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Why am I unable to visualize my spots on my TLC? Neither using UV light nor using stains works.

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Possible causes and solutions:

  • If you have not been able to visualize any spots on your TLC using UV light, try another method; maybe your compound is not UV-active.
  • Your compound might be too diluted. To solve this problem, you could try to concentrate your sample (if possible) or you can try to deposit the sample multiple times at the same place before elution (be careful to let the sample fully dry between each application to avoid creating very large spots).
  • Alternatively, your compound might also be volatile and may have evaporated from the plate. In this case, the compound will unfortunately be difficult to visualize by TLC.

 

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