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SiliaSep Cartridges Characteristics

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SiliaSep Flash Cartridge Design

SiliaSep Flash Cartridge design

 

SiliaSep Features & Benefits

Speed, Reliability & Selectivity
With SiliCycle’s SiliaSep Flash Cartridges you will benefit from the same quality that all our products are known for.
We have the best silica gel available on the market with no fines.

Features Benefits
Highest silica gel quality with no fines
  • No product contamination
  • Homogeneous packing, no channelling (no peak tailing)
  • High loading capacity (high surface area)
  • Direct transfer from TLC to flash chromatography (same silica)
Innovative packing technology
  • Consistent packing for reproducible high plate count (N)
  • Superior performance & separation
  • Higher resolution with improved band definition (no tailing)
  • Greater compound purity & higher recovery
Versatility
  • Wide choice of cartridge sizes from 4 grams up to 1.5 kg
  • Purification scale-up from milligram to hundreds of grams!
  • Variety of sorbents to address any separation need
Reproducibility, reliability & safety
  • Leak-free guaranteed by unique one-piece cartridge design
  • Reproducible performance from lot-to-lot (stringent quality control)
  • Excellent durability to withstand high pressures
  • Universal luer fittings for compatibility with any flash systems
Cost effective
  • Excellent ratio performance vs price
  • Readily available from stock inventory for many volumes

SiliCycle evaluated the performance of SiliaSep cartridges against some established players in chromatography and purification. In this study, cartridge performances were evaluated by the determination of different parameters including plate count (N), reduced plate height (h), symmetry index (SI10%) and resolution factor (R). In all cases, SiliaSep allows excellent performance over the competition.

 

SiliaSep Cartridge Types Overview

Characteristics Units SiliaSep 4 g SiliaSep 12 g SiliaSep 25 g SiliaSep 40 g SiliaSep 80 g
Cartridge Code - ISO04 ISO12 ISO25 ISO40 ISO80
Silica weight g Bare: 4 g
Bonded: ≥ 5 g
Bare: 12 g
Bonded: ≥ 15 g
Bare: 25 g
Bonded: ≥ 30 g
Bare: 40 g
Bonded: ≥ 40 g
Bare: 80 g
Bonded: ≥ 90 g
#/box unit Bare: 20
Bonded: 2
Bare: 20
Bonded: 1
Bare: 15
Bonded: 1
Bare: 15
Bonded: 1
Bare: 12
Bonded: 1
Dimension
(OD x Length)
mm 16 x 98 25 x 117 25 x 165 32 x 169 36 x 237
Column volume mL 4.9 17 31 47 123
Recom. flow rate mL/ min 15 - 25 20 - 40 20 - 45 25 - 50 40 - 80
Loading capacity g 0.005 - 0.4 0.015 - 1.2 0.025 - 2.5 0.05 - 4.0 0.10 - 8.0

Characteristics Units SiliaSep 120 g SiliaSep 220 g SiliaSep 330 g SiliaSep XL 800 g SiliaSep XL 1600 g
Cartridge Code - IS120 IS220 IS330 IS750 I1500
Silica weight g Bare: 120 g
Bonded: ≥ 130 g
Bare: 220 g
Bonded: ≥ 230 g
Bare: 330 g
Bonded: ≥ 360 g
Bare: 800 g
Bonded: ≥ 870 g
Bare: 1,600 g
Bonded: ≥ 1,700 g
#/box unit Bare: 10
Bonded: 1
Bare: 4
Bonded: 1
Bare: 4
Bonded: 1
Bare: 2
Bonded: 1
Bare: 2
Bonded: 1
Dimension
(OD x Length)
mm 42 x 256 66 x 195 66 x 268 89 x 382 104 x 429
Column volume mL 190 306 441 1,500 2,900
Recom. flow rate mL/ min 60 - 120 60 - 180 80 - 180 200 - 300 300 - 450
Loading capacity g 0.15 - 12.0 0.25 - 22.0 0.50 - 33.0 0.80 - 80.0 1.60 - 160.0

How to create product number:
Product Number => FLH - [Chemistry Code] - [Cartridge Code] Ex. cartridge 4 g with silica gel => FLH-R10030B-ISO04

 

SiliaSep Loading Chart

Dimensions
OD x L (mm)
SiliaSep
Format
ΔCV =
0.1-0.6 Load (g)
ΔCV =
0.7-1.2 Load (g)
ΔCV =
1.3-1.8 Load (g)
ΔCV =
1.9-2.4 Load (g)
ΔCV =
2.5-3.1 Load (g)
16 x 98 4 g 0.040 0.080 0.120 0.160 0.200
25 x 117 12 g 0.120 0.240 0.360 0.480 0.600
25 x 165 25 g 0.250 0.500 0.750 1.000 1.250
32 x 169 40 g 0.400 0.800 1.200 1.600 2.000
36 x 237 80 g 0.800 1.600 2.400 3.200 4.000
42 x 256 120 g 1.200 2.400 3.600 4.800 6.000
66 x 195 220 g 2.200 4.400 6.600 8.800 11.000
66 x 268 330 g 3.300 6.600 9.900 13.200 16.500
89 x 382 800 g 8.000 16.000 24.000 32.000 40.000
120 x 429 1 600 g 10.000 20.000 30.000 40.000 50.000

Dimensions
OD x L (mm)
SiliaSep
Format
ΔCV =
3.2-3.8 Load (g)
ΔCV =
3.9-4.5 Load (g)
ΔCV =
4.6-5.2 Load (g)
ΔCV =
5.3-6.0 Load (g)
ΔCV =
> 6 Load (g)
16 x 98 4 g 0.240 0.280 0.320 0.360 0.400
25 x 117 12 g 0.720 0.840 0.960 1.080 1.200
25 x 165 25 g 1.500 1.750 2.000 2.250 2.500
32 x 169 40 g 2.400 2.800 3.200 3.600 4.000
36 x 237 80 g 4.800 5.600 6.400 7.200 8.000
42 x 256 120 g 7.200 8.400 9.600 10.800 12.000
66 x 195 220 g 13.200 15.400 17.600 19.800 22.000
66 x 268 330 g 19.800 23.100 26.400 29.700 33.000
89 x 382 800 g 48.000 56.000 64.000 72.000 80.000
120 x 429 1 600 g 60.000 70.000 80.000 90.000 100.000

 

Correlation Rf vs CV

Rf CV
0.95 1.05
0.90 1.10
0.85 1.17
0.80 1.25
0.75 1.33
0.70 1.40
0.65 1.54
0.60 1.65
0.55 1.81
0.50 2.00
0.45 2.22
0.40 2.50
0.35 2.86
0.30 3.33
0.25 4.00
0.20 5.00
0.15 6.67
0.10 10.00
0.05 20.00

 

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