPLC Columns SB C8

    SiliaChrom® Analytical HPLC Columns SB C8

    SiliaChrom® SB C18 has the particularity that the silane carries two different functions:

    • The C18 Hydrophobic function

    • Transversal hindered isopropyl groups which protect the surface from an acidic attack.

    This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)

  14. SiliaChrom® Analytical HPLC Columns SB C8-300

    SiliaChrom® Analytical HPLC Columns SB C8-300

    SiliaChrom® C8 reverse phase functionalization is completely identical to the SB C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

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