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SiliaChrom®- HPLC Columns

SiliCycle manufactures a variety of HPLC columns for reversed and normal phases applications. The SiliaChrom series contain more than 20 different phases produced in our own laboratories, and we continue to develop additional unique and powerful HPLC columns. In fact, you can be completely assured of the quality, from raw material synthesis through packing, testing and customer applications.

SiliaChrom HPLC columns are made of highly pure spherical particles. One of the most remarkable properties of every SiliaChrom column is that all types of organic compounds (acidic, basic and neutral) will have optimal peak symmetry with excellent efficiency across the whole applicable pH range. Lot-to-lot reproducibility is ensured by a stable bonding and endcapping process. All columns are packed using a consistent slurry packing process to achieve uniform and stable bed for maximum column efficiency, long lifetime and column-to-column reproducibility.

SiliaChrom HPLC Columns

Main characteristics  > Benefits for the column users
Broad pH range (0,8-12,0) Flexibility for method development
Compatible with 100% aqueous mobile phase Maximum versatility
High surface coverage Tolerance to large volume and mass injected
Minimum buffer concentration needed Better sensitivity for LC-MS and column lifetime
Low bleed High sensitivity for LC-MS
Wide variety of chemistries available Broad selection of polarity
Excellent column effiency Better and faster separation
Long column lifetime Lower cost
Thightest specification Better confidence in HPLC results

 

Standard HPLC Columns

SiliaChrom HPLC columns are available in Narrow Bore, Analytical, Semi-Preparative, and Preparative formats.

SiliaChrom HPLC Standard Column Dimensions
  SiliaChrom HPLC Column Length (mm)
20
30
50
100
150
200
250
Particle Size (µm)
2.5, 3 & 5
2.5, 3 & 5
2.5, 3, 5, 7 & 10
2.5, 3, 5, 7, 10 & 20
2.5, 3, 5, 7, 10 & 20
5 & 10
2.5, 3, 5, 7, 10 & 20
SiliaChrom
HPLC Column
Internal Diameter (mm)		 2.0
S
S
S
S
S
C
C
2.1
S
S
S
S
S
C
C
3.0
C
S
S
S
S
C
C
4.6
C
S
S
S
S
S
S
10
C
C
C
S
S
S
S
20
C
C
C
C
S
S
S
30
C
C
C
S
S
S
S
50
C
C
C
S
S
S
S
100
C
C
C
C
C
C
C
  S = Standard C = Custom

 

Distribution

SiliCycle is also an authorized distributor of HPLC columns from other suppliers:

Nacalai Tesque inc.
nacalai tesque inc. - Specialized HPLC Columns Sugar-D, Cholester, HILIC, π-NAP		 SIELC Technologies
SIELC Technologies - Mixed Mode Stationary Phases HPLC Columns

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  1. SiliaChrom® Preparative Guard Cartridge XT C18 Fidelity

    SiliaChrom® Preparative Guard Cartridge XT C18 Fidelity

    The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.

  2. SiliaChrom® Preparative Guard Cartridge HILIC

    SiliaChrom® Preparative Guard Cartridge HILIC

    The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.

  3. SiliaChrom® Preparative Guard Cartridge AQ C18

    SiliaChrom® Preparative Guard Cartridge AQ C18

    The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.

  4. SiliaChrom® Preparative Guard Cartridge dt Si

    SiliaChrom® Preparative Guard Cartridge dt Si

    The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.

  5. SiliaChrom® Preparative Guard Cartridge dt C18

    SiliaChrom® Preparative Guard Cartridge dt C18

    The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.

  6. SiliaChrom® Preparative Guard Cartridge XDB2 C18

    SiliaChrom® Preparative Guard Cartridge XDB2 C18

    The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.

  7. SiliaChrom® Preparative Guard Cartridge XDB1 Si

    SiliaChrom® Preparative Guard Cartridge XDB1 Si

    The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.

  8. SiliaChrom® Preparative Guard Cartridge XDB1 C8

    SiliaChrom® Preparative Guard Cartridge XDB1 C8

    The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.

  9. SiliaChrom® Preparative Guard Cartridge XDB1

    SiliaChrom® Preparative Guard Cartridge XDB1

    The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.

  10. SiliaChrom® Preparative Guard Cartridge AQ C8

    SiliaChrom® Preparative Guard Cartridge AQ C8

    The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.

  11. SiliaChrom® Preparative Guard Cartridge XT C18

    SiliaChrom® Preparative Guard Cartridge XT C18

    The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.

  12. SiliaChrom® Narrow Bore HPLC Columns SB C18

    SiliaChrom® Narrow Bore HPLC Columns SB C18

    SiliaChrom® SB C18 has the particularity that the silane carries two different functions:

    • The C18 Hydrophobic function

    • Transversal hindered isopropyl groups which protect the surface from an acidic attack.

