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SiliaSep Flash Cartridges - Normal Phases

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Normal phase chromatography is used to separate polar compounds through polar interactions with the support. The interactions take place on the highly polar silanols of the silica gel surface, but there are also moderately polar interactions with the hydrogen bonds on Amino or Diol functions.

Our pre-packed normal phases cartridges are available in two silica gel grades:

  • SiliaSep, irregular 40-63 microns silica
  • SiliaSep PREMIUM, spherical 25 microns silica
SiliaSep Type Description
SiliaSep PREMIUM Silica
FLH-10095D-A		 Most popular sorbent for day-to-day use for the purification of non-ionic polar organic compounds.
SiliaSep Silica
FLH-R10030B
SiliaSep PREMIUM Amine
FLH-52095D-A		 Good alternative for normal phase purification of compounds with basic properties. Useful for monosaccharides separation.
Note: imine formation can be seen with the purification of aldehydes and ketones.
SiliaSep Amine
FLH-R52030B
SiliaSep PREMIUM Cyano
FLH-38095D-A		 Versatile sorbent that can be used either as normal or reversed-phase. Less polar sorbent compared to silica. Used for the purification of polar organic compounds.
SiliaSep Cyano
FLH-R38030B
SiliaSep PREMIUM Diol nec
FLH-35095D-A		 Good alternative for difficult separation of low to medium polarity samples. Useful for mono and polysaccharides separation. Can be used in HILIC mode.
SiliaSep Diol nec
FLH-R35030B
SiliaSep Acidic Alumina
FLH-AUT-0053		 Good retention of aromatic compounds, aliphatic amines and compounds containing electronegative functions.
SiliaSep Neutral Alumina
FLH-AUT-0054
SiliaSep Basic Alumina
FLH-AUT-0055
SiliaSep Silver Nitrate (AgNO3)
FLH-R23530B		 Mainly used for the separation of cis / trans isomers of unsaturated compounds such as alkenes, lipids, steroids and terpenes.

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  1. SiliaSep Flash Cartridges, Silica, 25 µm (FLH-10095D-A)

    SiliaSep PREMIUM Flash Cartridges, Silica, spherical 25 µm (FLH-10095D-A)

    Most popular sorbent for day-to-day use for the purification of non-ionic polar organic compounds.
  2. SiliaSep PREMIUM Flash Cartridges, Cyano (CN), 25 µm, 90 Å (FLH-38095D-A)

    SiliaSep PREMIUM Flash Cartridges, Cyano (CN), 25 µm, 90 Å (FLH-38095D-A)

    Versatile sorbent that can be used either as normal or reversed-phase. Indicated for products with intermediate to extreme polarity. The slightly hydrophobic nature of the cyano group offers alternative selectivity.
  3. SiliaSep Flash Cartridges, Silica, 40 - 63 µm, 60 Å (FLH-R10030B)

    SiliaSep Flash Cartridges, Silica, 40 - 63 µm, 60 Å (FLH-R10030B)

    Most popular sorbent for day-to-day use for the purification of non-ionic polar organic compounds.
  4. SiliaSep Flash Cartridges, Amine (WAX), 25 µm (FLH-52095D-A)

    SiliaSep PREMIUM Flash Cartridges, Amine (WAX), spherical 25 µm (FLH-52095D-A)

    The amine phase can be used either in normal phase for the purification of compounds with basic properties & for monosaccharides separation; or as a weak anion exchanger (pKa of 9.8). At pH 7.8 or below, the functional groups are positively charged. It facilitates the rapid release of very strong anions such as sulfonic acids that may be too strongly retained on SAX.

  5. SiliaSep Flash Cartridges, Amine (WAX), 40 - 63 µm, 60 Å (FLH-R52030B)

    SiliaSep Flash Cartridges, Amine (WAX), 40 - 63 µm, 60 Å (FLH-R52030B)

    Good alternative for normal phase purification of compounds with basic properties, which would normally have to be purified by reversed-phase. Also a weak anion exchanger, with a pKa of 9.8. At pH 7.8 or below, the functional groups are positively charged. It facilitates the rapid release of very strong anions such as sulfonic acids that may be too strongly retained on SAX.
  6. SiliaSep Flash Cartridges, Cyano (CN), 40 - 63 µm, 60 Å (FLH-R38030B)

