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SiliaSep Flash Cartridges - Reversed-phases

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In reversed-phase chromatography, the packing material is always non-polar (hydrophobic) while the mobile phase is polar to non-polar. An important parameter affecting chromatographic efficiency is the hydrophobicity of the sorbent. As a general rule, stationary phase hydrophobicity increases with the alkyl chain length.

SiliaSep Type Description
SiliaSep C18
FLH-R33230B
Indicated for the purification of low to high polarity compounds. Provides reproducible purification without the complexity and cost of preparative HPLC.
SiliaSep C8
FLH-R30830B
Presents less retention compared to C18. Typically used for highly hydrophobic pesticides, small peptides and large molecule drugs.
SiliaSep C4
FLH-R32030B
Presents less retention compared to C18 and C8. Widely used for molecules with large hydrophobic regions.
SiliaSep Phenyl
FLH-R34030B
Moderate non-polar sorbent with different selectivity for aromatic compounds compared to other non-polar sorbents.
SiliaSep Pentafluorophenyl (PFP)
FLH-R67530B
For a new selectivity approach with aromatic ring interactions, or for the purification of conjugated compounds (isomers).
SiliaSep Cyano
FLH-R38030B
Versatile sorbent that can be used either as normal or reversed-phase. Indicated for products with intermediate to extreme polarity. The slightly hydrophobic nature of the cyano group offers alternative selectivity.

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  1. SiliaSep Flash Cartridges, C18 Monomeric, 4 g, 20 - 45 µm, 300 Å (FLH-03220M)
  2. SiliaSep Flash Cartridges, C8 Monomeric, 25 µm (FLH-30895D-A)
  3. SiliaSep Flash Cartridges, C18 (17%) monomeric, 40 - 63 µm, 60 Å (FLH-R33230B)

    SiliaSep Flash Cartridges, C18 (17%) monomeric, 40 - 63 µm, 60 Å (FLH-R33230B)

    Indicated for the purification of low to high polarity compounds. Provides reproducible purification without the complexity and cost of preparative HPLC.
  4. SiliaSep Flash Cartridges, C8 monomeric, 40 - 63 µm, 60 Å (FLH-R30830B)

    SiliaSep Flash Cartridges, C8 monomeric, 40 - 63 µm, 60 Å (FLH-R30830B)

    Presents less retention compared to C18. Typically used for highly hydrophobic pesticides, small peptides and large molecule drugs.
  5. SiliaSep Flash Cartridges, C8 nec, 40 - 63 µm, 60 Å (FLH-R31130B)

    SiliaSep Flash Cartridges, C8 nec, 40 - 63 µm, 60 Å (FLH-R31130B)

    Presents less retention compared to C18. Typically used for highly hydrophobic pesticides, small peptides and large molecule drugs.
  6. SiliaSep Flash Cartridges, C4, 40 - 63 µm, 60 Å (FLH-R32030B)

    SiliaSep Flash Cartridges, C4, 40 - 63 µm, 60 Å (FLH-R32030B)

    Presents less retention compared to C18 and C8. Widely used for molecules with large hydrophobic regions.
  7. SiliaSep Flash Cartridges, Phenyl, 40 - 63 µm, 60 Å (FLH-R34030B)

    SiliaSep Flash Cartridges, Phenyl, 40 - 63 µm, 60 Å (FLH-R34030B)

    Moderate non-polar sorbent with different selectivity for aromatic compounds compared to other non-polar sorbents.
  8. SiliaSep Flash Cartridges, Phenyl nec, 40 - 63 µm, 60 Å (FLH-R34130B)

    SiliaSep Flash Cartridges, Phenyl nec, 40 - 63 µm, 60 Å (FLH-R34130B)

    Moderate non-polar sorbent with different selectivity for aromatic compounds compared to other non-polar sorbents.
  9. SiliaSep Flash Cartridges, Pentafluorophenyl, 40 - 63 µm, 60 Å (FLH-R67530B)

    SiliaSep Flash Cartridges, Pentafluorophenyl (PFP), 40 - 63 µm, 60 Å (FLH-R67530B)

    For a new selectivity approach with aromatic ring interactions, or for the purification of conjugated compounds (isomers).
  10. SiliaSep Flash Cartridges, Cyano (CN), 40 - 63 µm, 60 Å (FLH-R38030B)

    SiliaSep Flash Cartridges, Cyano (CN), 40 - 63 µm, 60 Å (FLH-R38030B)

    Versatile sorbent that can be used either as normal or reversed-phase. Indicated for products with intermediate to extreme polarity. The slightly hydrophobic nature of the cyano group offers alternative selectivity.

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