Elemental Analysis of Organic Compounds
SiliaFlash silica gel has a very low organic content. All lots are subjected to elemental analysis to determine the carbon, nitrogen and sulfur levels.
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SiliaPrepX - Polymeric SPE
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Purification
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Metal Removal
- 1.Typical Experimental Conditions & Calculation
- 2. Parameters Effect On Scavenging Efficiency
- 3. SiliaBond® Metal Scavengers Characteristics
- 4. Scavenging Palladium Catalysts
- 5. Scavenging Pd(Dppf)Cl2 Catalyst
- 6. Scavenging Rhodium Catalysts
- 7. Scavenging Ruthenium Catalysts
- 8. Scavenging A Nickel Catalyst
- 9. Scavenging A Platinum Catalyst
- 10. Scavenging A Tin Catalyst
- 11. SiliaBond® Metal Scavenger: Compatible With RCM Reaction
- 12. SiliaBond® (Bulk) & SiliaPrep™ (SPE) Scavenging Efficiency Comparison
- 13. SiliaBond® Metal Scavenger: Easily Scalable
- 14.Metal Scavenging Using SiliaBond® Thiol In Pictures
- 15. Metal Scavenging Using SiliaPrep™ Thiol In Pictures
- 16. Use Of SiliaBond® Cysteine
- 17. Use Of SiliaBond® DMT
- 18. Use Of SiliaBond® Imidazole
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Analysis
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Solid Phase Extraction
- Steroids Extraction By Reversed-Phase Chromatography
- SPE Extraction Of Oil & Grease From Water Using SiliaPrep™ C18 (17%)
- SPE Extraction Of Phenolic Compounds From Wine Using SiliaPrep™ C18 (17%)
- SPE Extraction Of Streptomycin From Food Using SiliaPrep™ SCX And C18 (17%)
- SPE Method For Drugs Of Abuse In Urine Sample Using SiliaPrep™ CleanDRUG
- SPE Method For Pesticides In Drinking Water Using SiliaPrep™ CleanENVI
- SPE Method For Sibutramine Detection In Human Plasma
- SPE Method For Ropinirole & Amitriptyline Dectection In Human Plasma Using SiliaPrep™ CleanDRUG
- SPE Method for Caffeine, Cotinine and Nicotine Quantification in Urine Using SiliaPrepX™ HLB
- SPE Method for Sulfonamides, Tetracyclines and Pyrimethamines Determination in Milk Using SiliaPrepX™ HLB and DVB
- SPE Method for Amphetamines Quantification in Urine Using SiliaPrepX™ HLB
- Determination of Pesticides in Drinking Water Using SiliaPrepX™ HLB
- Determination of Carbendazim in Orange Juice Using SiliaPrepX™ SCX
- SPE Method for Marbofloxacin and Sarafloxacin Determination in Salmon Using SiliaPrepX™ SCX
- SPE Method for Acrylamide Determination in Fried Potato Chips Using SiliaPrepX™ HLB and SCX
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HPLC Columns
- HPLC Column Cleaning and Regeneration
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- SiliaChrom® dt C18 - Assay For QC Testing Of Blood Pressure Medication
- SiliaChrom® SB C18 - Melamine In Food
- SiliaChrom® HILIC - Separation Of Vitamin B Complex & Vitamin C
- SiliaChrom® Amylose T-DPC - Enantiomeric Separation Of L & D-Val-PMB
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FAQ
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>> Metal & Organic Scavengers
- What are metal scavengers?
- How to calculate how much metal scavenger to use?
- How to choose a metal scavenger?
- Why Choose Silica-Based vs Polymer-Based Scavengers?
- How to purify your API from organic contaminants?
- What is a supported scavenger?
- What quantity of scavenger should be used?
- At what temperature should the scavenger be used?
- What solvents should be used with our scavengers?
- At what pH should the scavenging in aqueous solution be performed?
- Reaction time vs scavenger quantity
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>> HPLC
- What is HPLC?
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- What to do when backpressure increases?
- How do I determine the void volume in HPLC?
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- Ideal injection volume vs column dimension
- What happens if my sample solvent is stronger than my mobile phase?
- Can I get a sharper peak by injecting my sample in a weaker injection solvent?
- How much sample can I inject on my LC column?
- What size threads are on the end fittings of my HPLC column?
- Why do most users privilege PEEK vs stainless steel fittings and tubing?
- What is the internal diameter of my LC tubing?
- Can I operate my column with a 100% aqueous mobile phase?
- How can the column performance be checked?
- How can the columns be cleaned or regenerated?
- What is the appropriate flow rate for the different column dimensions?
- What is the difference between dead volume and dwell volume?
- What is the shipping solvent for your HPLC columns?
- When should I use a gradient?
- What are the common buffers used in HPLC?
- Why is my chromatogram showing peak tailing, ghost peaks, fronting peaks, split peaks / shoulder peaks or rounded peaks?
- Why is it difficult to get reproducible retention times in normal phase chromatography?
- What is the difference between a monomeric and polymeric C18 bonded phase?
- How should the columns be stored?
- How to scale-up a column for preparative isolation/purification from analytical conditions?
- Should I run a gradient from 5-95% organic solvent or from 0-100%?
- How are column efficiency, peak asymmetry factor, tailing factor and resolution calculated?
- What to do when there are no peaks, small peaks or negative peaks?
- What to do when there is no flow?
- What is the cause of variable retention times, loss of resolution or selecticity change?
- What is the cause of baseline drift or noise?
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>> Functionalized Silica
- What is the difference between silica and polymer?
- How should functionalized silica be handled?
- What are the storage conditions for the functionalized silica?
- Can functionalized silica be packed?
- Won’t the supported reagents leach since they are adsorbed and not bound?
- What is a supported reagent?
- Are all functionalized products endcapped?
- Does functionalized silica dissolve in methanol or any other standard organic solvents?
- Can different functionalized silicas be mixed?
- >> Solid Phase Extraction (SPE)
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>> Thin Layer Chromatography
- How to switch to SiliCycle TLC Plates
- What type of TLC plate to choose?
- What type of TLC backing to choose?
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- How to read a chromatogram?
- Stains for TLC Plates
- Streaking or elongated spot rather than a defined spot?
- Unable to see any spots on the TLC?
- How to monitor a reaction in presence of similar Rfs for both starting materials and product of interest?
- What is the co-spotting method?
- What to do if compounds stay too close to the baseline or solvent front?
- What is Solid Phase Extraction (SPE) ?
- What is FaPEX?
- Monomeric vs. polymeric C18
- What is Endcapping?
- What is Flow Chemistry?
- What is Microwave Chemistry?
- What is the "Catch and Release" technique?
- Why is the level of fines important?
- Are custom formats available?
- Silica Selection Guide
- >> Silica Analysis Descriptions
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>> Metal & Organic Scavengers
- Contact Us
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