    SiliaSep Flash Cartridges, Cyano (CN), 40 - 63 µm, 60 Å (FLH-R38030B)

    Versatile sorbent that can be used either as normal or reversed-phase. Indicated for products with intermediate to extreme polarity. The slightly hydrophobic nature of the cyano group offers alternative selectivity.
  7. SiliaSep Flash Cartridges, Diol, 25 µm (FLH-35095D-A)

    SiliaSep PREMIUM Flash Cartridges, Diol, spherical 25 µm (FLH-35095D-A)

    Good alternative for difficult separation of low to medium polarity samples. Useful for mono and polysaccharides separation. Can be used in HILIC mode.
  8. SiliaSep Flash Cartridges, Diol nec, 40 - 63 µm, 60 Å (FLH-R35030B)

    SiliaSep Flash Cartridges, Diol nec, 40 - 63 µm, 60 Å (FLH-R35030B)

    Good alternative for difficult separation of low to medium polarity samples. Useful for mono and polysaccharides separation. Can be used in HILIC mode.
  9. SiliaSep Flash Cartridges, Acidic Alumina, 50 - 200 µm, 60 Å (FLH-AUT-0053)

    SiliaSep Flash Cartridges, Acidic Alumina, 50 - 75 µm, 60 Å (FLH-AUT-0053)

    Good retention of aromatic compounds, aliphatic amines and compounds containing electronegative functions.
  10. SiliaSep Flash Cartridges, Neutral Alumina, 50 - 200 µm, 60 Å (FLH-AUT-0054)

    SiliaSep Flash Cartridges, Neutral Alumina, 50 - 75 µm, 60 Å (FLH-AUT-0054)

    Good retention of aromatic compounds, aliphatic amines and compounds containing electronegative functions.
  11. SiliaSep Flash Cartridges, Basic Alumina, 50 - 200 µm, 60 Å (FLH-AUT-0055)

    SiliaSep Flash Cartridges, Basic Alumina, 50 - 75 µm, 60 Å (FLH-AUT-0055)

    Good retention of aromatic compounds, aliphatic amines and compounds containing electronegative functions.
  12. SiliaSep Flash Cartridges, Silver Nitrate (AgNO3), 40 - 63 µm, 60 Å (FLH-R23530B)

    SiliaSep Flash Cartridges, Silver Nitrate (AgNO3), 40 - 63 µm, 60 Å (FLH-R23530B)

    Mainly used for the separation of cis / trans isomers of unsaturated compounds such as alkenes, lipids, steroids and terpenes.

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Support Backbone
  1. Silica Gels (12)
Family / Formats
  1. SiliaSep™ (Flash Cartridges) (10)
  2. SiliaSep™ PREMIUM (Flash Cartridges) (4)
Array
  1. Alumina (3)
  2. Amine (WAX) (2)
  3. AQ C18 (1)
  4. Cyano (2)
  5. Diol (2)
  6. Silica (2)
  7. Silver Nitrate (AgNO3) (1)
Pore Size
  1. 60 Å (8)
  2. 90 Å (4)
Endcapping
  1. Endcapped (6)
  2. Non-endcapped (5)
Cartridges Volume
  1. 4.9 mL (1)
  2. 17 mL (1)
  3. 25 mL (1)
  4. 31 mL (1)
  5. 47 mL (1)
  6. 123 mL (1)
  7. 190 mL (1)
  8. 306 mL (1)
  9. 441 mL (1)
  10. 1 500 mL (1)
  11. 2 900 mL (1)
Bed Weight
  1. 4 g (12)
  2. 5 g (1)
  3. 12 g (12)
  4. 25 g (12)
  5. 40 g (12)
  6. 80 g (12)
  7. 120 g (12)
  8. 220 g (12)
  9. 330 g (12)
  10. 800 g (11)
  11. 1600 g (11)
Particle Size
  1. 25 µm (4)
  2. 40 - 63 µm / 230 - 400 mesh (5)
  3. 50 - 200 µm / 75 - 300 mesh (3)
  4. 50 - 75 µm / 200 - 300 mesh (3)

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SiliaSep PREMIUM - Flash Cartridges packed with Spherical Silica
E-PAK® - Fixed Bed Flow-through Purification Cartridges

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