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SiliaPrep Accessories

SiliaPrep- Tips Micro-SPE Cartridges

Using SiliaPrep Tips and Tips Micro-SPE Cartridges guarantees the following benefits:

  • Simple, fast analyte retention & elution with a minimal loss.
  • Sorbents are directly embedded on inner cartridge wall which reduces the risk of contamination.
  • Exceptional binding capacity.
  • No back-pressure.

SiliaPrep Tips for Micro Sample Preparation

SiliaPrep Tips Micro-SPE Cartridges are designed for micro-purification and micro-extraction of femtomole (fmol) to picomole (pmol) quantities of analytes prior to the analysis by mass spectrometric and/or chromatographic techniques. The constant improvement of these techniques of analysis has allowed scientists to decrease the limit of quantification of several applications. This lower limit has pushed SPE manufacturers to design new SPE cartridges accepting small volumes of analytes.

These Tips are specially designed to achieve extraction and purification of small molecules, peptides, phosphopeptides and proteins. SiliaPrep Tips Micro-SPE Cartridges are available in 3 different cartridge formats based on the binding capacity of each embedded sorbent. They are packed with our SiliaBond functionalized silica gels and specialty phases to cover the broadest spectrum of applications requiring small volume of analytes. The SiliaBond phases are embedded directly in the inner surface of the tip to provide consistent flow rates. Finally, no glue has been used during the packing procedure in order to prevent any contamination of the analyte.

Flow Through Channel

SiliaPrep Tips General Experimental Procedure

The following lines present the general experimental procedure for the purification and enrichment of small molecules, peptides and proteins using SiliaPrep Tips Micro-SPE Cartridges.

Conditionning Step:

Attach the SiliaPrep Tips to a micropipette and aspirate/expel 5 times the elution solution and 3 times the binding solution.

A) Loading Step:
Aspirate/expel 20 to 50 times the sample to allow the compounds to adsorb to the sorbent.

B) Washing Step:
Aspirate/expel 10 times the binding solution and discard the expelled solution each time.

C) Elution Step:
Aspirate/expel 10 times the elution solution and collect the expelled solution in a suitable clean tube. Repeat with a fresh portion of elution solution if you want to be sure to collect all of the adsorbed compounds.

 

(Note : repeat 3-5 times for the carbon black sorbent.)

Binding Solution:
0.1% formic acid or 0.05% trifluoroacetic acid (TFA).
Elution Solution:
0.1% formic acid or 0.05% trifluoroacetic acid (TFA) plus ±60% of acetonitrile, propanol or methanol depending on the compound polarities.

Loading Step

Washing Step

Elution Step

 

SiliaPrep Tips Micro-SPE Cartridges Specifications
Tip Volume
(µL)
Sample Volume
(µL)
Binding Capacity
(µg)
Sorbent Weight
(µg)
1 - 10 0.5 - 10 1 30
10 - 200 2 - 25 2.5 75
10 - 200 5 - 50 15 400

22 Item(s)

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  1. SiliaPrep™ Tips C18 Micro-SPE Cartridges

    SiliaPrep™ Tips C18 Micro-SPE Cartridges

    This phase is mainly used for small peptides or protein purification, enrichment or desalting analysis.
  2. SiliaPrep™ Tips C8 Micro-SPE Cartridges

    SiliaPrep™ Tips C8 Micro-SPE Cartridges

    This phase is mainly used for the sample treatment of proteins and peptides requiring a lower hydrophobic capacity.
  3. SiliaPrep™ Tips C4 Micro-SPE Cartridges, 10 µL/30 µg

    SiliaPrep™ Tips C4 Micro-SPE Cartridges, 10 µL/30 µg

    The C4 phase is mainly used for protein purification, enrichment or desalting analysis.
  4. SiliaPrep™ Tips C4 Micro-SPE Cartridges, 200 µL/75 µg

    SiliaPrep™ Tips C4 Micro-SPE Cartridges, 200 µL/75 µg

    The C4 phase is mainly used for protein purification, enrichment or desalting analysis.
  5. SiliaPrep™ Tips C4 Micro-SPE Cartridges, 200 µL/400 µg

    SiliaPrep™ Tips C4 Micro-SPE Cartridges, 200 µL/400 µg

    The C4 phase is mainly used for protein purification, enrichment or desalting analysis.
  6. SiliaPrep™ Tips C4 Micro-SPE Cartridges

    SiliaPrep™ Tips C4 Micro-SPE Cartridges

    The C4 phase is mainly used for protein purification, enrichment or desalting analysis.
  7. SiliaPrep™ Tips Carbon Black Micro-SPE Cartridges, 10 µL/30 µg

