
SiliaChrom®- HPLC Columns
SiliCycle manufactures a variety of HPLC columns for reversed and normal phases applications. The SiliaChrom series contain more than 20 different phases produced in our own laboratories, and we continue to develop additional unique and powerful HPLC columns. In fact, you can be completely assured of the quality, from raw material synthesis through packing, testing and customer applications.
SiliaChrom HPLC columns are made of highly pure spherical particles. One of the most remarkable properties of every SiliaChrom column is that all types of organic compounds (acidic, basic and neutral) will have optimal peak symmetry with excellent efficiency across the whole applicable pH range. Lot-to-lot reproducibility is ensured by a stable bonding and endcapping process. All columns are packed using a consistent slurry packing process to achieve uniform and stable bed for maximum column efficiency, long lifetime and column-to-column reproducibility.
SiliaChrom HPLC Columns
| Main characteristics > | Benefits for the column users |
|---|---|
| Broad pH range (0,8-12,0) | Flexibility for method development |
| Compatible with 100% aqueous mobile phase | Maximum versatility |
| High surface coverage | Tolerance to large volume and mass injected |
| Minimum buffer concentration needed | Better sensitivity for LC-MS and column lifetime |
| Low bleed | High sensitivity for LC-MS |
| Wide variety of chemistries available | Broad selection of polarity |
| Excellent column effiency | Better and faster separation |
| Long column lifetime | Lower cost |
| Thightest specification | Better confidence in HPLC results |
Standard HPLC Columns
SiliaChrom HPLC columns are available in Narrow Bore, Analytical, Semi-Preparative, and Preparative formats.
| SiliaChrom HPLC Standard Column Dimensions | ||||||||
|---|---|---|---|---|---|---|---|---|
| SiliaChrom HPLC Column Length (mm) | ||||||||
20 |
30 |
50 |
100 |
150 |
200 |
250 |
||
| Particle Size (µm) | 2.5, 3 & 5 |
2.5, 3 & 5 |
2.5, 3, 5, 7 & 10 |
2.5, 3, 5, 7, 10 & 20 |
2.5, 3, 5, 7, 10 & 20 |
5 & 10 |
2.5, 3, 5, 7, 10 & 20 |
|
| SiliaChrom HPLC Column Internal Diameter (mm) |
2.0 | S |
S |
S |
S |
S |
C |
C |
| 2.1 | S |
S |
S |
S |
S |
C |
C |
|
| 3.0 | C |
S |
S |
S |
S |
C |
C |
|
| 4.6 | C |
S |
S |
S |
S |
S |
S |
|
| 10 | C |
C |
C |
S |
S |
S |
S |
|
| 20 | C |
C |
C |
C |
S |
S |
S |
|
| 30 | C |
C |
C |
S |
S |
S |
S |
|
| 50 | C |
C |
C |
S |
S |
S |
S |
|
| 100 | C |
C |
C |
C |
C |
C |
C |
|
| S = Standard C = Custom | ||||||||
Distribution
SiliCycle is also an authorized distributor of HPLC columns from other suppliers:
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SiliaChrom® Analytical HPLC Columns SB C18-300
SiliaChrom® C18 reverse phase functionalization is completely identical to the SB C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
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SiliaChrom® Preparative HPLC Columns XT C18
SiliaChrom® XT C18 has a hybrid surface made from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, presents a very high resistance to extreme high pH conditions (up to 12).
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SiliaChrom® Preparative HPLC Columns AQ C18
SiliaChrom® AQ C18 columns use a new generation adsorbent based on high purity spherical silica and unique bonding technology.
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SiliaChrom® Preparative HPLC Columns dt C18
SiliaChrom® dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series.
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SiliaChrom® Preparative HPLC Columns XDB2 C18
SiliaChrom® XDB2 C18 is grafted on ultrapure spherical silica whose surface was activated to create a high concentration of OH before grafting the C18 function. The residual carbon load is lower than the SiliaChrom XDB1 equivalent.
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SiliaChrom® Preparative HPLC Columns SB C18
SiliaChrom® SB C18 has the particularity that the silane carries two different functions:
• The C18 Hydrophobic function
• Transversal hindered isopropyl groups which protect the surface from an acidic attack.
