- > Offre d'emploi: Designer Graphique
- > It’s not just about the shape – Handy guide to (other) common problems in HPLC
- > How to get the perfect shape according to experts – Top 6 most common shape problems in HPLC
- > Some People Excel at Scaling Up Their Purifications and Some Don’t, Which One Are You?
- > Loading in Progress – Avoiding loading capacity mistakes
What is the shipping solvent for your HPLC columns?
The shipping solvent, unless stated differently on the Certificate of Analysis (CoA) and/or the column box, is the solvent used on your test chromatogram to evaluate the column efficiency and peak symmetry.
Should there be a buffer in the mobile phase used to evaluate the column performance, it is always removed from the column by running the column is the same mobile phase without the buffer prior to shipping the column to the customer. Most normal phase columns (ie. Cyano (CN) and Amine (NH2)) are typically shipped in 100% Methanol (unless requested otherwise) as they are usually used in RP mode by most end-users even though they are tested in NP with hexane as the mobile phase. Unmodified Silica columns are shipped in the test mobile phase: 100% Hexane.