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SiliaChrom®- XDB HPLC Columns

SiliaChrom AQ C18 columns use a new generation adsorbent based on high purity spherical silica and unique bonding technology. SiliaChrom AQ C18 is a slightly polar C18 column, and is the most versatile reversed phase column offered by SiliCycle for highly aqueous mobile phases. SiliaChrom AQ C18 is considered as the most universal HPLC column for any type of analytes (acidic, basic and neutral). Furthermore, SiliaChrom AQ C18 is applicable to a wide range of sample types: plasma, urine, drug formulation, food extraction, forensic, clinical and bioanalytical.



Main Characteristics

  • Exceptional stability at pH 1,5 to 9,0
  • Inertness for acidic and basic analytes
  • Compatible from 100% aqueous mobile phase to 100% organic
  • Rapid equilibration
  • Reduced need for mobile phase modifiers
  • Partially endcapped

More Information


Columns Pore Size Particle Size Surface Area Carbon Loading
C18 100Å 3, 5 µm 380 m2/g 18%
Columns pH Range Temperature Limit* Pressure Limit USP Code
C18 1,5-9,0 65°C 5000 psi L01

* At pH range 5 to 7,5

Available column types: Fast analysis, Analytical, Semi-Preparative, Preparative 


Application Notes

SiliaChrom AQ C18 - Cefaclor an Antibiotic Soluble in Water

SiliaChrom AQ C18 - Universal HPLC Column

Evaluation of Resolution and Peak Shape of Acidic, Basic and Neutral Commercial Drugs

SiliaChrom AQ C18, 5 µm, 4,6x150 mm (SiliCycle P/N: HPC-1405-15N)
MPA: 5mM potassium monobasic (adjust to pH 2,5 with H3PO4)/ACN (90/10)
MPB: 5mM potassium monobasic (adjust to pH 2,5 with H3PO4)/ACN (10/90)
Gradient: MPA to MPB, 20 minutes
Temperature: 23°C
Flow rate: 1,0 mL/min
Detector: UV at 230 nm
Injection volume: 20 µL


Drugs Retention Time Peak Asymmetry Factor Theoretical Plates

(min) (USP) (USP)
Maleic acid 2,46 1,84 7876
Acetaminophen 5,53 1,04 32409
Chlorpheniramine 7,17 1,32 63543
Benzoic acid 10,75 1,03 93431
Salicylic acid 11,81 1,04 102119
Dichlorophen 17,83 1,01 231238
Ibuprofen 18,22 1,00 209557


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