
SiliaChrom®- HPLC Columns
SiliCycle manufactures a variety of HPLC columns for reversed and normal phases applications. The SiliaChrom series contain more than 20 different phases produced in our own laboratories, and we continue to develop additional unique and powerful HPLC columns. In fact, you can be completely assured of the quality, from raw material synthesis through packing, testing and customer applications.
SiliaChrom HPLC columns are made of highly pure spherical particles. One of the most remarkable properties of every SiliaChrom column is that all types of organic compounds (acidic, basic and neutral) will have optimal peak symmetry with excellent efficiency across the whole applicable pH range. Lot-to-lot reproducibility is ensured by a stable bonding and endcapping process. All columns are packed using a consistent slurry packing process to achieve uniform and stable bed for maximum column efficiency, long lifetime and column-to-column reproducibility.
SiliaChrom HPLC Columns
| Main characteristics > | Benefits for the column users |
|---|---|
| Broad pH range (0,8-12,0) | Flexibility for method development |
| Compatible with 100% aqueous mobile phase | Maximum versatility |
| High surface coverage | Tolerance to large volume and mass injected |
| Minimum buffer concentration needed | Better sensitivity for LC-MS and column lifetime |
| Low bleed | High sensitivity for LC-MS |
| Wide variety of chemistries available | Broad selection of polarity |
| Excellent column effiency | Better and faster separation |
| Long column lifetime | Lower cost |
| Thightest specification | Better confidence in HPLC results |
Standard HPLC Columns
SiliaChrom HPLC columns are available in Narrow Bore, Analytical, Semi-Preparative, and Preparative formats.
| SiliaChrom HPLC Standard Column Dimensions | ||||||||
|---|---|---|---|---|---|---|---|---|
| SiliaChrom HPLC Column Length (mm) | ||||||||
20 |
30 |
50 |
100 |
150 |
200 |
250 |
||
| Particle Size (µm) | 2.5, 3 & 5 |
2.5, 3 & 5 |
2.5, 3, 5, 7 & 10 |
2.5, 3, 5, 7, 10 & 20 |
2.5, 3, 5, 7, 10 & 20 |
5 & 10 |
2.5, 3, 5, 7, 10 & 20 |
|
| SiliaChrom HPLC Column Internal Diameter (mm) |
2.0 | S |
S |
S |
S |
S |
C |
C |
| 2.1 | S |
S |
S |
S |
S |
C |
C |
|
| 3.0 | C |
S |
S |
S |
S |
C |
C |
|
| 4.6 | C |
S |
S |
S |
S |
S |
S |
|
| 10 | C |
C |
C |
S |
S |
S |
S |
|
| 20 | C |
C |
C |
C |
S |
S |
S |
|
| 30 | C |
C |
C |
S |
S |
S |
S |
|
| 50 | C |
C |
C |
S |
S |
S |
S |
|
| 100 | C |
C |
C |
C |
C |
C |
C |
|
| S = Standard C = Custom | ||||||||
Distribution
SiliCycle is also an authorized distributor of HPLC columns from other suppliers:
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SiliaChrom® Preparative HPLC Columns SB C8
SiliaChrom® SB C18 has the particularity that the silane carries two different functions:
• The C18 Hydrophobic function
• Transversal hindered isopropyl groups which protect the surface from an acidic attack.
This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)
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SiliaChrom® Preparative Guard Cartridge XT C18 Fidelity
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.
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SiliaChrom® Preparative HPLC Columns XT C18
SiliaChrom® XT C18 has a hybrid surface made from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, presents a very high resistance to extreme high pH conditions (up to 12).
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SiliaChrom® Preparative HPLC Columns HILIC
SiliaChrom® HILIC (Hydrophilic Interaction Chromatography) HPLC columns are designed to retain highly polar analytes. SiliaChrom HILIC has a selectivity that is complementary to the one of reversed-phase columns. In fact, it has a higher retention for hydrophilic compounds in HILIC mode. HILIC sorbent is more stable and reproducible than normal phase silica or amino columns. This phase is ideal for MedChem laboratories and approved for SFC applications.
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SiliaChrom® Preparative Guard Cartridge HILIC
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.
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SiliaChrom® Preparative HPLC Columns AQ C8
SiliaChrom® AQ C8 columns use a new generation adsorbent based on high purity spherical silica and unique bonding technology.
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SiliaChrom® Preparative HPLC Columns AQ C18
SiliaChrom® AQ C18 columns use a new generation adsorbent based on high purity spherical silica and unique bonding technology.
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SiliaChrom® Preparative Guard Cartridge AQ C18
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.
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SiliaChrom® Preparative Guard Cartridge dt Si
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.
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SiliaChrom® Preparative HPLC Columns dt C18
SiliaChrom® dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series.
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SiliaChrom® Preparative Guard Cartridge dt C18
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.
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SiliaChrom® Preparative HPLC Columns XDB2 C18
SiliaChrom® XDB2 C18 is grafted on ultrapure spherical silica whose surface was activated to create a high concentration of OH before grafting the C18 function. The residual carbon load is lower than the SiliaChrom XDB1 equivalent.
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SiliaChrom® Preparative Guard Cartridge XDB2 C18
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.
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SiliaChrom® Preparative Guard Cartridge XDB1 Si
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.
