HPLC
Most frequently asked questions about high-performance liquid chromatography (HPLC) columns
- What is HPLC?
- HPLC Columns Use Instructions
- What to do when backpressure increases?
- How do I determine the void volume in HPLC?
- Why should I use a guard column with my analytical or preparative column?
- Ideal injection volume vs column dimension
- What happens if my sample solvent is stronger than my mobile phase?
- Can I get a sharper peak by injecting my sample in a weaker injection solvent?
- How much sample can I inject on my LC column?
- What size threads are on the end fittings of my HPLC column?
- Why do most users privilege PEEK vs stainless steel fittings and tubing?
- What is the internal diameter of my LC tubing?
- Can I operate my column with a 100% aqueous mobile phase?
- How can the column performance be checked?
- How can the columns be cleaned or regenerated?
- What is the appropriate flow rate for the different column dimensions?
- What is the difference between dead volume and dwell volume?
- What is the shipping solvent for your HPLC columns?
- When should I use a gradient?
- What are the common buffers used in HPLC?
- Why is my chromatogram showing peak tailing, ghost peaks, fronting peaks, split peaks / shoulder peaks or rounded peaks?
- Why is it difficult to get reproducible retention times in normal phase chromatography?
- What is the difference between a monomeric and polymeric C18 bonded phase?
- How should the columns be stored?
- How to scale-up a column for preparative isolation/purification from analytical conditions?
- Should I run a gradient from 5-95% organic solvent or from 0-100%?
- How are column efficiency, peak asymmetry factor, tailing factor and resolution calculated?
- What to do when there are no peaks, small peaks or negative peaks?
- What to do when there is no flow?
- What is the cause of variable retention times, loss of resolution or selecticity change?
- What is the cause of baseline drift or noise?
Discover our HPLC offer
Our Most Popular Categories
Our New Products
