SiliaChrom®- XT & XT Fidelity HPLC Columns
The SiliaChrom XT C18 column is designed for application to be run at very high pH (up to 12,0) at room temperature but also suitable for low pH (down to 1,5). The other one, SiliaChrom XT C18 Fidelity, is used at high pH conditions with a higher thermal capacity. The only difference between SiliaChrom XT and SiliaChrom XT Fidelity is the way how the HPLC column is packed (proprietary) given more robustness at high pH and temperature.
The key is to have a hybrid particle or hybrid surface to reduce de solubility of the silica at high pH. In fact, the SiliaChrom XT is silica coated with a prepolymer of methyltriethoxysilane/tetraethoxysilane, followed by special thermal treatment to get a rigid surface that is less soluble than silica itself.
Main Characteristics
- Excellent durability at high pH
- Ideal for basic compounds
- High thermal stability
- Ideal for auto-purification (Prep. LC-MS)
- Double endcapped
- Best HPLC column for Metabolite Study
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GENERAL SiliaChrom XT CHARACTERISTICS
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| Columns | Pore Size | Particle Size | Surface Area | Carbon Loading |
| C18 | 150Å | 3, 5 µm | 200 m2/g | 15% |
| C18 Fidelity | 150Å | 3, 5 µm | 200 m2/g | 21% |
| Columns | pH Range | Temperature Limit* | Pressure Limit | USP Code |
| C18 | 1,5-12,0 | 60°C | 5000 psi | L01 |
| C18 Fidelity | 1,5-12,0 | 60°C | 5000 psi | L01 |
* At pH range 5 to 7,5
Available column types: Fast analysis, Analytical, Semi-Preparative, Preparative
Application Note
Evaluation Testing for High pH:
SiliaChrom XT C18 Fidelity, 5 µm, 4,6x150 mm (SiliCycle P/N: HPC-11005-5N)
ACN:Water:TEA (54,9:44,9:0,2) pH = 11,50
UV 270 nm
Column temperature: 23,0°C
Flow: 1,00 mL/min
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COLUMN PERFORMANCE RESULTS
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|---|---|---|---|
| Time | Retention Time | Tailling Factor | Theoretical Plates |
| (h) | Ethylbenzene (min) | Ethylbenzene (USP) | Ethylbenzene (USP) |
| 0 | 13,35 | 1,01 | 13623 |
| 24 | 13,29 | 1,01 | 13648 |
| 48 | 13,27 | 1,01 | 13689 |
| 72 | 13,25 | 1,00 | 13604 |
| 96 | 13,24 | 1,00 | 13649 |
| 120 | 13,28 | 1,00 | 13582 |
| SD | 0,04 | 0,01 | 38 |
| Mean | 13,28 | 1,01 | 13633 |
| RSD (%) | 0,29 | 0,54 | 0,28 |
Poster
2 Item(s)
List Grid
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SiliaChrom® Semi-Preparative HPLC Columns XT C18 Fidelity
SiliaChrom® XT Fidelity C18 has a hybrid surface composed from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, has an incomparable resistance to extreme high pH conditions (up to 12). The only difference between SiliaChrom XT C18 and SiliaChrom XT Fidelity C18 is the way they are packed, giving Fidelity columns more robustness at high pH and temperature.
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SiliaChrom® Semi-Preparative HPLC Columns XT C18
SiliaChrom® XT C18 has a hybrid surface made from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, presents a very high resistance to extreme high pH conditions (up to 12).
2 Item(s)
List Grid
- Application Fields
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- Bio-Pharma (2)
- Type of Column
- Chromatographic Mode
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- Reversed Phases (2)
- USP Code
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- L1 (2)
- Family / Formats
- Phases
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- XT C18 (1)
- XT C18 Fidelity (1)
- Pore Size
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- 150 Å (2)
- Endcapping
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- Endcapped (2)
- Length
- Particle Size
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