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SiliaChrom HPLC Column Selection Guide by Manufacturer
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To find a suitable SiliCycle alternative to another manufacturer's columns, refer to the selection guide below.
SiliCycle's alternative phases are selected based on physical and chemical similarities. These alternatives are not guaranteed to provide the same retention or selectivity, but should be sufficiently similar in character to allow a similar or improved separation to be achieved after some method optimization.
The user should refer to the individual phase information to ensure that the characteristics of the alternative match the requirements of their separation. SiliCycle takes no responsibility for any error or omission relating to this information. All trademarks and registered trademarks are the property of their respective owners.
Manufacturers
> Advanced Chromatography Technologies™ (ACT)
> Agilent Technologies™
> GL Sciences™
> Grace Discovery Sciences™
> Kromasil™
> Agilent Technologies™
> GL Sciences™
> Grace Discovery Sciences™
> Kromasil™
Advanced Chromatography Technologies™ (ACT)
| Phase | Pore size | Surface Area | Carbon Load | Endcapping | Recommended Alternative |
|---|---|---|---|---|---|
| ACE™ C18 100 | 100 Å | 300 m2/g | 15.5 % | NF | SiliaChrom Plus C18 |
| ACE™ C8 | 100 Å | 300 m2/g | 9 % | NF | SiliaChrom Plus C8 |
| ACE™ Phenyl | 100 Å | 300 m2/g | 9.5 % | NF | SiliaChrom Plus Phenyl |
| Ace™ AQ | 100 Å | 300 m2/g | 14 % | NF | SiliaChrom dt C18 |
| Ace™ C18-300 | 300 Å | 100 m2/g | 9 % | Yes | SiliaChrom Plus C18-300 |
| Ace™ C8-300 | 300 Å | 100 m2/g | 5 % | Yes | SiliaChrom Plus C8-300 |
| Ace™ C4-300 | 300 Å | 100 m2/g | 2.6 % | Yes | SiliaChrom Plus C4-300 |
Agilent Technologies™
| Phase | Pore size | Surface Area | Carbon Load | Endcapping | Recommended Alternative |
|---|---|---|---|---|---|
| Zorbax™ SB-C18 | 300 Å | 45 m2/g | 3 % | No | SiliaChrom Plus C18-300 |
| Zorbax™ SB-C8 | 300 Å | 45 m2/g | 2 % | No | SiliaChrom Plus C8-300 |
GL Sciences™
| Phase | Pore size | Surface Area | Carbon Load | Endcapping | Recommended Alternative |
|---|---|---|---|---|---|
| Inertsil™ C4 | 150 Å | 320 m2/g | 8 % | Yes | SiliaChrom Plus C4 |
| Inertsil™ Phenyl | 150 Å | 320 m2/g | 10 % | Yes | SiliaChrom Plus Phenyl |
| Inertsil™ C8 | 150 Å | 320 m2/g | 11 % | Yes | SiliaChrom Plus C8 |
| Inertsil™ ODS2 | 150 Å | 320 m2/g | 18.5 % | Yes | SiliaChrom dt C18 |
| Inertsil™ ODS3 or ODS3V | 100 Å | 450 m2/g | 15 % | Yes | SiliaChrom Plus C18 |
Grace Discovery Sciences™
| Phase | Pore size | Surface Area | Carbon Load | Endcapping | Recommended Alternative |
|---|---|---|---|---|---|
| Alltima™ HP C18 AQ | 100 Å | 450 m2/g | 2 % | Yes | SiliaChrom dt C18 |
| Vydac™ 208TP | 300 Å | 60-110 m2/g | 8 % | Yes | SiliaChrom Plus C8-300 |