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SiliaChrom® Preparative HPLC Columns XDB1 CN
SiliaChrom® XDB1 CN is a versatile stationary phase which can be used in normal phase or reverse phase conditions. This stationary phase is ideal for basic pharmaceuticals, steroids and other basic compounds.
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SiliaChrom® Preparative Guard Cartridge XDB1 CN
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.
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SiliaChrom® Preparative Guard Cartridge XDB1 C8
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.
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SiliaChrom® Preparative Guard Cartridge XDB1
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.
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SiliaChrom® Preparative Guard Cartridge SB C8
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.
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SiliaChrom® Preparative HPLC Columns SB C18
SiliaChrom® SB C18 has the particularity that the silane carries two different functions:
• The C18 Hydrophobic function
• Transversal hindered isopropyl groups which protect the surface from an acidic attack.
This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)
-
SiliaChrom® Preparative Guard Cartridge SB C18
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.
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SiliaChrom® Preparative HPLC Columns XDB1 C18
SiliaChrom® XDB1 C18 column has the highest loading density available as well as the highest hydrophobicity. This allows it to have the least interactions between the analytes and potential residual silanol groups.
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SiliaChrom® Preparative HPLC Columns XDB1 C8
SiliaChrom® XDB 1 C8 columns offer faster separations for strongly hydrophobic analytes compared to C18 columns. As the C18 XDB1 phase, the C8 XDB1 is highly base deactivated.
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SiliaChrom® Preparative HPLC Columns dt Si
SiliaChrom® dt Si has the same unique technology phase as SiliaChrom AQ Si, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series. This phase has no metal content, in order to avoid secondary interaction with basic or ion species.
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SiliaChrom® Preparative HPLC Columns XDB1 AMINO
SiliaChrom® XDB1 NH2 is a very versatile stationary phase which can be used in normal phase, reverse phase or ion exchange conditions as a weak anionic exchanger (WAX).
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SiliaChrom® Preparative HPLC Columns XDB1 Si
SiliaChrom® XDB1 Si is ideal for normal phase applications. This spherical silica support is characterized by its very high level of purity (99.999 %).
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SiliaChrom® Preparative Guard Cartridge AQ C8
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.
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SiliaChrom® Preparative Guard Cartridge XT C18
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.
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SiliaChrom® Preparative HPLC Columns XT C18 Fidelity
SiliaChrom® XT Fidelity C18 has a hybrid surface composed from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, has an incomparable resistance to extreme high pH conditions (up to 12). The only difference between SiliaChrom XT C18 and SiliaChrom XT Fidelity C18 is the way they are packed, giving Fidelity columns more robustness at high pH and temperature.
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SiliaChrom® Preparative HPLC Columns SB C18-300
SiliaChrom® C18 reverse phase functionalization is completely identical to the SB C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
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SiliaChrom® Preparative HPLC Columns SB C8-300
SiliaChrom® C8 reverse phase functionalization is completely identical to the SB C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
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SiliaChrom® Preparative HPLC Columns XDB1 C18-300
SiliaChrom® XDB1 C18-300 reverse phase functionalization is completely identical to the XDB1 C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
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SiliaChrom® Preparative HPLC Columns XDB1 C8-300
SiliaChrom® XDB1 C8-300 reverse phase functionalization is completely identical to the XDB1 C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
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SiliaChrom® Preparative HPLC Columns XDB1 CN-300
SiliaChrom® XDB1 CN-300 chromatography phase functionalization is completely identical to the XDB1 CN equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
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SiliaChrom® Preparative HPLC Columns XDB1 Si-300
SiliaChrom® XDB1 Si-300 is an ultrapure spherical silica support with a pore size of 300 Å. This spherical silica support is characterized by its very high level of purity (99.999 %).
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SiliaChrom® Preparative HPLC Columns XDB1 PHENYL-300
SiliaChrom® XDB1 Phenyl-300 chromatography phase functionalization is completely identical to the XDB1 Phenyl equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
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SiliaChrom® Preparative HPLC Columns XDB1 AMINO-300
SiliaChrom® Amino chromatographic phase functionalization is completely identical to the XDB1 AMINO equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
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SiliaChrom® Preparative HPLC Columns XDB1 C4-300
SiliaChrom® XDB1 C4-300 reverse phase functionalization is completely identical to the XDB1 C4 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
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SiliaChrom® Preparative HPLC Columns XDB1 C1-300
SiliaChrom® XDB1 C1-300 reverse phase functionalization is completely identical to the XDB1 C1 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
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SiliaChrom® Preparative HPLC Columns HILIC-300
The chromatographic HILIC function is grafted on a 300 Å pore size spherical silica to separate or purify larger compounds.
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SiliaChrom® Preparative HPLC Columns RPC-H
This polymer based stationary phase presents a high C18 substitution. You will enjoy it in extreme pH conditions for your reverse phase applications.
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SiliaChrom® Preparative HPLC Columns RPC-M
This polymer based stationary phase presents a lower C18 substitution than the SiliaChrom® RPC-M stationary phase. You will enjoy it in extreme pH conditions for your reverse phase applications.
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SiliaChrom® Analytical Guard Cartridge dt C18
The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.







