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SiliaChrom®- XDB2 HPLC Columns

SiliaChrom XDB2 C18 is designed to present a mid-hydrophobic C18 phase with 18% of carbon loading like most of the popular reversed-phase HPLC columns on the market. This phase presents a balanced hydrophobic adsorption in order to avoid excessive retention of hydrophobic compounds.



Main Characteristics

  • Great column-to-column and batch-to-batch reproducibility (popular for QC/QA laboratory)
  • Typical average value for carbon loading (18%)
  • Good peak shape for basic, acidic and neutral analytes
  • Stronger separation power for isomers
  • Double endcapped

More Information


Columns Pore Size Particle Size Surface Area Carbon Loading
C18 100Å 3, 5 µm 380 m2/g 18%
Columns pH Range Temperature Limit* Pressure Limit USP Code
C18 1,5-9,0 65°C 5000 psi L01

* At pH range 5 to 7,5

Available column types: Fast analysis, Analytical, Semi-Preparative, Preparative


Application Note

Evaluation of SiliaChrom XDB2 C18 by USP and NIST Approches
(Pharmacopeia Forum 31 (2005) 637, NIST SRM 870 certificate issue: 19th december 2002 valid until: 30th september 2010)

SiliaChrom XDB2 C18, 5 µm, 4,6x150 mm
Methanol/10 mM potassium phosphate monobasic + 10 mM potassium phosphate dibasic (pH: 7,00) (80/20)
Temperature: 23,0°C
Flow Rate: 1,00 mL/min
Detector: UV 254 nm
Injection Volume: 1 µL


Compounds Retention Time Peak Asymmetry Factor Theoretical Plates

(min) (USP) (USP)
Uracil 1,61 1,24 4618
Toluene 4,73 1,04 12858
Ethylbenzene 6,19 1,00 13633
Quinirazine 11,18 1,03 12277
Amitriptyline 13,53 1,29 9451


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