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Isolation of 13-Methyl-3,4-seco-ent-podocarpanes, rare C18-diterpenoids from the roots of Flueggea virosa by reversed-phase chromatography on SiliaBond C18

Flueggea virosa

Reversed-phase chromatography is a powerful tool in the isolation of new natural products from natural source.

Nature is a never ending source of new potential treatments for challenging diseases. In the course of a research program looking for anti-HCV agents from a medicinal plant, Flueggea virosa, fourteen new C18-diterpenoids (including a new naphtalene derivative) were isolated thanks to reversed-phase chromatography on SiliaBond C18 silica gel. Their structures were resolved by spectroscopic methods and their biological activities were tested against hepatitis C virus (HCV), with a number of structure showing promising results.

Keywords:
natural products, terpenoids, hepatitis C, C18, naphtalene derivative

> SiliaBond - Chromatographic Phases

> SiliaBond C18

 

Publication 13-Methyl-3,4-seco-ent-podocarpanes, rare C18-diterpenoids from the roots of Flueggea virosa
Authors Chih-Hua Chao*, Ju-Chien Cheng, De-Yang Shen, Hui-Chi Huang, Yang-Chang Wua and Tian-Shung Wu
Organization School of Pharmacy, China Medical University, Taichung 40402, Taiwan
Source RSC Adv., 2016, 6, 34708
Date 2016
Reaction scheme Isolation of 13-Methyl-3,4-seco-ent-podocarpanes, rare C18-diterpenoids from the roots of Flueggea virosa by reversed-phase chromatography on SiliaBond C18
Full publication http://pubs.rsc.org/is/content/articlelanding/2016/ra/c6ra00843g#!divAbstract

 

SiliaBond Chromatographic Phases

SiliCycle SiliaBond Functionalized Silica Gels for Low Pressure Chromatography

Silica is the most widely used media in chromatography. These bare and grafted supports possess great properties for use as stationary phases and are particularly appreciated for their high mechanical resistance.

SiliaBond Reversed-phase

In reversed-phase chromatography, the packing material is always non-polar (hydrophobic) while the mobile phase is polar to non-polar. An important parameter affecting chromatographic efficiency is the hydrophobicity of the sorbent. As a general rule, stationary phase hydrophobicity increases with the alkyl chain length.

SiliCycle developed an innovative C18 chromatographic phase (PN: R33230B) characterized by a homogeneous coverage of the alkyl chains on the surface. Consequently, the endcapping step is more controlled, which leads to much improved separations and inhibition of the non-specific interactions with silanol groups (highly deactivated silanol phase). Compared to competitive products, this endcapped C18 phase with 17 % carbon load exhibits high hydrophobicity and base deactivated properties.

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