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SiliaChrom® - dt Reversed Phase HPLC Columns

SiliaChrom dt C18 has the same unique technology phase as SiliaChrom AQ C18, but the purity of the spherical silica gel is 99,999%, the highest value for HPLC SiliaChrom Series. This phase has no metal content, in order to avoid secondary interaction with basic or ion species. SiliaChrom dt C18 is made with a true universal reversed-phase sorbent that can be used for separation of acidic, basic and neutral molecules. It is suitable for analysis of stringent QA/QC testing because it has a superior performance than any C18 phases in almost every aspects. SiliaChrom dt C18 is an interesting environmental and pharmaceutical phases.



Main Characteristics

  • Enhances retention of hydrophilic molecules
  • Low bleed and high sensitivity for LC-MS
  • Extremely low metal content (< 10 ppm)
  • Good tolerance for directly injected biological matrix (dirty samples)
  • Higher surface area
  • Partially endcapped

More Information


Columns Pore Size Particle Size Surface Area Carbon Loading
C18 100 Å 2.5, 3, 5 µm 410-441 m2/g 18%
Columns pH Range Temperature Limit* Pressure Limit USP Code
C18 1,5-9,0 65° C 5000 psi L01

* At pH range 5 to 7,5

Available column types: Fast analysis, Analytical, Semi-Preparative, Preparative 

Application Note

SiliaChrom dt C18 - Assay for QC Testing of Blood Pressure and Cholesterol Medication

Evaluation of Resolution and Peak Shape of Chlorinated Herbicides

SiliaChrom dt C18, 5 µm, 4,6x150 mm (SiliCycle P/N: HPC-14303-15N)
5 mM potassium phosphate monobasic (adjust to pH 2,5 with H3PO4)/ACN
Temperature: 23°C
Flow rate: 1,0 mL/min
Detector: UV at 210 nm
Injection volume: 10 µL


Chlorinated Herbicides Retention Time Peak Asymmetry Factor Theoretical Plates

(min) (USP) (USP)
Dicamba 7,05 1,01 12746
Atrazine 9,77 1,00 12546
2,4-Dichlorophenoxyacetic acid 10,63 1,02 12736

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