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SiliCycle High Pressure Chromatography Products

With SiliaChrom HPLC columns and SiliaSphere spherical silica gels, SiliCycle offers the most reliable portfolio for high pressure chromatography, from laboratory to pilot-plant production scales.

SiliaSphere Spherical Silica Gels

For over 20 years, SiliCycle has been dedicated to silica gel manufacturing and has achieved a strong know-how and expertise in this field. Furthermore, to support the increasing demand on our spherical silicas, we have developed an optimized and highly controlled large-scale production process for all of our SiliaSphere products without decreasing the quality.

Particle shape, pore and particle size distributions, silica gel purity, and surface properties, all have their influence on chromatographic performance. Therefore, in order to develop the most efficient process, all previously mentioned parameters need to be evaluated and optimized to ensure batch-to-batch reproducibility.

SiliaSphere is manufactured from an organic form of silicon (alkoxydes). This ensures very low metal content as the starting material is purified by distillation. Deionized water is used to hydrolyze the silicon alkoxydes. Careful monitoring and control of the parameters that induce precipitation afford spherical silica gels with the desired characteristics. SiliaSphere products are characterized by a very low metal content and exceptional stability even at extreme pHs. Furthermore, our manufacturing process ensures quality and reproducibility in pore size, surface area and particle sizes and morphology for all SiliaSphere products.

SiliaSphere, the right choice for superior…

  • Chromatographic performance
  • Loading capacity
  • Reproducibility
  • Chemical & physical stability

Perfectly Spherical Particle Shape

The perfectly spherical shape of SiliaSphere silicas, combined to their smooth surfaces free of any cracks, cavities and fines make them the packing of choice for chromatography. The SiliaSphere sphericity exceeds or compares favorably to well-known brands in spherical silica gel as demonstrated by scanning electron microscope (SEM).

Narrow Pore Size Distribution

The right pore diameter to use is related to the type of molecules present in the sample to be purified. Typically, for small molecules, a 100 - 120 Å pore size is recommended. However, if higher resolution is required, then 60 - 80 Å will be more suitable. For large molecules, such as peptides and proteins, 300 Å or higher is recommended.

> read more about SiliaSphere spherical silica gels


SiliaChrom HPLC Columns

SiliCycle has been manufacturing and packing HPLC columns for more than 15 years now and we offer more than 40 different phases for your different needs. We are constantly working on enhancing that portfolio to suit your very needs and offer unique and performant new phases through our R&D facilities. Whether you require a silica-based material for use in a wide range of pH, 100% aqueous or organic mobile phases, low bleed for LC-MS applications: we have the solution for you.

Our raw materials and finished HPLC columns go through our ISO 9001-2008 registered manufacturing facilities under strict SOP’s and standard QC laboratory column performance testing assuring column performance, peak symmetry and lot-to-lot reproducibility. An incredibly wide range of selectivities, column dimensions and granulometries are available to accommodate the vast majority of your applications.

> read more about SiliaChrom HPLC columns

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