SiliaChrom HPLC Guard Cartridges - Ion Exchange Phases
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Purification
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Metal Removal
- 1.Typical Experimental Conditions & Calculation
- 2. Parameters Effect On Scavenging Efficiency
- 3. SiliaBond® Metal Scavengers Characteristics
- 4. Scavenging Palladium Catalysts
- 5. Scavenging Pd(Dppf)Cl2 Catalyst
- 6. Scavenging Rhodium Catalysts
- 7. Scavenging Ruthenium Catalysts
- 8. Scavenging A Nickel Catalyst
- 9. Scavenging A Platinum Catalyst
- 10. Scavenging A Tin Catalyst
- 11. SiliaBond® Metal Scavenger: Compatible With RCM Reaction
- 12. SiliaBond® (Bulk) & SiliaPrep™ (SPE) Scavenging Efficiency Comparison
- 13. SiliaBond® Metal Scavenger: Easily Scalable
- 14.Metal Scavenging Using SiliaBond® Thiol In Pictures
- 15. Metal Scavenging Using SiliaPrep™ Thiol In Pictures
- 16. Use Of SiliaBond® Cysteine
- 17. Use Of SiliaBond® DMT
- 18. Use Of SiliaBond® Imidazole
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Solid Phase Extraction
- Steroids Extraction By Reversed-Phase Chromatography
- SPE Extraction Of Oil & Grease From Water Using SiliaPrep™ C18 (17%)
- SPE Extraction Of Phenolic Compounds From Wine Using SiliaPrep™ C18 (17%)
- SPE Extraction Of Streptomycin From Food Using SiliaPrep™ SCX And C18 (17%)
- SPE Method For Drugs Of Abuse In Urine Sample Using SiliaPrep™ CleanDRUG
- SPE Method For Pesticides In Drinking Water Using SiliaPrep™ CleanENVI
- SPE Method For Sibutramine Detection In Human Plasma
- SPE Method For Ropinirole & Amitriptyline Dectection In Human Plasma Using SiliaPrep™ CleanDRUG
- SPE Method for Caffeine, Cotinine and Nicotine Quantification in Urine Using SiliaPrepX™ HLB
- SPE Method for Sulfonamides, Tetracyclines and Pyrimethamines Determination in Milk Using SiliaPrepX™ HLB and DVB
- SPE Method for Amphetamines Quantification in Urine Using SiliaPrepX™ HLB
- Determination of Pesticides in Drinking Water Using SiliaPrepX™ HLB
- Determination of Carbendazim in Orange Juice Using SiliaPrepX™ SCX
- SPE Method for Marbofloxacin and Sarafloxacin Determination in Salmon Using SiliaPrepX™ SCX
- SPE Method for Acrylamide Determination in Fried Potato Chips Using SiliaPrepX™ HLB and SCX
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Solid Phase Extraction
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Application Notes
- Suzuki Catalysts Scavenging Study with Activated Carbon and SiliaMetS
- Amine sorbent for the separation of polar compounds
- Using Silica-Based Products for Food and Drug Applications
- Plastic Chemical Resistance Guide
- Reversed-Phase Chromatography: General Introduction for Improved Method Development
- What is Endcapping and Why Do it?
- Experimental Procedure Optimization for SiliaMetS Metal Scavengers
- Recommended Storage Condition for Functionalized SiliaSep Cartridges
- SiliaSep Flash Cartridges System Compatibility
- Working with Silver Nitrate (AgNO3 ) Silica-based Products
- Efficient Use of E-PAK Technology for the Removal of a Widely Used Suzuki Catalyst
- Advantages of SiliaSphere PC Spherical Silica over SiliaFlash Irregular Silica
- Working with Fluorinated Silica Phases
- Working with Diol silica phase
- Working with Cyano silica phase
- Working with Amine (WAX) silica phase
- Working with Ion Exchange Silica Phases
- Efficient use of the E-PAK technology for removing iridium
- Using E-PAK technology to remove chlorophyll in a biomass sample
- Rhodium Scavenging Study with Activated Carbon and SiliaMetS
- Efficient method for the removal of nickel with E-PAK
- HPLC Columns - Cleaning & Regeneration Procedures
- Working with reversed-phases
- Comparing Silica and Polymer Supported Products
- TLC Troubleshooting with SiliCycle’s SiliaPlate
- SiliaPrep SAX-2 nec (TMA Acetate) SPE Cartridges
- The Brockmann & Schodder Activity Test
- Screening and Efficient Removal of Ruthenium 3rd Generation Grubbs Catalyst
- Scavenging Using SiliaBond Carbonate
- SPE Extraction Using SiliaPrep SCX & SCX-2
- TLC Plate Selection Guide
- Understanding Loading Capacities of SiliaSep Cartridges
- Scaling up a Sonogashira Reaction with E-PAK
- Choosing Between Spherical Silica Gel (SiliaSep PREMIUM) and Irregular Silica Gel (SiliaSep) for Flash Cartridges
- Purification of a Heterocyclic Mixture Using SiliaSep PREMIUM Cartridges
- Separation of Caffeine and Other Natural Stimulants Using Reversed-Phase C18 SiliaSep Flash Cartridges
- SiliaMetS Thiol and DMT Stability in Aqueous Media
- Comparison Between SiliaMetS Metal Scavengers and Other Sorbents
- Different Ways of Removing Rhodium
- SiliaBond Cyanoborohydride as a Reducing Agent
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Case Studies
- A Practical Cleavage of Acetals Using SiliaMetS Thiol
- Screening of Various Metal Scavengers for Copper Removal in the Synthesis of a Bicyclo Derivative
- Scavenging of the Residual Buchwald-Hartwig Catalyst with SiliaMetS Thiol
- Efficiency of SiliaMetS Triamine for Copper Removal in the Preparation of Functionalized Phosphines by CuAA
- Enantiomer N-1 Substituted Tetrazole Prodrug Synthesis
- Synthesis of Cycle-Tail Peptides in the Pseudacyclins A-E Family
- Organic Acids Quantification in Cranberry Juice
- Identification of the Chemical Composition of the American Dwarf Birch's Leaves
- Separation of Palladium and Silver from E-Waste Leachate Using SiliaMetS Thiol
- Improved Synthetic Route and Scale-Up of GSK973 using SiliaMetS Thiol for Pd Scavenging
- E-Book: SiliaMetS® Scale-Up Case Studies
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FAQ
- >> Flash Cartridges
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>> Metal & Organic Scavengers
- What are metal scavengers?
