SiliaSep Cartridges Characteristics

SiliaSep Flash Cartridge Design

SiliaSep Flash Cartridge design

SiliaSep Features & Benefits

Speed, Reliability & Selectivity
With SiliCycle’s SiliaSep Flash Cartridges you will benefit from the same quality that all our products are known for.
We have the best silica gel available on the market with no fines.

Features	Benefits
Highest silica gel quality with no fines	No product contamination Homogeneous packing, no channelling (no peak tailing) High loading capacity (high surface area) Direct transfer from TLC to flash chromatography (same silica)
Innovative packing technology	Consistent packing for reproducible high plate count (N) Superior performance & separation Higher resolution with improved band definition (no tailing) Greater compound purity & higher recovery
Versatility	Wide choice of cartridge sizes from 4 grams up to 1.5 kg Purification scale-up from milligram to hundreds of grams! Variety of sorbents to address any separation need
Reproducibility, reliability & safety	Leak-free guaranteed by unique one-piece cartridge design Reproducible performance from lot-to-lot (stringent quality control) Excellent durability to withstand high pressures Universal luer fittings for compatibility with any flash systems
Cost effective	Excellent ratio performance vs price Readily available from stock inventory for many volumes

Features

Benefits

Highest silica gel quality with no fines

No product contamination
Homogeneous packing, no channelling (no peak tailing)
High loading capacity (high surface area)
Direct transfer from TLC to flash chromatography (same silica)

Innovative packing technology

Consistent packing for reproducible high plate count (N)
Superior performance & separation
Higher resolution with improved band definition (no tailing)
Greater compound purity & higher recovery

Versatility

Wide choice of cartridge sizes from 4 grams up to 1.5 kg
Purification scale-up from milligram to hundreds of grams!
Variety of sorbents to address any separation need

Reproducibility, reliability & safety

Leak-free guaranteed by unique one-piece cartridge design
Reproducible performance from lot-to-lot (stringent quality control)
Excellent durability to withstand high pressures
Universal luer fittings for compatibility with any flash systems

Cost effective

Excellent ratio performance vs price
Readily available from stock inventory for many volumes

SiliaSep Cartridge Types Overview

Characteristics	Units	SiliaSep 4 g	SiliaSep 12 g	SiliaSep 25 g	SiliaSep 40 g	SiliaSep 80 g
Cartridge Code	-	ISO04	ISO12	ISO25	ISO40	ISO80
Silica weight	g	Bare: 4 g Bonded: ≥ 5 g	Bare: 12 g Bonded: ≥ 15 g	Bare: 25 g Bonded: ≥ 30 g	Bare: 40 g Bonded: ≥ 40 g	Bare: 80 g Bonded: ≥ 90 g
#/box	unit	Bare: 20 Bonded: 2	Bare: 20 Bonded: 1	Bare: 15 Bonded: 1	Bare: 15 Bonded: 1	Bare: 12 Bonded: 1
Dimension (OD x Length)	mm	16 x 98	25 x 117	25 x 165	32 x 169	36 x 237
Column volume	mL	4.9	17	31	47	123
Recom. flow rate	mL/ min	15 - 25	20 - 40	20 - 45	25 - 50	40 - 80
Loading capacity	g	0.005 - 0.4	0.015 - 1.2	0.025 - 2.5	0.05 - 4.0	0.10 - 8.0

Characteristics

Units

SiliaSep 4 g

SiliaSep 12 g

SiliaSep 25 g

SiliaSep 40 g

SiliaSep 80 g

Cartridge Code

ISO04

ISO12

ISO25

ISO40

ISO80

Silica weight

Bare: 4 g
Bonded: ≥ 5 g

Bare: 12 g
Bonded: ≥ 15 g

Bare: 25 g
Bonded: ≥ 30 g

Bare: 40 g
Bonded: ≥ 40 g

Bare: 80 g
Bonded: ≥ 90 g

#/box

unit

Bare: 20
Bonded: 2

Bare: 20
Bonded: 1

Bare: 15
Bonded: 1

Bare: 12
Bonded: 1

Dimension
(OD x Length)

