Silica Selection Guide
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SiliCycle offers a wide range of SiliaFlash products to cover many types of applications. Selecting the most appropriate sorbent for any given application can be difficult. To help you choose the right media (bonded or not), our experts recommend using the diagram below as a guide. Simply follow the three pathways to select the most suitable sorbent.
Selecting Pore Size
| Sample molecular weight (MW in Dalton) |
||||
| ⇙ | ⇓ | ⇘ | ||
| Organic Molecules | Peptides | Proteins (Polypeptides) Bio-polymers |
||
| ⇓ | ⇓ | ⇓ | ||
| MW < 1,000 Dalton | 1,000 Dalton < MW < 10,000 Dalton | MW > 10,000 Dalton | ||
| ⇓ | ⇓ | ⇓ | ||
| 30, 40 Â (< 400 Dalton) 60, 70 Â (400 - 1,000 Dalton) |
90, 100, 120, 150, 300 Â | 500, 800, 1,000 Â |
Selecting Particle Size
| Generated Pressure (GP) or Selectivity (α) |
||||||
| ⇙ | ⇓ | ⇓ | ⇘ | |||
| GP ≈ 950 psi (65 bar) α < 1.2 (very difficult separation) |
GP ≈ 220 psi (15 bar) 1.2 < α < 1.5 (difficult separation) |
GP ≈ 73 psi (5 bar) 1.5 < α < 2 (possible separation) |
GP ≈ 29 psi (2 bar) α > 2 (easy separation) |
|||
| ⇓ | ⇓ | ⇓ | ⇓ | |||
| From 0 to 30 µm | From 15 to 40 µm | From 40 to 100 µm | From 40 to 1,000 µm | |||
| Selectivity (α) is measured by the retention factor ratio between similar compounds: | α = | Tr1 - T0 |
| Tr2 - T0 |
A Particle Size for Each Application
This chart will help you select the right SiliaFlash irregular silica gel and particle size, depending on the targeted application.
| Particle Size Distribution | Application |
|---|---|
| Particles for Preparative TLC Plates | |
| From 0 to 20 μm |
|
| Particles for Difficult Separations | |
| From 10 to 45 μm |
|
| Particles for Flash Chromatography | |
| 40 - 63 µm |
|
| 60 - 120 µm |
|
| Particles for column (or Gravity) Chromatography | |
| From 60 - 200 µm |
|
| 120 - 200 µm |
|
| Other Application | |
| From 200 to 1,000 µm |
|
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