How to scale-up a column for preparative isolation/purification from analytical conditions?
Determine optimum separation conditions by using an analytical column.
To scale-up to preparative scale, determine, set the particle size of the packing material and the inner diameter of column in consideration of the sample volume. Use SiliCycle’s scale up table (to find scale up factor) to set the proper preparative conditions.
| Length | Internal Diameter | |||||
|---|---|---|---|---|---|---|
| 4.6 mm | 10 mm | 21.2 mm | 30 mm | 50 mm | ||
| 50 mm | 0.5 - 2 | 2 - 10 | 10 - 45 | 20 - 90 | 50 - 250 | Sample Load (mg) |
| 100 mm | 1 - 5 | 4 - 25 | 20 - 105 | 40 - 210 | 120 - 600 | |
| 150 mm | 2 - 7 | 10 - 35 | 30 - 160 | 70 - 300 | 200 - 850 | |
| 250 mm | 3 - 10 | 12 - 45 | 60 - 220 | 120 - 430 | 320 - 1200 | |
| Typical Flow Rate (mL/min) | 1 - 1.5 | 4 - 10 | 20 - 30 | 40 - 70 | 110 - 250 | |
Proceed with the preparative separation
Discover our HPLC offer
Our Most Popular Categories
Our New Products
> SiliaFast FaPEx for pesticide residue analysis
> SiliaMetS E-PAK Fixed-Bed Flow Through Purification Cartridges
