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SiliaSep Flash Cartridges - Reversed-phases

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In reversed-phase chromatography, the packing material is always non-polar (hydrophobic) while the mobile phase is polar to non-polar. An important parameter affecting chromatographic efficiency is the hydrophobicity of the sorbent. As a general rule, stationary phase hydrophobicity increases with the alkyl chain length.

Our pre-packed reversed-phases cartridges are available in two silica gel grades:

  • SiliaSep, irregular 40-63 microns silica
  • SiliaSep PREMIUM, spherical 25 microns silica

SiliaSep Type Description
SiliaSep PREMIUM C18
FLH-03295D-A
Indicated for the purification of low to high polarity compounds. Provides reproducible purification without the complexity and cost of preparative HPLC.
SiliaSep C18 (17%)
FLH-R33230B
SiliaSep PREMIUM C8
FLH-30895D-A
Presents less retention compared to C18. Typically used for highly hydrophobic pesticides, small peptides and large molecule drugs.
SiliaSep C8
FLH-R30830B
SiliaSep PREMIUM C4
FLH-32095D-A
Presents less retention compared to C18 and C8. Widely used for molecules with large hydrophobic regions.
SiliaSep C4
FLH-R32030B
SiliaSep PREMIUM Phenyl
FLH-34095D-A
Moderate non-polar sorbent with different selectivity for aromatic compounds compared to other non-polar sorbents.
SiliaSep Phenyl
FLH-R34030B
SiliaSep PREMIUM PFP
FLH-67595D-A
For a new selectivity approach with aromatic ring interactions, or for the purification of conjugated compounds (isomers).
SiliaSep PFP
FLH-R67530B
SiliaSep PREMIUM Cyano
FLH-38095D-A
Versatile sorbent that can be used either as normal or reversed-phase. Indicated for products with intermediate to extreme polarity. The slightly hydrophobic nature of the cyano group offers alternative selectivity.
SiliaSep Cyano
FLH-R38030B

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  1. SiliaSep PREMIUM Flash Cartridges, C18 Monomeric, 25 µm (FLH-03295D-A)

    SiliaSep PREMIUM Flash Cartridges, C18, spherical 25 µm (FLH-03295D-A)

    Indicated for the purification of low to high polarity compounds. Provides reproducible purification without the complexity and cost of preparative HPLC.
  2. SiliaSep PREMIUM Flash Cartridges, C4, spherical 25 µm (FLH-32095D-A)

    SiliaSep PREMIUM Flash Cartridges, C4, spherical, 25 µm, 90 Å (FLH-32095D-A)

    Presents less retention compared to C18 and C8. Widely used for molecules with large hydrophobic regions.
  3. SiliaSep PREMIUM Flash Cartridges, Phenyl, spherical, 25 µm, 90 Å (FLH-34095D-A)

    SiliaSep PREMIUM Flash Cartridges, Phenyl, spherical, 25 µm, 90 Å (FLH-34095D-A)

    Moderate non-polar sorbent with different selectivity for aromatic compounds compared to other non-polar sorbents.
  4. SiliaSep PREMIUM Flash Cartridges, Pentafluorophenyl (PFP), 25 µm, 90 Å (FLH-67595D-A)

    SiliaSep PREMIUM Flash Cartridges, Pentafluorophenyl (PFP), 25 µm, 90 Å (FLH-67595D-A)

    For a new selectivity approach with aromatic ring interactions, or for the purification of conjugated compounds (isomers).
  5. SiliaSep PREMIUM Flash Cartridges, Cyano (CN), 25 µm, 90 Å (FLH-38095D-A)

    SiliaSep PREMIUM Flash Cartridges, Cyano (CN), 25 µm, 90 Å (FLH-38095D-A)

    Versatile sorbent that can be used either as normal or reversed-phase. Indicated for products with intermediate to extreme polarity. The slightly hydrophobic nature of the cyano group offers alternative selectivity.
  6. SiliaSep Flash Cartridges, C18 (17%) monomeric, 40 - 63 µm, 60 Å (FLH-R33230B)

    SiliaSep Flash Cartridges, C18 (17%), 40 - 63 µm, 60 Å (FLH-R33230B)

    Indicated for the purification of low to high polarity compounds. Provides reproducible purification without the complexity and cost of preparative HPLC.
  7. SiliaSep Flash Cartridges, C8 Monomeric, 25 µm (FLH-30895D-A)

    SiliaSep PREMIUM Flash Cartridges, C8, spherical 25 µm (FLH-30895D-A)

    Presents less retention compared to C18. Typically used for highly hydrophobic pesticides, small peptides and large molecule drugs.
  8. SiliaSep Flash Cartridges, C8 monomeric, 40 - 63 µm, 60 Å (FLH-R30830B)

    SiliaSep Flash Cartridges, C8, 40 - 63 µm, 60 Å (FLH-R30830B)

    Presents less retention compared to C18. Typically used for highly hydrophobic pesticides, small peptides and large molecule drugs.
  9. SiliaSep Flash Cartridges, C8 nec, 40 - 63 µm, 60 Å (FLH-R31130B)

    SiliaSep Flash Cartridges, C8 nec, 40 - 63 µm, 60 Å (FLH-R31130B)

    Presents less retention compared to C18. Typically used for highly hydrophobic pesticides, small peptides and large molecule drugs.
  10. SiliaSep Flash Cartridges, C4, 40 - 63 µm, 60 Å (FLH-R32030B)

    SiliaSep Flash Cartridges, C4, 40 - 63 µm, 60 Å (FLH-R32030B)

    Presents less retention compared to C18 and C8. Widely used for molecules with large hydrophobic regions.
  11. SiliaSep Flash Cartridges, Phenyl, 40 - 63 µm, 60 Å (FLH-R34030B)

    SiliaSep Flash Cartridges, Phenyl, 40 - 63 µm, 60 Å (FLH-R34030B)

    Moderate non-polar sorbent with different selectivity for aromatic compounds compared to other non-polar sorbents.
  12. SiliaSep Flash Cartridges, Phenyl nec, 40 - 63 µm, 60 Å (FLH-R34130B)

    SiliaSep Flash Cartridges, Phenyl nec, 40 - 63 µm, 60 Å (FLH-R34130B)

    Moderate non-polar sorbent with different selectivity for aromatic compounds compared to other non-polar sorbents.
  13. SiliaSep Flash Cartridges, Pentafluorophenyl, 40 - 63 µm, 60 Å (FLH-R67530B)

    SiliaSep Flash Cartridges, Pentafluorophenyl (PFP), 40 - 63 µm, 60 Å (FLH-R67530B)

    For a new selectivity approach with aromatic ring interactions, or for the purification of conjugated compounds (isomers).
  14. SiliaSep Flash Cartridges, Cyano (CN), 40 - 63 µm, 60 Å (FLH-R38030B)

    SiliaSep Flash Cartridges, Cyano (CN), 40 - 63 µm, 60 Å (FLH-R38030B)

    Versatile sorbent that can be used either as normal or reversed-phase. Indicated for products with intermediate to extreme polarity. The slightly hydrophobic nature of the cyano group offers alternative selectivity.

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