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SiliaChrom®- HPLC Columns

SiliCycle manufactures a variety of HPLC columns for reversed and normal phases applications. The SiliaChrom series contain more than 20 different phases produced in our own laboratories, and we continue to develop additional unique and powerful HPLC columns. In fact, you can be completely assured of the quality, from raw material synthesis through packing, testing and customer applications.

SiliaChrom HPLC columns are made of highly pure spherical particles. One of the most remarkable properties of every SiliaChrom column is that all types of organic compounds (acidic, basic and neutral) will have optimal peak symmetry with excellent efficiency across the whole applicable pH range. Lot-to-lot reproducibility is ensured by a stable bonding and endcapping process. All columns are packed using a consistent slurry packing process to achieve uniform and stable bed for maximum column efficiency, long lifetime and column-to-column reproducibility.

SiliaChrom HPLC Columns

Main characteristics  > Benefits for the column users
Broad pH range (0,8-12,0) Flexibility for method development
Compatible with 100% aqueous mobile phase Maximum versatility
High surface coverage Tolerance to large volume and mass injected
Minimum buffer concentration needed Better sensitivity for LC-MS and column lifetime
Low bleed High sensitivity for LC-MS
Wide variety of chemistries available Broad selection of polarity
Excellent column effiency Better and faster separation
Long column lifetime Lower cost
Thightest specification Better confidence in HPLC results

 

Standard HPLC Columns

SiliaChrom HPLC columns are available in Narrow Bore, Analytical, Semi-Preparative, and Preparative formats.

SiliaChrom HPLC Standard Column Dimensions
  SiliaChrom HPLC Column Length (mm)
20
30
50
100
150
200
250
Particle Size (µm)
2.5, 3 & 5
2.5, 3 & 5
2.5, 3, 5, 7 & 10
2.5, 3, 5, 7, 10 & 20
2.5, 3, 5, 7, 10 & 20
5 & 10
2.5, 3, 5, 7, 10 & 20
SiliaChrom
HPLC Column
Internal Diameter (mm)		 2.0
S
S
S
S
S
C
C
2.1
S
S
S
S
S
C
C
3.0
C
S
S
S
S
C
C
4.6
C
S
S
S
S
S
S
10
C
C
C
S
S
S
S
20
C
C
C
C
S
S
S
30
C
C
C
S
S
S
S
50
C
C
C
S
S
S
S
100
C
C
C
C
C
C
C
  S = Standard C = Custom

 

Distribution

SiliCycle is also an authorized distributor of HPLC columns from other suppliers:

Nacalai Tesque inc.
nacalai tesque inc. - Specialized HPLC Columns Sugar-D, Cholester, HILIC, π-NAP		 SIELC Technologies
SIELC Technologies - Mixed Mode Stationary Phases HPLC Columns

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  1. SiliaChrom® Analytical HPLC Columns SB C18-300

    SiliaChrom® Analytical HPLC Columns SB C18-300

    SiliaChrom® C18 reverse phase functionalization is completely identical to the SB C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  2. SiliaChrom® Semi-Preparative HPLC Columns GF-300 AMIDE

    SiliaChrom® Semi-Preparative HPLC Columns GF-300 AMIDE

    This Amide stationary phase, grafted on a silica matrix, is useful for your size exclusion chromatography. SiliaChrom® GF-300 columns present a pore size of 300 Å which permits to separate molecules with a MV between 3 000 and 50 000 Dalton
  3. SiliaChrom® Semi-Preparative HPLC Columns GF-300

    SiliaChrom® Semi-Preparative HPLC Columns GF-300

    This silica based stationary phase is useful for your size exclusion chromatography. SiliaChrom® GF-300 columns present a pore size of 300 Å which permits to separate molecules with a MV between 3 000 and 50 000 Dalton
  4. SiliaChrom® Semi-Preparative HPLC Columns HILIC-300

    SiliaChrom® Semi-Preparative HPLC Columns HILIC-300

    The chromatographic HILIC function is grafted on a 300 Å pore size spherical silica to separate or purify larger compounds.

