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What type of TLC plate to choose?

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Various types of plates are available for thin-layer chromatography applications:

The plate types are selected based on the type of analysis required and the available budget.

Properties Classical TLC HPTLC Preparative PLC
ApplicationsQuick, inexpensive, flexible and classical separationsHighly sophisticated separation, complex samplesPurification on a TLC plate
AnalysisQualitativeQualitative & QuantitativeQuantitative
DetectionUV - StainsInstrumented analysis (use of scanners for detection)UV
PriceLower prices than HPTLCHigher prices than TLC-
Distribution
[Mean Particle Size]
5 - 20 μm [10 - 14 μm]4 - 8 μm [5 - 6 μm]5 - 40 μm [22 - 25 μm]
Layer Thickness200 - 250 μm150 - 200 μm500 - 2,000 μm
Typical Sample Volume1 - 5 μL0.1 - 0.5 μL5 - 20 μL

 

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