    SiliaPrep™ Tips Carbon Black Micro-SPE Cartridges, 10 µL/30 µg

    The CB phase is mainly used for purification of oligosaccharides and other macromolecules containing sugar moities.
  8. SiliaPrep™ Tips Carbon Black Micro-SPE Cartridges, 200 µL/75 µg

    SiliaPrep™ Tips Carbon Black Micro-SPE Cartridges, 200 µL/75 µg

    The CB phase is mainly used for purification of oligosaccharides and other macromolecules containing sugar moities.
  9. SiliaPrep™ Tips Carbon Black Micro-SPE Cartridges, 200 µL/400 µg

    SiliaPrep™ Tips Carbon Black Micro-SPE Cartridges, 200 µL/400 µg

    The CB phase is mainly used for purification of oligosaccharides and other macromolecules containing sugar moities.
  10. SiliaPrep™ Tips Carbon Black Micro-SPE Cartridges

    SiliaPrep™ Tips Carbon Black Micro-SPE Cartridges

    The CB phase is mainly used for purification of oligosaccharides and other macromolecules containing sugar moities.
  11. SiliaPrep™ Tips Mixed-Mode TiO2-ZrO2 Micro-SPE Cartridges, 10 µL/30 µg

    SiliaPrep™ Tips Mixed-Mode TiO2-ZrO2 Micro-SPE Cartridges, 10 µL/30 µg

    The ZrO2-TiO2 sorbent is an excellent alternative for the enrichment of a broad spectrum of phosphopeptides.
  12. SiliaPrep™ Tips Mixed-Mode TiO2-ZrO2 Micro-SPE Cartridges, 200 µL/75 µg

    SiliaPrep™ Tips Mixed-Mode TiO2-ZrO2 Micro-SPE Cartridges, 200 µL/75 µg

    The ZrO2-TiO2 sorbent is an excellent alternative for the enrichment of a broad spectrum of phosphopeptides.
  13. SiliaPrep™ Tips Mixed-Mode TiO2-ZrO2 Micro-SPE Cartridges, 200 µL/400 µg

    SiliaPrep™ Tips Mixed-Mode TiO2-ZrO2 Micro-SPE Cartridges, 200 µL/400 µg

    The ZrO2-TiO2 sorbent is an excellent alternative for the enrichment of a broad spectrum of phosphopeptides.
  14. SiliaPrep™ Tips Mixed-Mode TiO2-ZrO2 Micro-SPE Cartridges

    SiliaPrep™ Tips Mixed-Mode TiO2-ZrO2 Micro-SPE Cartridges

    The ZrO2-TiO2 sorbent is an excellent alternative for the enrichment of a broad spectrum of phosphopeptides.
  15. SiliaPrep™ Tips TiO2 Micro-SPE Cartridges, 10 µL/30 µg

    SiliaPrep™ Tips TiO2 Micro-SPE Cartridges, 10 µL/30 µg

    The TiO2 sorbent is mainly used for phosphopeptide enrichment.
  16. SiliaPrep™ Tips TiO2 Micro-SPE Cartridges, 200 µL/75 µg

    SiliaPrep™ Tips TiO2 Micro-SPE Cartridges, 200 µL/75 µg

    The TiO2 sorbent is mainly used for phosphopeptide enrichment.
  17. SiliaPrep™ Tips TiO2 Micro-SPE Cartridges, 200 µL/400 µg

    SiliaPrep™ Tips TiO2 Micro-SPE Cartridges, 200 µL/400 µg

    The TiO2 sorbent is mainly used for phosphopeptide enrichment.
  18. SiliaPrep™ Tips TiO2 Micro-SPE Cartridges

    SiliaPrep™ Tips TiO2 Micro-SPE Cartridges

    The TiO2 sorbent is mainly used for phosphopeptide enrichment.
  19. SiliaPrep™ Tips ZrO2 Micro-SPE Cartridges, 10 µL/30 µg

    SiliaPrep™ Tips ZrO2 Micro-SPE Cartridges, 10 µL/30 µg

    The ZrO2 sorbent is mainly used for phosphopeptide enrichment
  20. SiliaPrep™ Tips ZrO2 Micro-SPE Cartridges, 200 µL/75 µg

    SiliaPrep™ Tips ZrO2 Micro-SPE Cartridges, 200 µL/75 µg

    The ZrO2 sorbent is mainly used for phosphopeptide enrichment
  21. SiliaPrep™ Tips ZrO2 Micro-SPE Cartridges, 200 µL/400 µg

    SiliaPrep™ Tips ZrO2 Micro-SPE Cartridges, 200 µL/400 µg

    The ZrO2 sorbent is mainly used for phosphopeptide enrichment
  22. SiliaPrep™ Tips ZrO2 Micro-SPE Cartridges

    SiliaPrep™ Tips ZrO2 Micro-SPE Cartridges

    The ZrO2 sorbent is mainly used for phosphopeptide enrichment

22 Item(s)

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