  5. SiliaChrom® Semi-Preparative HPLC Columns XDB1 C1-300

    SiliaChrom® Semi-Preparative HPLC Columns XDB1 C1-300

    SiliaChrom® XDB1 C1-300 reverse phase functionalization is completely identical to the XDB1 C1 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  6. SiliaChrom® Semi-Preparative HPLC Columns XDB1 C4-300

    SiliaChrom® Semi-Preparative HPLC Columns XDB1 C4-300

    SiliaChrom® XDB1 C4-300 reverse phase functionalization is completely identical to the XDB1 C4 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  7. SiliaChrom® Semi-Preparative HPLC Columns XDB1 AMINO-300

    SiliaChrom® Semi-Preparative HPLC Columns XDB1 AMINO-300

    SiliaChrom® Amino chromatographic phase functionalization is completely identical to the XDB1 AMINO equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  8. SiliaChrom® Semi-Preparative HPLC Columns XDB1 PHENYL-300

    SiliaChrom® Semi-Preparative HPLC Columns XDB1 PHENYL-300

    SiliaChrom® XDB1 Phenyl-300 chromatography phase functionalization is completely identical to the XDB1 Phenyl equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  9. SiliaChrom® Semi-Preparative HPLC Columns XDB1 Si-300

    SiliaChrom® Semi-Preparative HPLC Columns XDB1 Si-300

    SiliaChrom® XDB1 Si-300 is an ultrapure spherical silica support with a pore size of 300 Å. This spherical silica support is characterized by its very high level of purity (99.999 %).

  10. SiliaChrom® Semi-Preparative HPLC Columns XDB1 CN-300

    SiliaChrom® Semi-Preparative HPLC Columns XDB1 CN-300

    SiliaChrom® XDB1 CN-300 chromatography phase functionalization is completely identical to the XDB1 CN equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  11. SiliaChrom® Semi-Preparative HPLC Columns XDB1 C8-300

    SiliaChrom® Semi-Preparative HPLC Columns XDB1 C8-300

    The SiliaChrom® XDB1 C8-300 reverse phase functionalization is completely identical to the XDB1 C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  12. SiliaChrom® Semi-Preparative HPLC Columns XDB1 C18-300

    SiliaChrom® Semi-Preparative HPLC Columns XDB1 C18-300

    SiliaChrom® XDB1 C18-300 reverse phase functionalization is completely identical to the XDB1 C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  13. SiliaChrom® Semi-Preparative HPLC Columns SB C8-300

    SiliaChrom® Semi-Preparative HPLC Columns SB C8-300

    SiliaChrom® C8 reverse phase functionalization is completely identical to the SB C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  14. SiliaChrom® Semi-Preparative HPLC Columns SB C18-300

    SiliaChrom® Semi-Preparative HPLC Columns SB C18-300

    SiliaChrom® C18 reverse phase functionalization is completely identical to the SB C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  15. SiliaChrom® Preparative HPLC Columns SB C18-300

    SiliaChrom® Preparative HPLC Columns SB C18-300

    SiliaChrom® C18 reverse phase functionalization is completely identical to the SB C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  16. SiliaChrom® Preparative HPLC Columns SB C8-300

    SiliaChrom® Preparative HPLC Columns SB C8-300

    SiliaChrom® C8 reverse phase functionalization is completely identical to the SB C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  17. SiliaChrom® Preparative HPLC Columns XDB1 C18-300

    SiliaChrom® Preparative HPLC Columns XDB1 C18-300

    SiliaChrom® XDB1 C18-300 reverse phase functionalization is completely identical to the XDB1 C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  18. SiliaChrom® Preparative HPLC Columns XDB1 C8-300

    SiliaChrom® Preparative HPLC Columns XDB1 C8-300

    SiliaChrom® XDB1 C8-300 reverse phase functionalization is completely identical to the XDB1 C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  19. SiliaChrom® Preparative HPLC Columns XDB1 CN-300

    SiliaChrom® Preparative HPLC Columns XDB1 CN-300

    SiliaChrom® XDB1 CN-300 chromatography phase functionalization is completely identical to the XDB1 CN equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  20. SiliaChrom® Preparative HPLC Columns XDB1 Si-300

    SiliaChrom® Preparative HPLC Columns XDB1 Si-300

    SiliaChrom® XDB1 Si-300 is an ultrapure spherical silica support with a pore size of 300 Å. This spherical silica support is characterized by its very high level of purity (99.999 %).