16 x 98

25 x 117

25 x 165

32 x 169

36 x 237

Column volume

4.9

123

Recom. flow rate

mL/ min

15 - 25

20 - 40

20 - 45

25 - 50

40 - 80

Loading capacity

0.005 - 0.4

0.015 - 1.2

0.025 - 2.5

0.05 - 4.0

0.10 - 8.0

Characteristics	Units	SiliaSep 120 g	SiliaSep 220 g	SiliaSep 330 g	SiliaSep XL 800 g	SiliaSep XL 1600 g
Cartridge Code	-	IS120	IS220	IS330	IS750	I1500
Silica weight	g	Bare: 120 g Bonded: ≥ 130 g	Bare: 220 g Bonded: ≥ 230 g	Bare: 330 g Bonded: ≥ 360 g	Bare: 800 g Bonded: ≥ 870 g	Bare: 1,600 g Bonded: ≥ 1,700 g
#/box	unit	Bare: 10 Bonded: 1	Bare: 4 Bonded: 1	Bare: 4 Bonded: 1	Bare: 2 Bonded: 1	Bare: 2 Bonded: 1
Dimension (OD x Length)	mm	42 x 256	66 x 195	66 x 268	89 x 382	104 x 429
Column volume	mL	190	306	441	1,500	2,900
Recom. flow rate	mL/ min	60 - 120	60 - 180	80 - 180	200 - 300	300 - 450
Loading capacity	g	0.15 - 12.0	0.25 - 22.0	0.50 - 33.0	0.80 - 80.0	1.60 - 160.0

Characteristics

Units

SiliaSep 120 g

SiliaSep 220 g

SiliaSep 330 g

SiliaSep XL 800 g

SiliaSep XL 1600 g

Cartridge Code

IS120

IS220

IS330

IS750

I1500

Silica weight

Bare: 120 g
Bonded: ≥ 130 g

Bare: 220 g
Bonded: ≥ 230 g

Bare: 330 g
Bonded: ≥ 360 g

Bare: 800 g
Bonded: ≥ 870 g

Bare: 1,600 g
Bonded: ≥ 1,700 g

#/box

unit

Bare: 10
Bonded: 1

Bare: 4
Bonded: 1

Bare: 2
Bonded: 1

Dimension
(OD x Length)

42 x 256

66 x 195

66 x 268

89 x 382

104 x 429

Column volume

190

306

441

1,500

2,900

Recom. flow rate

mL/ min

60 - 120

60 - 180

80 - 180

200 - 300

300 - 450

Loading capacity

0.15 - 12.0

0.25 - 22.0

0.50 - 33.0

0.80 - 80.0

1.60 - 160.0

How to create product number:
Product Number => FLH - [Chemistry Code] - [Cartridge Code] Ex. cartridge 4 g with silica gel => FLH-R10030B-ISO04

SiliaSep Loading Chart

Dimensions OD x L (mm)	SiliaSep Format	ΔCV = 0.1-0.6 Load (g)	ΔCV = 0.7-1.2 Load (g)	ΔCV = 1.3-1.8 Load (g)	ΔCV = 1.9-2.4 Load (g)	ΔCV = 2.5-3.1 Load (g)
16 x 98	4 g	0.040	0.080	0.120	0.160	0.200
25 x 117	12 g	0.120	0.240	0.360	0.480	0.600
25 x 165	25 g	0.250	0.500	0.750	1.000	1.250
32 x 169	40 g	0.400	0.800	1.200	1.600	2.000
36 x 237	80 g	0.800	1.600	2.400	3.200	4.000
42 x 256	120 g	1.200	2.400	3.600	4.800	6.000
66 x 195	220 g	2.200	4.400	6.600	8.800	11.000
66 x 268	330 g	3.300	6.600	9.900	13.200	16.500
89 x 382	800 g	8.000	16.000	24.000	32.000	40.000
120 x 429	1 600 g	10.000	20.000	30.000	40.000	50.000

Dimensions OD x L (mm)	SiliaSep Format	ΔCV = 3.2-3.8 Load (g)	ΔCV = 3.9-4.5 Load (g)	ΔCV = 4.6-5.2 Load (g)	ΔCV = 5.3-6.0 Load (g)	ΔCV = > 6 Load (g)
16 x 98	4 g	0.240	0.280	0.320	0.360	0.400
25 x 117	12 g	0.720	0.840	0.960	1.080	1.200
25 x 165	25 g	1.500	1.750	2.000	2.250	2.500
32 x 169	40 g	2.400	2.800	3.200	3.600	4.000
36 x 237	80 g	4.800	5.600	6.400	7.200	8.000
42 x 256	120 g	7.200	8.400	9.600	10.800	12.000
66 x 195	220 g	13.200	15.400	17.600	19.800	22.000
66 x 268	330 g	19.800	23.100	26.400	29.700	33.000
89 x 382	800 g	48.000	56.000	64.000	72.000	80.000
120 x 429	1 600 g	60.000	70.000	80.000	90.000	100.000

Correlation Rf vs CV

Rf	CV
0.95	1.05
0.90	1.10
0.85	1.17
0.80	1.25
0.75	1.33
0.70	1.40
0.65	1.54
0.60	1.65
0.55	1.81
0.50	2.00
0.45	2.22
0.40	2.50
0.35	2.86
0.30	3.33
0.25	4.00
0.20	5.00
0.15	6.67
0.10	10.00
0.05	20.00

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