This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)
-
SiliaChrom® Semi-Preparative HPLC Columns XDB1 C18-300
SiliaChrom® XDB1 C18-300 reverse phase functionalization is completely identical to the XDB1 C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
-
SiliaChrom® Semi-Preparative HPLC Columns SB C18-300
SiliaChrom® C18 reverse phase functionalization is completely identical to the SB C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
-
SiliaChrom® Semi-Preparative HPLC Columns XT C18 Fidelity
SiliaChrom® XT Fidelity C18 has a hybrid surface composed from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, has an incomparable resistance to extreme high pH conditions (up to 12). The only difference between SiliaChrom XT C18 and SiliaChrom XT Fidelity C18 is the way they are packed, giving Fidelity columns more robustness at high pH and temperature.
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SiliaChrom® Semi-Preparative HPLC Columns XT C18
SiliaChrom® XT C18 has a hybrid surface made from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, presents a very high resistance to extreme high pH conditions (up to 12).
-
SiliaChrom® Semi-Preparative HPLC Columns AQ C18
SiliaChrom® AQ C18 columns use a new generation adsorbent based on high purity spherical silica and unique bonding technology.
-
SiliaChrom® Semi-Preparative HPLC Columns dt C18
SiliaChrom® dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series.
-
SiliaChrom® Semi-Preparative HPLC Columns XDB2 C18
SiliaChrom® XDB2 C18 is grafted on ultrapure spherical silica whose surface was activated to create a high concentration of OH before grafting the C18 function. The residual carbon load is lower than the SiliaChrom XDB1 equivalent.
-
SiliaChrom® Semi-Preparative HPLC Columns XDB1 C18
SiliaChrom® XDB1 C18 column has the highest loading density available as well as the highest hydrophobicity. This allows it to have the least interactions between the analytes and potential residual silanol groups.
-
SiliaChrom® Semi-Preparative HPLC Columns SB C18
SiliaChrom® SB C18 has the particularity that the silane carries two different functions:
• The C18 Hydrophobic function
• Transversal hindered isopropyl groups which protect the surface from an acidic attack.
This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)
-
SiliaChrom® Preparative HPLC Columns XDB1 C18
SiliaChrom® XDB1 C18 column has the highest loading density available as well as the highest hydrophobicity. This allows it to have the least interactions between the analytes and potential residual silanol groups.
-
SiliaChrom® Preparative HPLC Columns XT C18 Fidelity
SiliaChrom® XT Fidelity C18 has a hybrid surface composed from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, has an incomparable resistance to extreme high pH conditions (up to 12). The only difference between SiliaChrom XT C18 and SiliaChrom XT Fidelity C18 is the way they are packed, giving Fidelity columns more robustness at high pH and temperature.
-
SiliaChrom® Preparative HPLC Columns SB C18-300
SiliaChrom® C18 reverse phase functionalization is completely identical to the SB C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
-
SiliaChrom® Preparative HPLC Columns XDB1 C18-300
SiliaChrom® XDB1 C18-300 reverse phase functionalization is completely identical to the XDB1 C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
-
SiliaChrom® Narrow Bore HPLC Columns SB C18
SiliaChrom® SB C18 has the particularity that the silane carries two different functions:
• The C18 Hydrophobic function
• Transversal hindered isopropyl groups which protect the surface from an acidic attack.
This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)
-
SiliaChrom® Narrow Bore HPLC Columns XDB C18
SiliaChrom® XDB C18 presents the same bonded phase as the SiliaChrom XDB 1 C18 and XDB1 C18-300. The pore size of the silica support is intermediate, 150 Å, which allows to separate larger molecules than its 100 Å equivalent. The lower surface associated allows compounds to have shorter retention times. SiliaChrom XDB C18 is an excellent equivalent to Hypersil BDS-C18 and Zorbax XDB-C18.
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SiliaChrom® Narrow Bore HPLC Columns XDB1 C18
SiliaChrom® XDB1 C18 column has the highest loading density available as well as the highest hydrophobicity. This allows it to have the least interactions between the analytes and potential residual silanol groups.
