The SiliaChrom SB C8 and C18 are specially designed for the separation of polar and mid-polar compounds from low to medium pH. A class of columns offering excellent performance for LC-MS applications. More Information
The surface of SiliaChrom SB C8 and C18 are treated with an organic form of silicon to increase the number of silanol groups on the surface. After this, the surface is bonded with an octadecylsilane with two different fonctions. One of them is a protecting group that shields the area under it and protects the surface from an acid attack from the mobile phase.
Main Characteristics
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GENERAL SiliaChrom SB CHARACTERISTICS
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|---|---|---|---|---|
| Columns | Pore Size | Particle Size | Surface Area | Carbon Loading |
| C18 | 150Å | 3, 5 µm | 200-220 m2/g | 12% |
| C8 | 150Å | 3, 5 µm | 200-220 m2/g | 7% |
| Columns | pH Range | Temperature Limit* | Pressure Limit | USP Code |
| C18 | 0,5-7,5 | 50°C | 4500 psi | L01 |
| C8 | 0,5-7,5 | 50°C | 4500 psi | L07 |
* At pH range 5 to 7,5
Available column types: Fast analysis, Analytical, Semi-Preparative, Preparative
Application Notes
LC-MS Pseudoephedrine in Plasma
LC-MS Hydrochlorothiazide in Human Plasma
LC-MS Glipizide in Human Plasma
LC-MS Amlodipine in Bovine Serum
LC-MS Propranolol in Bovine Serum
Column Performance Evaluation
SiliaChrom SB C18, 5 µm, 4,6x150 mm (SiliCycle P/N: H101805H-N150)
ACN:Water:TFA (59:39:2) pH = 1,0
Detector: UV 270 nm
Temperature: 23,0°C
Flow rate: 1,00 mL/min
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COLUMN PERFORMANCE RESULTS
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|---|---|---|---|
| Time | Retention Time | Tailling Factor | Theoretical Plates |
| (h) | Ethylbenzene (min) | Ethylbenzene (USP) | Ethylbenzene (USP) |
| 0 | 5,91 | 1,01 | 14014 |
| 24 | 5,89 | 1,02 | 14085 |
| 48 | 5,77 | 1,02 | 14023 |
| 72 | 5,83 | 1,02 | 14076 |
| 96 | 5,85 | 1,01 | 14087 |
| 120 | 5,84 | 1,02 | 14050 |
| SD | 0,05 | 0,01 | 32 |
| Mean | 5,85 | 1,02 | 14056 |
| RSD (%) | 0,84 | 0,51 | 0,23 |
Poster
SiliaChrom® SB C18 has the particularity that the silane carries two different functions:
• The C18 Hydrophobic function
• Transversal hindered isopropyl groups which protect the surface from an acidic attack.
This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)
SiliaChrom® C8 reverse phase functionalization is completely identical to the SB C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
SiliaChrom® C18 reverse phase functionalization is completely identical to the SB C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
SiliaChrom® SB C18 has the particularity that the silane carries two different functions:
• The C18 Hydrophobic function
• Transversal hindered isopropyl groups which protect the surface from an acidic attack.
This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)
SiliaChrom® SB C18 has the particularity that the silane carries two different functions:
• The C18 Hydrophobic function
• Transversal hindered isopropyl groups which protect the surface from an acidic attack.
This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)
SiliaChrom® C18 reverse phase functionalization is completely identical to the SB C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
SiliaChrom® C8 reverse phase functionalization is completely identical to the SB C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
SiliaChrom® SB C18 has the particularity that the silane carries two different functions:
• The C18 Hydrophobic function
• Transversal hindered isopropyl groups which protect the surface from an acidic attack.
This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)
SiliaChrom® SB C18 has the particularity that the silane carries two different functions:
• The C18 Hydrophobic function
• Transversal hindered isopropyl groups which protect the surface from an acidic attack.
This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)
SiliaChrom® C18 reverse phase functionalization is completely identical to the SB C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
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