SiliaChrom®- XDB2 HPLC Columns
SiliaChrom XDB2 C18 is designed to present a mid-hydrophobic C18 phase with 18% of carbon loading like most of the popular reversed-phase HPLC columns on the market. This phase presents a balanced hydrophobic adsorption in order to avoid excessive retention of hydrophobic compounds.
Main Characteristics
- Great column-to-column and batch-to-batch reproducibility (popular for QC/QA laboratory)
- Typical average value for carbon loading (18%)
- Good peak shape for basic, acidic and neutral analytes
- Stronger separation power for isomers
- Double endcapped
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GENERAL SiliaChrom XDB2 CHARACTERISTICS
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| Columns | Pore Size | Particle Size | Surface Area | Carbon Loading |
| C18 | 100Å | 3, 5 µm | 380 m2/g | 18% |
| Columns | pH Range | Temperature Limit* | Pressure Limit | USP Code |
| C18 | 1,5-9,0 | 65°C | 5000 psi | L01 |
* At pH range 5 to 7,5
Available column types: Fast analysis, Analytical, Semi-Preparative, Preparative
Application Note
Evaluation of SiliaChrom XDB2 C18 by USP and NIST Approches
(Pharmacopeia Forum 31 (2005) 637, NIST SRM 870 certificate issue: 19th december 2002 valid until: 30th september 2010)
SiliaChrom XDB2 C18, 5 µm, 4,6x150 mm
Methanol/10 mM potassium phosphate monobasic + 10 mM potassium phosphate dibasic (pH: 7,00) (80/20)
Temperature: 23,0°C
Flow Rate: 1,00 mL/min
Detector: UV 254 nm
Injection Volume: 1 µL
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COLUMN PERFORMANCE RESULTS
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| Compounds | Retention Time | Peak Asymmetry Factor | Theoretical Plates |
| (min) | (USP) | (USP) | |
| Uracil | 1,61 | 1,24 | 4618 |
| Toluene | 4,73 | 1,04 | 12858 |
| Ethylbenzene | 6,19 | 1,00 | 13633 |
| Quinirazine | 11,18 | 1,03 | 12277 |
| Amitriptyline | 13,53 | 1,29 | 9451 |
1 Item(s)
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SiliaChrom® Narrow Bore HPLC Columns XDB2 C18
SiliaChrom® XDB2 C18 is grafted on ultrapure spherical silica whose surface was activated to create a high concentration of OH before grafting the C18 function. The residual carbon load is lower than the SiliaChrom XDB1 equivalent.
1 Item(s)
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- Type of Column
- Chromatographic Mode
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- Reversed Phases (1)
- USP Code
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- L1 (1)
- Family / Formats
- Phases
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- XDB2 C18 (1)
- Pore Size
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- 100 Å (1)
- Endcapping
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- Endcapped (1)
- Internal Diameter
- Particle Size
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