| Vydac™ 214TP | 300 Å | 60-110 m2/g | 4 % | Yes | SiliaChrom Plus C4-300 |
| Vydac™ 218TP | 300 Å | 60-110 m2/g | 8 % | Yes | SiliaChrom Plus C18-300 |
Kromasil™
| Phase | Pore size | Surface Area | Carbon Load | Endcapping | Recommended Alternative |
|---|---|---|---|---|---|
| Kromasil™ C18 | 100 Å | 340 m2/g | 19 % | Yes | SiliaChrom dt C18 |
| Kromasil™ C8 | 100 Å | 340 m2/g | 12 % | Yes | SiliaChrom Plus C8 |
| Kromasil™ C4 | 100 Å | 340 m2/g | 8 % | Yes | SiliaChrom Plus C4 |
| Kromasil™ Silica | 100 Å | 340 m2/g | - | No | SiliaChrom Plus Silica |
Macherey-Nagel™
| Phase | Pore size | Surface Area | Carbon Load | Endcapping | Recommended Alternative |
|---|---|---|---|---|---|
| Nucleodur™ C18ec | 110 Å | 340 m2/g | 18 % | Yes | SiliaChrom dt C18 |
| Nucleodur™ C18 | 110 Å | 340 m2/g | 18 % | Yes | SiliaChrom dt C18 |
| Nucleodur™ CN | 110 Å | 340 m2/g | 7 % | Yes | SiliaChrom Plus CN |
| Nucleosil™ C18 | 100 Å | 350 m2/g | 15 % | Yes | SiliaChrom Plus C18 |
| Nucleosil™ C8 | 100 Å | 350 m2/g | 9 % | No | SiliaChrom Plus C8 |
| Nucleosil™ C6H5 (Phenyl) | 100 Å | 350 m2/g | 8 % | No | SiliaChrom Plus Phenyl |
| Nucleosil™ CN | 100 Å | 350 m2/g | 5 % | No | SiliaChrom Plus CN |
| Nucleosil™ NH2 | 100 Å | 350 m2/g | 3.5 % | No | SiliaChrom Plus NH2 |
| Nucleosil™ OH (Diol) | 100 Å | 350 m2/g | NF | NF | SiliaChrom Plus Diol |
| Nucleosil™ SA | 100 Å | 350 m2/g | 7 % | No | SiliaChrom Plus SCX |
| Nucleosil™ Silica | 100 Å | 350 m2/g | - | No | SiliaChrom Plus Silica |
| Nucleosil™ 300 C18 | 300 Å | 100 m2/g | 6.5 % | Yes | SiliaChrom Plus C18-300 |
| Nucleosil™ 300 C8 | 300 Å | 100 m2/g | 3 % | Yes | SiliaChrom Plus C8-300 |
| Nucleosil™ 300 C4 | 300 Å | 100 m2/g | 2 % | Yes | SiliaChrom Plus C4-300 |
Phenomenex™
| Phase | Pore size | Surface Area | Carbon Load | Endcapping | Recommended Alternative |
|---|---|---|---|---|---|
| AQUA™ C18 | 125 Å | 320 m2/g | 15 & | Yes, proprietary | SiliaChrom dt C18 |
| Luna™ C18 (2) | 100 Å | 400 m2/g | 17.5 % | Yes | SiliaChrom Plus C18 |
| Luna™ C8 | 100 Å | 440 m2/g | 14.75 % | Yes | SiliaChrom Plus C8 |
| Luna™ C8 (2) | 100 Å | 400 m2/g | 13.5 % | Yes | SiliaChrom Plus C8 |
| Luna™ CN | 100 Å | 400 m2/g | 7 & | Yes | SiliaChrom Plus CN |
| Luna™ NH2 | 100 Å | 400 m2/g | 9.5 % | No | SiliaChrom Plus NH2 |
| Luna™ PFP (2) | 100 Å | 400 m2/g | 11.5 % | Yes | SiliaChrom Plus PFP |
| Luna™ SCX | 100 Å | 400 m2/g | N/A | No | SiliaChrom Plus SCX |
| Luna™ Silica (2) | 100 Å | 400 m2/g | - | No | SiliaChrom Plus Silica |
| Synergi™ Hydro-RP | 80 Å | 475 m2/g | 19 % | Yes, proprietary | SiliaChrom dt C18 |
| Prodigy™ C8 | 150 Å | 310 m2/g | 13 % | Yes | SiliaChrom Plus C8 |
| Prodigy™ ODS2 | 150 Å | 310 m2/g | 18 % | Yes | SiliaChrom Plus C18 |
| Prodigy™ ODS-3 | 100 Å | 450 m2/g | 16 % | Yes | SiliaChrom Plus C18 |
