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SiliaQuick QuEChERS for pesticide residue analysis

SiliaQuick QuEChERS

 

Using SiliaQuick QuEChERS ensures the following benefits:

  • Clean extracts from pure products.
  • High recovery and lot-to-lot reproducibility.
  • Great variety of QuEChERS to cover full spectrum of food applications.
  • Reduction of analysis cost.

 

SiliaQuick QuEChERS for Pesticide Residue Analysis

The QuEChERS technique was developed in 2003 by USDA scientists to simplify and accelerate the analysis of pesticides in various fruit and vegetable samples. The name QuEChERS is formed by an acronym of the properties that are observed with this technique: Quick, Easy, Cheap, Effective, Rugged and Safe.

The QuEChERS method has gained in popularity to become the most valuable alternative for determination of traces of analytes in a high throughput environment. Presently, scientists have expanded the use of this method to the analysis of a vast array of pesticides, herbicides, fungicides, antibiotics, drugs, and any other compounds present in all food, beverage, animal and human matrices.

The QuEChERS technique can be summarized as a three-step methodology, starting with a Liquid Extraction, followed by a dispersive Solid-PhaseExtraction clean-up, and completed by a LC or GC Analysis:

  • First step is to carry out the extraction of compounds of interest from your food or beverage matrix through a solvent (mainly acetonitrile).
  • The dispersive Solid-Phase Extraction clean-up is designed to remove specific undesired compounds such as sugars, lipids, organic acids, proteins, pigments and excess water from the solution.
  • Final analysis step consists in a simple injection into a LC or GC coupled with MS or MS/MS instrument to quantify the analyte concentration.
Step 1
Liquid Extraction
Step 2
Dispersive SPE Clean-up
Step 3
LC or GC Analysis
QuEChERS-Step1 Loading Step Loading Step
Extract pesticides or analytes of interest into an organic layer relying on the perfect combination of salts and acetonitrile. Subject organic layer from 1st step to further clean-up and selectively remove unwanted interferences such as lipids and pigments. Analyze your clean from last step andready for GC or HPLC with MS/MS, or your selective detector.

 

SiliaQuick QuEChERS for Food Sample Treatments

SiliaQuick QuEChERS are designed to ensure ultimate performance in pesticide analysis.

Quick: Pre-packed liquid extraction kits and dispersive solid-phase extraction clean-up kits containthe right amount of salts and/or sorbents to suit the specific food matrices, hence eliminating the sample preparation measurement step.

Easy: Preweighed & ready-to-use tubes or packets for only 3 straightforward steps.

Cheap: No specialized equipment or glassware is required to achieve the pesticide residue analysis.

Effective: General procedure for all food and beverage matrices allowing a significant reduction of the analysis cost.

Rugged: Useful for the treatment of complex food matrices such as fish, meat or nuts without the requirement of additional treatments.

Safe: Limited time of contact with dangerous compounds and solvents.

 

Traditional Liquid & Solid Extractions for Sample Preparation involve a number of issues that QuEChERS cam sort out:

Liquid-Phase Extractions are:

  • Labor intensive
  • Necessitate large volumes of organic solvents, including halogenated solvents
  • Difficult to automate
  • Emulsion prone
  • Not specific to a given analyte

Solid-Phase Extractions are:

  • Complex and difficult to master
  • Necessitate large volumes of organic solvents, including halogenated solvents
  • Lengthy to develop: necessitate time & efforts in method development
  • Offer too many possibilities to choose from
  • Ineffective toward many analytes

 

Advantages of QuEChERS over Traditional Sample Preparation

Traditional SPE QuEChERS QuEChERS Benefits
Estimated time to process 6 samples 120 minutes 20 minutes About 6 times faster
Solvent used per sample 90 mL 10 - 15 mL About 6 - 9 times less solvent needed
Chlorinated waste 30 mL None Safer, greener, less costly
Gassware and specialized equipment Clean separatory funnels, waterbath, round bottom flasks, rotary evaporator... Centrifuge No additional supplies needed

Remember, QuEChERS is the only sample preparation technique in line with green chemistry principles and it is highly effective.

 

Extraction and Dispersive Reagents

The following table presents each extraction and dispersive reagent and their specific functions in the QuEChERS technique.