-
SiliaChrom® Narrow Bore HPLC Columns XDB2 C18
SiliaChrom® XDB2 C18 is grafted on ultrapure spherical silica whose surface was activated to create a high concentration of OH before grafting the C18 function. The residual carbon load is lower than the SiliaChrom XDB1 equivalent.
-
SiliaChrom® Narrow Bore HPLC Columns dt C18
SiliaChrom® dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series.
-
SiliaChrom® Narrow Bore HPLC Columns AQ C18
SiliaChrom® AQ C18 columns use a new generation adsorbent based on high purity spherical silica and unique bonding technology.
-
SiliaChrom® Narrow Bore HPLC Columns XT C18
SiliaChrom® XT C18 has a hybrid surface made from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, presents a very high resistance to extreme high pH conditions (up to 12).
-
SiliaChrom® Narrow Bore HPLC Columns XT C18 Fidelity
SiliaChrom® XT Fidelity C18 has a hybrid surface composed from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, has an incomparable resistance to extreme high pH conditions (up to 12). The only difference between SiliaChrom XT C18 and SiliaChrom XT Fidelity C18 is the way they are packed, giving Fidelity columns more robustness at high pH and temperature.
-
SiliaChrom® Narrow Bore HPLC Columns SB C18-300
SiliaChrom® C18 reverse phase functionalization is completely identical to the SB C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
-
SiliaChrom® Narrow Bore HPLC Columns XDB1 C18-300
SiliaChrom® XDB1 C18-300 reverse phase functionalization is completely identical to the XDB1 C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.
-
SiliaChrom® Analytical HPLC Columns SB C18
SiliaChrom® SB C18 has the particularity that the silane carries two different functions:
• The C18 Hydrophobic function
• Transversal hindered isopropyl groups which protect the surface from an acidic attack.
This chemistry guarantees the stability of this reverse phase in very low pH (up to 0.8)
-
SiliaChrom® Analytical HPLC Columns XDB C18
SiliaChrom® XDB C18 presents the same bonded phase as the SiliaChrom XDB 1 C18 and XDB1 C18-300. The pore size of the silica support is intermediate, 150 Å, which allows to separate larger molecules than its 100 Å equivalent. The lower surface associated allows compounds to have shorter retention times. SiliaChrom XDB C18 is an excellent equivalent to Hypersil BDS-C18 and Zorbax XDB-C18.
-
SiliaChrom® Analytical HPLC Columns XDB1 C18
SiliaChrom® XDB1 C18 column has the highest loading density available as well as the highest hydrophobicity. This allows it to have the least interactions between the analytes and potential residual silanol groups.
-
SiliaChrom® Analytical HPLC Columns XDB2 C18
SiliaChrom® XDB2 C18 is grafted on ultrapure spherical silica whose surface was activated to create a high concentration of OH before grafting the C18 function. The residual carbon load is lower than the SiliaChrom XDB1 equivalent.
-
SiliaChrom® Analytical HPLC Columns dt C18
SiliaChrom® dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series.
-
SiliaChrom® Analytical HPLC Columns AQ C18
SiliaChrom® AQ C18 columns use a new generation adsorbent based on high purity spherical silica and unique bonding technology.
-
SiliaChrom® Analytical HPLC Columns XT C18
SiliaChrom® XT C18 has a hybrid surface made from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, presents a very high resistance to extreme high pH conditions (up to 12).
-
SiliaChrom® Analytical HPLC Columns XT C18 Fidelity
SiliaChrom® XT Fidelity C18 has a hybrid surface composed from a condensation of inorganic SiO2 species and an organosilane (CH3-SiO1.5). This hybrid, a more hydrophobic matrix, has an incomparable resistance to extreme high pH conditions (up to 12). The only difference between SiliaChrom XT C18 and SiliaChrom XT Fidelity C18 is the way they are packed, giving Fidelity columns more robustness at high pH and temperature.
-
SiliaChrom® Analytical HPLC Columns XDB1 C18-300
SiliaChrom® XDB1 C18-300 reverse phase functionalization is completely identical to the XDB1 C18 equivalent. This reverse phase is grafted on a 300 Å pore size spherical silica to separate or purify larger molecules.