  21. SiliaChrom® Preparative HPLC Columns XDB1 PHENYL-300

    SiliaChrom® Preparative HPLC Columns XDB1 PHENYL-300

    SiliaChrom® XDB1 Phenyl-300 chromatography phase functionalization is completely identical to the XDB1 Phenyl equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  22. SiliaChrom® Preparative HPLC Columns XDB1 AMINO-300

    SiliaChrom® Preparative HPLC Columns XDB1 AMINO-300

    SiliaChrom® Amino chromatographic phase functionalization is completely identical to the XDB1 AMINO equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  23. SiliaChrom® Preparative HPLC Columns XDB1 C4-300

    SiliaChrom® Preparative HPLC Columns XDB1 C4-300

    SiliaChrom® XDB1 C4-300 reverse phase functionalization is completely identical to the XDB1 C4 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  24. SiliaChrom® Preparative HPLC Columns XDB1 C1-300

    SiliaChrom® Preparative HPLC Columns XDB1 C1-300

    SiliaChrom® XDB1 C1-300 reverse phase functionalization is completely identical to the XDB1 C1 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  25. SiliaChrom® Preparative HPLC Columns HILIC-300

    SiliaChrom® Preparative HPLC Columns HILIC-300

    The chromatographic HILIC function is grafted on a 300 Å pore size spherical silica to separate or purify larger compounds.

  26. SiliaChrom® Narrow Bore HPLC Columns C18/SCX

    SiliaChrom® Narrow Bore HPLC Columns C18/SCX

    Separate ionic and hydrophobic compounds at the same time. No more need for pairing agent or using columns in series. Gain in efficiency and time by using mixed mode SiliaChrom® HPLC columns.

  27. SiliaChrom® Narrow Bore HPLC Columns SB C18-300

    SiliaChrom® Narrow Bore HPLC Columns SB C18-300

    SiliaChrom® C18 reverse phase functionalization is completely identical to the SB C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  28. SiliaChrom® Narrow Bore HPLC Columns SB C8-300

    SiliaChrom® Narrow Bore HPLC Columns SB C8-300

    SiliaChrom® C8 reverse phase functionalization is completely identical to the SB C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  29. SiliaChrom® Narrow Bore HPLC Columns XDB1 C18-300

    SiliaChrom® Narrow Bore HPLC Columns XDB1 C18-300

    SiliaChrom® XDB1 C18-300 reverse phase functionalization is completely identical to the XDB1 C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  30. SiliaChrom® Narrow Bore HPLC Columns XDB1 C8-300

    SiliaChrom® Narrow Bore HPLC Columns XDB1 C8-300

    SiliaChrom® XDB1 C8-300 reverse phase functionalization is completely identical to the XDB1 C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  31. SiliaChrom® Narrow Bore HPLC Columns XDB1 CN-300

    SiliaChrom® Narrow Bore HPLC Columns XDB1 CN-300

    SiliaChrom® XDB1 CN-300 chromatography phase functionalization is completely identical to the XDB1 CN equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  32. SiliaChrom® Narrow Bore HPLC Columns XDB1 Si-300

    SiliaChrom® Narrow Bore HPLC Columns XDB1 Si-300

    SiliaChrom® XDB1 Si-300 is an ultrapure spherical silica support with a pore size of 300 Å. This spherical silica support is characterized by its very high level of purity (99.999 %).

  33. SiliaChrom® Narrow Bore HPLC Columns XDB1 PHENYL-300

    SiliaChrom® Narrow Bore HPLC Columns XDB1 PHENYL-300

    SiliaChrom® XDB1 Phenyl-300 chromatography phase functionalization is completely identical to the XDB1 Phenyl equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  34. SiliaChrom® Narrow Bore HPLC Columns XDB1 DIOL-300

    SiliaChrom® Narrow Bore HPLC Columns XDB1 DIOL-300

    SiliaChrom® XDB1 DIOL-300 chromatography phase functionalization is completely identical to the XDB1 DIOL equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  35. SiliaChrom® Narrow Bore HPLC Columns XDB1 AMINO-300

