
SiliaChrom®- HPLC Columns
SiliCycle manufactures a variety of HPLC columns for reversed and normal phases applications. The SiliaChrom series contain more than 20 different phases produced in our own laboratories, and we continue to develop additional unique and powerful HPLC columns. In fact, you can be completely assured of the quality, from raw material synthesis through packing, testing and customer applications.
SiliaChrom HPLC columns are made of highly pure spherical particles. One of the most remarkable properties of every SiliaChrom column is that all types of organic compounds (acidic, basic and neutral) will have optimal peak symmetry with excellent efficiency across the whole applicable pH range. Lot-to-lot reproducibility is ensured by a stable bonding and endcapping process. All columns are packed using a consistent slurry packing process to achieve uniform and stable bed for maximum column efficiency, long lifetime and column-to-column reproducibility.
SiliaChrom HPLC Columns
| Main characteristics > | Benefits for the column users |
|---|---|
| Broad pH range (0,8-12,0) | Flexibility for method development |
| Compatible with 100% aqueous mobile phase | Maximum versatility |
| High surface coverage | Tolerance to large volume and mass injected |
| Minimum buffer concentration needed | Better sensitivity for LC-MS and column lifetime |
| Low bleed | High sensitivity for LC-MS |
| Wide variety of chemistries available | Broad selection of polarity |
| Excellent column effiency | Better and faster separation |
| Long column lifetime | Lower cost |
| Thightest specification | Better confidence in HPLC results |
Standard HPLC Columns
SiliaChrom HPLC columns are available in Narrow Bore, Analytical, Semi-Preparative, and Preparative formats.
| SiliaChrom HPLC Standard Column Dimensions | ||||||||
|---|---|---|---|---|---|---|---|---|
| SiliaChrom HPLC Column Length (mm) | ||||||||
20 |
30 |
50 |
100 |
150 |
200 |
250 |
||
| Particle Size (µm) | 2.5, 3 & 5 |
2.5, 3 & 5 |
2.5, 3, 5, 7 & 10 |
2.5, 3, 5, 7, 10 & 20 |
2.5, 3, 5, 7, 10 & 20 |
5 & 10 |
2.5, 3, 5, 7, 10 & 20 |
|
| SiliaChrom HPLC Column Internal Diameter (mm) |
2.0 | S |
S |
S |
S |
S |
C |
C |
| 2.1 | S |
S |
S |
S |
S |
C |
C |
|
| 3.0 | C |
S |
S |
S |
S |
C |
C |
|
| 4.6 | C |
S |
S |
S |
S |
S |
S |
|
| 10 | C |
C |
C |
S |
S |
S |
S |
|
| 20 | C |
C |
C |
C |
S |
S |
S |
|
| 30 | C |
C |
C |
S |
S |
S |
S |
|
| 50 | C |
C |
C |
S |
S |
S |
S |
|
| 100 | C |
C |
C |
C |
C |
C |
C |
|
| S = Standard C = Custom | ||||||||
Distribution
SiliCycle is also an authorized distributor of HPLC columns from other suppliers:
![]() nacalai tesque inc. - Specialized HPLC Columns Sugar-D, Cholester, HILIC, π-NAP |
![]() SIELC Technologies - Mixed Mode Stationary Phases HPLC Columns |
-
SiliaChrom® Preparative Guard Cartridge SB C8
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.
-
SiliaChrom® Semi-Preparative HPLC Columns SB C8
SiliaChrom® SB C18 has the particularity that the silane carries two different functions:
• The C18 Hydrophobic function
• Transversal hindered isopropyl groups which protect the surface from an acidic attack.
This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)
-
SiliaChrom® Semi-Preparative Guard Cartridge SB C8
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.







