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SiliaBond Reversed Phases

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In reversed-phase chromatography, the packing material is always hydrophobic (non polar) while the mobile phase is polar. The more hydrophobic the packing material, the more retention of non polar analytes.

Usual reversed-phases are standard alkyl chains grafted on silica (C18, C8, C4, C1) and cyclic or aromatic functions (Phenyl, Pentafluorophenyl, Cyclohexyl).

Important parameters to keep in mind in reversed-phase chromatography:

  • Carbon load (% C) will give the relative hydrophobicity of the packing media. Most of the time, it varies between 4 % and 19 %.
  • Endcapping: it is impossible to react with all available silanol groups (free -OH groups on the silica surface) when functionalizing silica. But these free silanols are acidic and will react with basic compounds, so we endcap them with a capping agent to avoid non-specific bindings.

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  1. SiliaBond C18 (17%C) Monomeric, 40 - 63 µm, 60 Å (R33230B)

    SiliaBond C18 (17%C) Monomeric, 40 - 63 µm, 60 Å (R33230B)

    The SiliaBond® C18 16% BDS is the most efficient deactivated C18 reversed-phase. This versatile support is the best choice for your applications in reversed-phase chromatography. This chromatographic phase has been compared with different competitors and presents a better separation for basic compounds.
  2. SiliaBond C18 (13%C) WPD, 37 - 55 µm, 125 Å (R33229G)
  3. SiliaBond C8 (11%C) Monomeric (R30830B) 2
  4. SiliaBond C4 (8%C) Polymeric (R32030B)

    SiliaBond C4 (8%C) Polymeric (R32030B)

    SiliaBond C4 (or C4) provides less retention towards non-polar compounds than C18 and C8, and is useful in ion-pairing chromatography or to separate large biomolecules.  It works best for molecules with a large hydrophilic region or in case where the hydrophobic region is buried within the structure.
  5. SiliaBond C1 (5%C) Polymeric, 40 - 63 µm, 60 Å (R33030B)

    SiliaBond C1 (5%C), 40 - 63 µm, 60 Å (R33030B)

    SiliaBond® C1 (or C1) has the lowest degree of hydrophobicity of all reversed stationary phases, useful in the separation of large biomolecules that have extensive hydrophobic regions.
  6. SiliaBond Phenyl (PHE) (R34030B)

    SiliaBond Phenyl (PHE) (R34030B)

    SiliaBond Phenyl (Si-PHE) is a sorbent of medium polarity used in reversed-phase chromatography. It has similar retention than C8 but with different selectivity especially for molecules containing aromatics and fatty acids.

  7. SiliaBond Pentafluorophenyl (PFP) (R67530B)

    SiliaBond Pentafluorophenyl (PFP) (R67530B)

    SiliaBond® Pentafluorophenyl (Si-PFP) is a sorbent primarily used in the separation of molecules bearing fluorine atoms but may also be used in the separation of non-fluorous compounds such as Taxol and its derivatives. Because of its phenyl ring, Si-PFP has a higher selectivity for aromatics containing molecules compared to the other fluorinated sorbents.
  8. SiliaBond Cyano (CN, 7%C) (R38030B)

    SiliaBond Cyano (CN, 7%C) (R38030B)

    SiliaBond Cyano (Si-CN) can be used both in normal and reversed-phase chromatography as its polarity marks the separation between the polar and non-polar phases. SiliaBond Cyano is the least retentive polar sorbent in normal phase chromatography and the least retentive of the non-polar sorbents in reversed-phase chromatography.

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