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SiliaSep Flash Cartridges

SiliaSep Flash Cartridges Portfolio

SiliaSep - Normal Phases

Normal phase chromatography is used to separate polar compounds through polar interactions with the support. The interactions take place on the highly polar silanols of the silica gel surface, but there are also moderately polar interactions with the hydrogen bonds on Amino or Diol functions.

SiliaSep Type PN Description
SiliaSep Silica FLH-R10030B Most popular sorbent for day-to-day use for the purification of non-ionic polar organic compounds.
SiliaSep HP Silica FLH-R10017B High Performance sorbent for difficult separations (isomers). Higher loading capacity. Faster flow rate. Less solvent used.
SiliaSep Amine FLH-R52030B Good alternative for normal phase purification of compounds with basic properties. Useful for monosaccharides separation.
Note: imine formation can be seen with the purification of aldehydes and ketones.
SiliaSep Cyano FLH-R38030B Versatile sorbent that can be used either as normal or reversed-phase. Less polar sorbent compared to silica. Used for the purification of polar organic compounds.
SiliaSep Diol nec FLH-R35030B Good alternative for difficult separation of low to medium polarity samples. Useful for mono and polysaccharides separation. Can be used in HILIC mode.
SiliaSep Neutral Alumina FLH-AUT-0054 Good retention of aromatic compounds, aliphatic amines and compounds containing electronegative functions.
SiliaSep Basic Alumina FLH-AUT-0055
SiliaSep Silver Nitrate (AgNO3) FLH-R23530B Mainly used for the separation of cis / trans isomers of unsaturated compounds such as alkenes, lipids, steroids and terpenes.

SiliaSep - Reversed Phases

SiliaSep Type PN Description
SiliaSep C18 FLH-R33230B Indicated for the purification of low to high polarity compounds. Provides reproducible purification without the complexity and cost of preparative HPLC.
SiliaSep C8 FLH-R30830B Presents less retention compared to C18. Typically used for highly hydrophobic pesticides, small peptides and large molecule drugs.
SiliaSep C8 nec FLH-R31130B
SiliaSep C4 FLH-R32030B Presents less retention compared to C18 and C8. Widely used for molecules with large hydrophobic regions.
SiliaSep Phenyl (PH) FLH-R34030B Moderate non-polar sorbent with different selectivity for aromatic compounds compared to other non-polar sorbents.
SiliaSep Phenyl nec (PH) FLH-R34130B
SiliaSep Pentafluorophenyl (PFP) FLH-R67530B For a new selectivity approach with aromatic ring interactions, or for the purification of conjugated compounds (isomers).
SiliaSep Cyano FLH-R38030B Versatile sorbent that can be used either as normal or reversed-phase. Indicated for products with intermediate to extreme polarity. The slightly hydrophobic nature of the cyano group offers alternative selectivity.

SiliaSep - Ion Exchange Phases

In ion exchange mode, the silica support is modified by a function carrying a charge with its counter-ion. This counter-ion is exchangeable with other ions in solution. If the immobilized phase is carrying an anion, the exchangeable species is a cation. Inversely, if the immobilized phase carries a cation, the ion exchangeable species will be an anion. Ion exchange phases are widely used in separation and purification.

SiliaSep Type PN Description
SiliaSep SAX nec
(TMA Chloride)
FLH-R66530B The quaternary amine is permanently charged (pH independent). It is commonly used for the extraction of weak anions (such as carboxylic acids) that may not bind strongly enough to weaker anion exchangers. Analysis of acidic drugs and analgesics, biomolecules (peptides and proteins) and water-soluble vitamins (vitamins B and C).
SiliaSep SAX-2 nec
(TMA Acetate)
FLH-R66430B The acetate counter-ion is easily exchangeable (more than the chloride ion) for compounds with pKa < 5, such as carboxylic acids. This phase can be used in organic chemistry applications to selectively purify acidic compounds or remove acidic impurities from reaction mixtures.
SiliaSep WAX nec
(Amine)
FLH-R52030B A weak anion exchanger with a pKa of 9.8. At pH 7.8 or below, the functional groups are positively charged. It facilitates the rapid release of very strong anions such as sulfonic acids that may be too strongly retained on SAX.
SiliaSep Diamine FLH-R49030B
SiliaSep Triamine FLH-R48030B
SiliaSep WAX-2
(Diethylamine)
FLH-R76530B With a pKa of 10.5, this phase is prefered over SAX when performing catch and release purification of compounds bearing a permanent negative charge such as salts of sulfonic acids. Using SAX in this case could make the release of the compound of interest difficult (but not necessarily impossible) due to the strong interaction between the two strong ions.
SiliaSep SCX
(Tosic Acid)
FLH-R60530B Due to the very low pKa (< 1) these functions are strong cation exchangers since they maintain a negative charge throughout the pH scale. The most common use is likely for catch and release purification of weak cations (for example basic drugs and analgesics), analysis of basic biomolecules (peptides and proteins) and water-soluble vitamins (basic vitamins B and C).
SiliaSep SCX-2
(Propylsulfonic Acid)
FLH-R51230B
SiliaSep Bicarbonate FLH-R65830B

