TLC Troubleshooting: Switching To SiliaPlate™ TLC
SiliCycle is manufacturing a wide selection of TLC plates in various sizes (plate size, thickness) and chemistries (C18, NH2, CN, 10% Silver Nitrate). SiliaPlate™ represents an efficient economical alternative to other manufacturers TLC plates while demonstrating high separation power, which is due to the narrow particle size distribution used for manufacturing.
However, switching from one manufacturer’s TLC plates to another can cause small changes in the analytes of interest retention factor (Rf) due to difference in the supporting silica granulometry or activity. Few simple tricks are commonly used in order to quickly and easily reoptimize the elution so that the original TLC pattern can be obtained.
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