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How to scale-up a column for preparative isolation/purification from analytical conditions?

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Determine optimum separation conditions by using an analytical column.

To scale-up to preparative scale, determine, set the particle size of the packing material and the inner diameter of column in consideration of the sample volume. Use SiliCycle’s scale up table (to find scale up factor) to set the proper preparative conditions.

Length Internal Diameter
4.6 mm 10 mm 21.2 mm 30 mm 50 mm
50 mm 0.5 - 2 2 - 10 10 - 45 20 - 90 50 - 250 Sample
Load
(mg)
100 mm 1 - 5 4 - 25 20 - 105 40 - 210 120 - 600
150 mm 2 - 7 10 - 35 30 - 160 70 - 300 200 - 850
250 mm 3 - 10 12 - 45 60 - 220 120 - 430 320 - 1200
Typical Flow Rate (mL/min) 1 - 1.5 4 - 10 20 - 30 40 - 70 110 - 250

 

Proceed with the preparative separation