Extraction Reagents Specific Function
Anhydrous Magnesium Sulfate (MgSO4) Facilitates solvent partitioning
Acetic Acid Used for pH adjustment
Acetonitrile Solvent providing the best characteristics for extracting a wide variety of pesticides. Amenable for both LC and GC analysis
Buffers Limits the amount of polar interferences
Sodium Chloride (NaCl) Limits the amount of polar interferences.
Dispersive Reagents Specific Function
SiliaQuick Diamine (Primary Secondary Amine) Removes sugars, fatty acids, organic acids, lipids, and some pigments. Sterols and additional lipids can also be removed in combination with SiliaQuick C18
SiliaQuick Amine Removes sugars and fatty acids as well as the SiliaQuickDiamine but is less likely to catalyze degradation of base sensitive analytes
SiliaQuick C18 Removes long chain, non-polar compounds, and sterols
Graphitized Carbon Black Removes pigments, polyphenols, and other polar compounds
Anhydrous Magnesium Sulfate (MgSO4) Removes residual water from the organic phase

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  1. QE-0001-100P - SiliaQuick™ QuEChERS Liquid Extraction Salt Packet for the Original Method
  2. QE-0002-100P - SiliaQuick™ QuEChERS Liquid Extraction Salt Packet for the AOAC 2007.01 Method
  3. QE-0003-100P- SiliaQuick™ QuEChERS Liquid Extraction Salt Packet for the EN 15662 Method
  4. SiliaQuick™ QuEChERS for General Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 2 mL tube

    SiliaQuick™ QuEChERS for General Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 2 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method,for general fruits & vegetables. 2mL format.
  5. SiliaQuick™ QuEChERS for General Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 2 mL tube

    SiliaQuick™ QuEChERS for General Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 2 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for general fruits & vegetables. 2mL format.
  6. SiliaQuick™ QuEChERS for General Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 15 mL tube

    SiliaQuick™ QuEChERS for General Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 15 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method, for general fruits & vegetables. 15mL format.
  7. SiliaQuick™ QuEChERS for General Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 15 mL tube

    SiliaQuick™ QuEChERS for General Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 15 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for general fruits & vegetables.15mL format.
  8. SiliaQuick™ QuEChERS for Pigmented Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 2 mL tube

    SiliaQuick™ QuEChERS for Pigmented Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 2 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method, for pigmented fruits & vegetables. 2mL format.
  9. SiliaQuick™ QuEChERS for Pigmented Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 2 mL tube

    SiliaQuick™ QuEChERS for Pigmented Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 2 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for pigmented fruits & vegetables. mL format.
  10. SiliaQuick™ QuEChERS for Pigmented Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 15 mL tube

    SiliaQuick™ QuEChERS for Pigmented Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 15 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method, for pigmented fruits & vegetables. 15mL format.
  11. SiliaQuick™ QuEChERS for Pigmented Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 15 mL tube

    SiliaQuick™ QuEChERS for Pigmented Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 15 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for pigmented fruits & vegetables. 15mL format.
  12. SiliaQuick™ QuEChERS for Highly Pigmented and Fatty Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 2 mL tube

    SiliaQuick™ QuEChERS for Highly Pigmented and Fatty Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 2 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method, for highly pigmented and fatty fruits & vegetables. 2mL format.
  13. SiliaQuick™ QuEChERS for Highly Pigmented and Fatty Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 2 mL tube

    SiliaQuick™ QuEChERS for Highly Pigmented and Fatty Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 2 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for highly pigmented and fatty fruits & vegetables. 2mL format.
  14. SiliaQuick™ QuEChERS for Highly Pigmented and Fatty Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 15 mL tube

    SiliaQuick™ QuEChERS for Highly Pigmented and Fatty Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 15 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method, for highly pigmented and fatty fruits & vegetables. 15mL format.
  15. SiliaQuick™ QuEChERS for Highly Pigmented and Fatty Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 15 mL tube

    SiliaQuick™ QuEChERS for Highly Pigmented and Fatty Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 15 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for highly pigmented and fatty fruits & vegetables. 15mL format.
  16. SiliaQuick™ QuEChERS for Fatty & Waxed Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 2 mL tube

    SiliaQuick™ QuEChERS for Fatty & Waxed Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 2 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method, for highly fatty and waxed fruits & vegetables. 2mL format.
  17. SiliaQuick™ QuEChERS for Fatty & Waxed Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 2 mL tube

    SiliaQuick™ QuEChERS for Fatty & Waxed Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 2 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for fatty and waxed fruits & vegetables. 2mL format.
  18. SiliaQuick™ QuEChERS for Fatty & Waxed Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 15 mL tube

    SiliaQuick™ QuEChERS for Fatty & Waxed Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 15 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method, for fatty and waxed fruits & vegetables. 15mL format.
  19. SiliaQuick™ QuEChERS for Fatty & Waxed Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 15 mL tube

    SiliaQuick™ QuEChERS for Fatty & Waxed Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 15 mL tube

    Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for fatty and waxed fruits & vegetables. 15mL format.

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