    SiliaChrom® Narrow Bore HPLC Columns XDB1 AMINO-300

    SiliaChrom® Amino chromatographic phase functionalization is completely identical to the XDB1 AMINO equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  36. SiliaChrom® Narrow Bore HPLC Columns XDB1 C4-300

    SiliaChrom® Narrow Bore HPLC Columns XDB1 C4-300

    SiliaChrom® XDB1 C4-300 reverse phase functionalization is completely identical to the XDB1 C4 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  37. SiliaChrom® Narrow Bore HPLC Columns XDB1 C1-300

    SiliaChrom® Narrow Bore HPLC Columns XDB1 C1-300

    SiliaChrom® XDB1 C1-300 reverse phase functionalization is completely identical to the XDB1 C1 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  38. SiliaChrom® Narrow Bore HPLC Columns HILIC-300

    SiliaChrom® Narrow Bore HPLC Columns HILIC-300

    The chromatographic HILIC function is grafted on a 300 Å pore size spherical silica to separate or purify larger compounds.

  39. SiliaChrom® Narrow Bore HPLC Columns SCX-300

    SiliaChrom® Narrow Bore HPLC Columns SCX-300

    The strong cationic exchange function is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  40. SiliaChrom® Narrow Bore HPLC Columns SAX-300

    SiliaChrom® Narrow Bore HPLC Columns SAX-300

    The strong anionic exchanger function is grafted on a 300 Å pore size spherical silica to separate or purify larger compounds.

  41. SiliaChrom® Analytical Guard Cartridge C18/SCX

    SiliaChrom® Analytical Guard Cartridge C18/SCX

    The SiliaChrom Guard Cartridges increase the shelf life of the SiliaChrom HPLC Columns by retaining impurities before the analytical columns.

  42. SiliaChrom® Analytical HPLC Columns C18/SCX

    SiliaChrom® Analytical HPLC Columns C18/SCX

    Separate ionic and hydrophobic compounds at the same time. No more need for pairing agents or for the use of columns in series. Gain in efficiency and time by using mixed mode SiliaChrom® HPLC columns.

  43. SiliaChrom® Analytical HPLC Columns SB C8-300

    SiliaChrom® Analytical HPLC Columns SB C8-300

    SiliaChrom® C8 reverse phase functionalization is completely identical to the SB C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  44. SiliaChrom® Analytical HPLC Columns XDB1 C18-300

    SiliaChrom® Analytical HPLC Columns XDB1 C18-300

    SiliaChrom® XDB1 C18-300 reverse phase functionalization is completely identical to the XDB1 C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  45. SiliaChrom® Analytical HPLC Columns XDB1 C8-300

    SiliaChrom® Analytical HPLC Columns XDB1 C8-300

    SiliaChrom® XDB1 C8-300 reverse phase functionalization is completely identical to the XDB1 C8 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  46. SiliaChrom® Analytical HPLC Columns XDB1 CN-300

    SiliaChrom® Analytical HPLC Columns XDB1 CN-300

    SiliaChrom® XDB1 CN-300 chromatography phase functionalization is completely identical to the XDB1 CN equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  47. SiliaChrom® Analytical HPLC Columns XDB1 Si-300

    SiliaChrom® Analytical HPLC Columns XDB1 Si-300

    SiliaChrom® XDB1 Si-300 is an ultrapure spherical silica support with a pore size of 300 Å. This spherical silica support is characterized by its very high level of purity (99.999 %).

  48. SiliaChrom® Analytical HPLC Columns XDB1 PHENYL-300

    SiliaChrom® Analytical HPLC Columns XDB1 PHENYL-300

    SiliaChrom® XDB1 Phenyl-300 chromatography phase functionalization is completely identical to the XDB1 Phenyl equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  49. SiliaChrom® Analytical HPLC Columns XDB1 DIOL-300

    SiliaChrom® Analytical HPLC Columns XDB1 DIOL-300

    SiliaChrom® XDB1 DIOL-300 chromatography phase functionalization is completely identical to the XDB1 DIOL equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

  50. SiliaChrom® Analytical HPLC Columns XDB1 AMINO-300

    SiliaChrom® Analytical HPLC Columns XDB1 AMINO-300

    SiliaChrom® Amino chromatographic phase functionalization is completely identical to the XDB1 AMINO equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.

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