- How to calculate how much metal scavenger to use?
- How to choose a metal scavenger?
- Why Choose Silica-Based vs Polymer-Based Scavengers?
- How to purify your API from organic contaminants?
- What is a supported scavenger?
- What quantity of scavenger should be used?
- At what temperature should the scavenger be used?
- What solvents should be used with our scavengers?
- At what pH should the scavenging in aqueous solution be performed?
- Reaction time vs scavenger quantity
- What is the thermal stability of the SiliaMetS?
- What is the molecular loading and how do you determine the molecular loading of a SiliaMetS metal scavenger?
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>> HPLC
- What is HPLC?
- HPLC Columns Use Instructions
- What to do when backpressure increases?
- How do I determine the void volume in HPLC?
- Why should I use a guard column with my analytical or preparative column?
- Ideal injection volume vs column dimension
- What happens if my sample solvent is stronger than my mobile phase?
- Can I get a sharper peak by injecting my sample in a weaker injection solvent?
- How much sample can I inject on my LC column?
- What size threads are on the end fittings of my HPLC column?
- Why do most users privilege PEEK vs stainless steel fittings and tubing?
- What is the internal diameter of my LC tubing?
- Can I operate my column with a 100% aqueous mobile phase?
- How can the column performance be checked?
- How can the columns be cleaned or regenerated?
- What is the appropriate flow rate for the different column dimensions?
- What is the difference between dead volume and dwell volume?
- What is the shipping solvent for your HPLC columns?
- When should I use a gradient?
- What are the common buffers used in HPLC?
- Why is my chromatogram showing peak tailing, ghost peaks, fronting peaks, split peaks / shoulder peaks or rounded peaks?
- Why is it difficult to get reproducible retention times in normal phase chromatography?
- What is the difference between a monomeric and polymeric C18 bonded phase?
- How should the columns be stored?
- How to scale-up a column for preparative isolation/purification from analytical conditions?
- Should I run a gradient from 5-95% organic solvent or from 0-100%?
- How are column efficiency, peak asymmetry factor, tailing factor and resolution calculated?
- What to do when there are no peaks, small peaks or negative peaks?
- What to do when there is no flow?
- What is the cause of variable retention times, loss of resolution or selecticity change?
- What is the cause of baseline drift or noise?
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>> Functionalized Silica
- What is the difference between silica and polymer?
- How should functionalized silica be handled?
- What are the storage conditions for the functionalized silica?
- Can functionalized silica be packed?
- Won’t the supported reagents leach since they are adsorbed and not bound?
- What is a supported reagent?
- Are all functionalized products endcapped?
- Does functionalized silica dissolve in methanol or any other standard organic solvents?
- Can different functionalized silicas be mixed?
- >> Solid Phase Extraction (SPE)
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>> Thin Layer Chromatography
- How to switch to SiliCycle TLC Plates
- What type of TLC plate to choose?
- What type of TLC backing to choose?
- How to cut TLC plates on glass support?
- How to read a chromatogram?
- Stains for TLC Plates
- Streaking or elongated spot rather than a defined spot?
- Unable to see any spots on the TLC?
- How to monitor a reaction in presence of similar Rfs for both starting materials and product of interest?
- What is the co-spotting method?
- What to do if compounds stay too close to the baseline or solvent front?
- What is Solid Phase Extraction (SPE) ?
- Monomeric vs. polymeric C18
- What is Endcapping?
- What is Flow Chemistry?
- What is Microwave Chemistry?
- What is the "Catch and Release" technique?
- Why is the level of fines important?
- Are custom formats available?
- Silica Selection Guide
- >> Silica Analysis Descriptions
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