    This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)

  13. SiliaChrom® Narrow Bore HPLC Columns SB C8

    SiliaChrom® Narrow Bore HPLC Columns SB C8

    SiliaChrom® SB C18 has the particularity that the silane carries two different functions:

    • The C18 Hydrophobic function

    • Transversal hindered isopropyl groups which protect the surface from an acidic attack.

    This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)

  14. SiliaChrom® Narrow Bore HPLC Columns XDB1 C18

    SiliaChrom® Narrow Bore HPLC Columns XDB1 C18

    SiliaChrom® XDB1 C18 column has the highest loading density available as well as the highest hydrophobicity. This allows it to have the least interactions between the analytes and potential residual silanol groups.

  15. SiliaChrom® Narrow Bore HPLC Columns XDB1 C8

    SiliaChrom® Narrow Bore HPLC Columns XDB1 C8

    SiliaChrom® XDB 1 C8 columns offer faster separations for strongly hydrophobic analytes compared to C18 columns. As the C18 XDB1 phase, the C8 XDB1 is highly base deactivated.

  16. SiliaChrom® Narrow Bore HPLC Columns XDB1 CN

    SiliaChrom® Narrow Bore HPLC Columns XDB1 CN

    SiliaChrom® XDB1 CN is a versatile stationary phase which can be used in normal phase or reverse phase conditions. This stationary phase is ideal for basic pharmaceuticals, steroids and other basic compounds.

  17. SiliaChrom® Narrow Bore HPLC Columns XDB1 PHENYL

    SiliaChrom® Narrow Bore HPLC Columns XDB1 PHENYL

    SiliaChrom® XDB1 Phenyl can conjugate hydrophobic interactions and electronic π-π interactions with unsaturated compounds. This stationary phase is excellent for polynuclear aromatic hydrocarbons, purines and polar aromatics.

  18. SiliaChrom® Narrow Bore HPLC Columns XDB1 C4

    SiliaChrom® Narrow Bore HPLC Columns XDB1 C4

    SiliaChrom® XDB1 C4 presents a less hydrophobic character than C18 and C8 SiliaChrom XDB1 columns. This stationary phase is ideal for peptides and small proteins.

  19. SiliaChrom® Narrow Bore HPLC Columns XDB1 C1

    SiliaChrom® Narrow Bore HPLC Columns XDB1 C1

    SiliaChrom®XDB1 C1 is the least hydrophobic reverse phase available. This phase is useful for very high hydrophobic molecules.

  20. SiliaChrom® Narrow Bore HPLC Columns XDB2 C18

    SiliaChrom® Narrow Bore HPLC Columns XDB2 C18

    SiliaChrom® XDB2 C18 is grafted on ultrapure spherical silica whose surface was activated to create a high concentration of OH before grafting the C18 function. The residual carbon load is lower than the SiliaChrom XDB1 equivalent.

  21. SiliaChrom® Narrow Bore HPLC Columns dt C18

    SiliaChrom® Narrow Bore HPLC Columns dt C18

    SiliaChrom® dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series. 

  22. SiliaChrom® Narrow Bore HPLC Columns AQ C18

    SiliaChrom® Narrow Bore HPLC Columns AQ C18

    SiliaChrom® AQ C18 columns use a new generation adsorbent based on high purity spherical silica and unique bonding technology. 

  23. SiliaChrom® Narrow Bore HPLC Columns AQ C8

    SiliaChrom® Narrow Bore HPLC Columns AQ C8

    SiliaChrom® AQ C8 columns use a new generation adsorbent based on high purity spherical silica and unique bonding technology.

  24. SiliaChrom® Narrow Bore HPLC Columns XT C18

    SiliaChrom® Narrow Bore HPLC Columns XT C18

    SiliaChrom® XT C18 has a hybrid surface made from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, presents a very high resistance to extreme high pH conditions (up to 12).

  25. SiliaChrom® Narrow Bore HPLC Columns XT C18 Fidelity

    SiliaChrom® Narrow Bore HPLC Columns XT C18 Fidelity

    SiliaChrom® XT Fidelity C18 has a hybrid surface composed from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, has an incomparable resistance to extreme high pH conditions (up to 12). The only difference between SiliaChrom XT C18 and SiliaChrom XT Fidelity C18 is the way they are packed, giving Fidelity columns more robustness at high pH and temperature.

  26. SiliaChrom® Analytical Guard Cartridge dt C18

    SiliaChrom® Analytical Guard Cartridge dt C18

    The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.

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Application Fields
  1. Bio-Pharma (8)
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Type of Column
  1. Narrow Bore Columns (14)
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  1. L1 (7)
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