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SiliaSep Accessories

SiliaSep Solid-Load Cartridges

Usually, the use of solid-load technique (also called dry-load) will improve chromatography resolution. SiliaSep Solid-Load luer-lok cartridges are designed to be used with SiliaSep flash cartridges for sample loading.

Either SiliaSep pre-packed (for liquid injection, various choices of media available) or empty solid-load (for silica-sample slurry) are available to better suit your needs.

SiliaSep Solid-Load Cartridges (Luer-Lok)
Product Number Sorbent Weight / Volume Description Qty per box
SPL-R10030B-10U Silica (40 - 63 μm) 2 g / 10 mL SiliaSep Silica Pre-packed Solid-Load Cartridge, 2 g, 10 mL 20
SPL-R10030B-10X Silica (40 - 63 μm) 5 g / 10 mL SiliaSep Silica Pre-packed Solid-Load Cartridge, 5 g, 10 mL 20
SPL-R10030B-60Y Silica (40 - 63 μm) 10 g / 60 mL SiliaSep Silica Pre-packed Solid-Load Cartridge, 10 g, 60 mL 16
SPL-R10030B-60K Silica (40 - 63 μm) 25 g / 60 mL SiliaSep Silica Pre-packed Solid-Load Cartridge, 25 g, 60 mL 16
SPL-R10030B-065 Silica (40 - 63 μm) 65 g / 150 mL SiliaSep Silica Pre-packed XL Solid-Load Cartridge, 65 g, 150 mL 12
SPL-R10030B-270 Silica (40 - 63 μm) 270 g / 700 mL SiliaSep Silica Pre-packed XL Solid-Load Cartridge, 270 g, 700 mL 6
SPL-R52030B-10X Amine 5 g / 10 mL SiliaSep Amine Pre-packed Solid-Load Cartridge, 5 g, 10 mL 20
SPL-R52030B-60K Amine 25 g / 60 mL SiliaSep Amine Pre-packed Solid-Load Cartridge, 25 g, 60 mL 16
SPL-R35030B-10X Diol 5 g / 10 mL SiliaSep Diol Pre-packed Solid-Load Cartridge, 5 g, 10 mL 20
SPL-R35030B-60K Diol 25 g / 60 mL SiliaSep Diol Pre-packed Solid-Load Cartridge, 25 g, 60 mL 16
SPL-R38030B-10X Cyano 5 g / 10 mL SiliaSep Cyano Pre-packed Solid-Load Cartridge, 5 g, 10 mL 20
SPL-R38030B-60K Cyano 25 g / 60 mL SiliaSep Cyano Pre-packed Solid-Load Cartridge, 25 g, 60 mL 16
SPL-R33230B-10X C18 (17%) 5 g / 10 mL SiliaSep C18 (17%) Pre-packed Solid-Load Cartridge, 5 g, 10 mL 20
SPL-R33230B-60K C18 (17%) 25 g / 60 mL SiliaSep C18 (17%) Pre-packed Solid-Load Cartridge, 25 g, 60 mL 16
SPL-0009-010 Empty - / 10 mL SiliaSep Empty Solid-Load Cartridge, 10 mL 100
SPL-0012-060 Empty - / 60 mL SiliaSep Empty Solid-Load Cartridge, 60 mL 100
AUT-0090-150 Empty - / 150 mL SiliaSep Empty Solid-Load Cartridge, 150 mL 12
AUT-0090-700 Empty - / 700 mL SiliaSep Empty Solid-Load Cartridge, 700 mL 6

Note: for optimal purification performance, solvent removal under vacuum is highly recommended.

SiliaSep Plungers

SiliaSep Plungers*
Product Number Description
AUT-0060-010 Plunger for 10 mL solid-load cartridge (16 mm)
AUT-0060-060 Plunger for 60 mL solid-load cartridge (27 mm)

> Download SiliaSep Plungers Operating Instructions Guide

SiliaSep XL Adapter

For SiliaSep XL Flash Cartridges (800g and 1600g), you will need to use our XL Adapter to plug the cartridge on your system.

SiliaSep Accessories for System Conversion

SiliCycle’s SiliaSep Flash Cartridges are designed to be a universal closed-top flash columns (luer-lok connection). Take advantage of SiliaSep’s benefits and enhanced performance by converting your system with an inexpensive adaptor today.

SiliCycle manufactures SiliaSep cartridges that will fit perfectly (no adaptor required) on the following systems:

  • Teledyne Isco CombiFlash® (XL) systems
  • Varian® (Analogix®) IntelliFlash® & SimpliFlash®
  • Interchim PuriFlash 430 evo & Spot II (Armen®)
  • Büchi Sepacore
  • Grace Revelaris

SiliaSep on Biotage Instruments

With Biotage Isolera Systems

With Isolera systems, you can directly use SiliaSep without adapters.

Even if SiliaSep does not have luer-loks at both ends like the SNAP cartridge, you can connect it to your current solvent line (if you prefer to have both ends with luer fittings, contact us for optional adapters).

With Biotage Horizon™, SP1 & SP4 Systems

Note: if you are already using SNAP with your system, no modification is required. See previous point.

With these Biotage’s systems, you have two different options:

Option A: toggle between Biotage and SiliCycle columns

Option B: use only SiliaSep cartridges. No compression module will be necessary from now on.

Option A: Using Adaptors

If you are using the Biotage compression modules (metal cylinders), simply link your existing solvent line connection to the SiliaSep adaptors.

To do so, attach the Biotage Adaptor Kit (PN: KAD-1006) to the existing Swagelock stainless steel connectors, which will allow them to connect to SiliCycle SiliaSep columns. This is only if you want to toggle between Biotage and SiliCycle columns.

Image of an adapter kit

Biotage Adapter Kit (2 pieces)
PN: KAD-1006

Option B: Changing Solvent Lines

Changing the solvent lines from your current system to a Luer-Lok one will allow direct connection of SiliaSep Flash Cartridges. No compression module will be needed from now on.

To switch completely to the SiliaSep columns, simply unscrew the fittings that are currently installed on the Biotage systems and screw in the SiliaSep Solvent Line Replacement (PN: KAD-1014) with the luer connections we supply.

The female luer (A), which connects to the top of the column, goes onto the flow outlet of the instrument (top hole on the instrument).

The male luer (B) fits on top of the slip tip on the bottom of the column and connects to the flow inlet of the instrument (coming out of the column).

Note: SiliaSep Solid-Load Cartridges and Plungers are SiliCycle’s alternative to Biotage’s samplet

SiliaSep Support Rings

Support rings will allow SiliaSep cartridges to sit on the Biotage SP1 & SP4 instruments.

SiliaSep Support Rings
Product Number Description Qty per box
AUT-0068-004 Support ring-4 (16 mm) 5
AUT-0068-010 Support ring-12, 25 (25 mm) 5
AUT-0068-040 Support ring-40 (32 mm) 5
AUT-0068-080 Support ring-80 (36 mm) 5
AUT-0068-120 Support ring-120 (42 mm) 5
KAD-1008 Support ring kit 5 different sizes

Image of the ring

SiliaSep on FlashMaster Instruments

On FlashMaster systems, cartridges are running upside down (reversed). To use SiliaSep cartridges on any FlashMaster instrument, simply connect the SiliaSep cartridge directly on the instrument using the FlashMaster Adapter Kit (PN: KAD-1016) without the plunger. One piece holds the cartridge in place while the other connects to the solvent line.

Image of the adapter