SiliaChrom® AQ HPLC Columns
SiliaChrom SB columns surfaces were treated with an organic form of silicon to increase the number of silanol groups on the surface. After this step, the surface was bonded with a silane containing two functions. One function is a protecting group that shields the area and protects the surface from an acid attack from the mobile phase: the H3O+ ion does not have access to the surface to break the O-Si
bond (steric effect). The other function is the linear hydrophobic chain with 18 or 8 carbons.