| Prodigy™ ODS-3V | 100 Å | 450 m2/g | 16 % | NF | SiliaChrom Plus C18 |
| Gemini™ C18 | 110 Å | 375 m2/g | 14 % | Yes | SiliaChrom Plus C18 |
| Jupiter™ C18 | 300 Å | 170 m2/g | 13 % | Yes | SiliaChrom Plus C18-300 |
| Jupiter™ C4 | 300 Å | 170 m2/g | 5 % | Yes | SiliaChrom Plus C4-300 |
Supelco™
| Phase | Pore size | Surface Area | Carbon Load | Endcapping | Recommended Alternative |
|---|---|---|---|---|---|
| Ascentis™ C18 | 100 Å | 450 m2/g | 25 % | Yes | SiliaChrom Plus C18 |
| Ascentis™ C8 | 100 Å | 450 m2/g | 15 % | Yes | SiliaChrom Plus C8 |
Thermo Scientific™
| Phase | Pore size | Surface Area | Carbon Load | Endcapping | Recommended Alternative |
|---|---|---|---|---|---|
| Syncronis™ C18 | 100 Å | 320 m2/g | 16 % | Yes | SiliaChrom Plus C18 |
| Syncronis™ C8 | 100 Å | 320 m2/g | 10 % | Yes | SiliaChrom Plus C8 |
| Syncronis™ AQ | 100 Å | 320 m2/g | 19 % | Yes, polar | SiliaChrom dt C18 |
| Syncronis™ Phenyl | 100 Å | 320 m2/g | 11 % | Yes | SiliaChrom Plus Phenyl |
| Syncronis™ Amino | 100 Å | 320 m2/g | 4 % | Yes | SiliaChrom Plus Amino |
| Syncronis™ Silica | 100 Å | 320 m2/g | - | No | SiliaChrom Plus Silica |
| Acclaim™ 120 C18 | 120 Å | 300 m2/g | 18 % | Yes | SiliaChrom Plus C18 |
| Acclaim™ 300 C18 | 300 Å | 100 m2/g | 8 % | Yes | SiliaChrom Plus C18-300 |
Waters Corporation™
| Phase | Pore size | Surface Area | Carbon Load | Endcapping | Recommended Alternative |
|---|---|---|---|---|---|
| Atlantis™ T3 | 100 Å | 300 m2/g | 14 % | Yes | SiliaChrom dt C18 |
| SunFire™ C18 | 100 Å | 340 m2/g | 16 % | Yes | SiliaChrom Plus C18 |
| SunFire™ C8 | 100 Å | 340 m2/g | 12 % | Yes | SiliaChrom Plus C8 |
| SunFire™ Silica | 100 Å | 340 m2/g | NF | NF | SiliaChrom Plus Silica |
| Symmetry™ C18 | 100 Å | 335 m2/g | 19 % | Yes | SiliaChrom dt C18 |
| Symmetry™ C8 | 100 Å | 335 m2/g | 12 % | Yes | SiliaChrom Plus C8 |
YMC™
| Phase | Pore size | Surface Area | Carbon Load | Endcapping | Recommended Alternative |
|---|---|---|---|---|---|
| YMC-Pack™ C4 | 120 Å | 300 m2/g | 7 % | Yes | SiliaChrom Plus C4 |
| YMC-Pack™ C8 | 120 Å | 300 m2/g | 10 % | Yes | SiliaChrom Plus C8 |
| YMC-Pack™ ODS-AQ | 120 Å | 300 m2/g | 14 % | Yes, proprietary | SiliaChrom dt C18 |
| YMC-Pack™ ODS-A | 120 Å | 300 m2/g | 17 % | Yes | SiliaChrom Plus C18 |
| YMC-Pack™ ODS-A | 300 Å | 150 m2/g | 7 % | Yes | SiliaChrom Plus C18-300 |
| YMC-Pack™ CN | 120 Å | 300 m2/g | 7 % | Yes | SiliaChrom Plus CN |
| YMC-Pack™ Diol-NP | 120 Å | 300 m2/g | NF | NF | SiliaChrom Plus Diol |
| YMC-Pack™ NH2 | 120 Å | 300 m2/g | NF | NF | SiliaChrom Plus NH2 |
| YMC-Pack™ Phenyl | 120 Å | 300 m2/g | 9 % | Yes | SiliaChrom Plus Phenyl |
| YMC-Pack™ Pro C18 | 120 Å | 350 m2/g | 16 % | Yes | SiliaChrom Plus C18 |
| YMC-Pack™ Si | 120 Å | 300 m2/g | - | No | SiliaChrom Plus Silica |
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