- > Offre d'emploi: Designer Graphique
- > It’s not just about the shape – Handy guide to (other) common problems in HPLC
- > How to get the perfect shape according to experts – Top 6 most common shape problems in HPLC
- > Some People Excel at Scaling Up Their Purifications and Some Don’t, Which One Are You?
- > Loading in Progress – Avoiding loading capacity mistakes
How do I determine the void volume in HPLC?
The void volume of a system is usually determined by injecting an unretained standard (Uracil in RP-HPLC) that has no or very little retention on a particular phase. Slight variations in this value are explained by the extra column dead volume of your specific system configuration and set-up.
Multiply the elution time of the unretained compound by the flow rate to get the actual void volume of the system and column. To determine the column void volume alone you would need to subtract the system void volume determined without the column attached.