SiliaSep - Metal Scavengers

Lower the residual metal concentration of various metal complexes to single digit ppm with SiliaSep Metal Scavengers. Any synthetic pathway is now possible, even the ones using metal catalysts.

SiliaSep Type PN Description
SiliaSep Thiol FLH-R51030B Thiol is our most versatile and robust metal scavenger for a variety of metals under a wide range of conditions.

Best scavenger for: Ag, Hg, Os, Pd & Ru
Good scavenger for: Cu, Ir, Pb, Rh & Sn
SiliaSep TAAcOH FLH-R69030B TAAcOH (Triaminetetraacetic Acid) is a silica bound metal scavenger for Pd0, Ni0 and Cu. It is the supported version of EDTA in its free form. It is an effective scavenger for metals in low or zero oxidation states, which includes many of the most synthetically useful catalysts such as tetrakis-(triphenylphosphine)palladium0.

Best scavenger for: Ca, Co, Ir, Li, Mg, Ni, Os, Ru & Sc
Good scavenger for: Cr, Cs, Fe, Pd, Rh & Sn
SiliaSep TAAcONa FLH-R69230B TAAcONa (Triaminetetraacetate, sodium salt) is a silica bound metal scavenger for Pd(II), Ni(II) and Cu. It is the supported version of EDTA in its sodium salt form. It is an effective scavenger for metals in higher oxidation state, 2+ or higher.

Best scavenger for: Ca, Cd, Cs, Cu, Fe, Ir, La, Li, Mg, Ni, Os, Rh, Sc & Sn
Good scavenger for: Cr, Pd, Ru, Se & Zn
SiliaSep Thiourea FLH-R69530B Thiourea is a versatile metal scavenger for all forms of palladium and is widely used in the pharmaceutical industry. It works particularly well in organic solvents. Once complexed with a transition metal, it has been reported as being an effective catalyst.

Best scavenger for: Pd & Ru
Good scavenger for: Ag, Cu, Fe, Os, Rh, Sc & Sn
SiliaSep DMT FLH-R79030B DMT is the silica-bound equivalent of 2,4,6 - trimercaptotriazine (trithiocyanuric acid, TMT). It is a versatile metal scavenger for a variety of metals and the preferred metal scavenger for ruthenium catalysts and hindered Pd complexes (i.e. Pd(dppf)Cl2).

Best scavenger for: Ir, Ni, Os, Pd, Pt, Rh, Se & Ru
Good scavenger for: Cd, Co, Cu, Fe, Sc & Zn
SiliaSep Imidazole FLH-R79230B Imidazole is a versatile metal scavenger for a variety of metals, under a wide range of conditions, and the preferred metal scavenger for iron catalysts.
Imidazole can also be used as an amine base in systems that are generally sensitive to basic conditions compared to other SiliaMetS supported bases.

Best scavenger for: Cd, Co, Cu, Fe, Ir, Li, Mg, Ni, Os, W & Zn
Good scavenger for: Cr, Pd & Rh
SiliaSep Cysteine FLH-R80530B Cysteine is the silica bound equivalent of the amino acid Cysteine. By attaching the molecule to the backbone via the amino group, the thiol group remains free and accessible for higher metal scavenging efficiency. It is a versatile metal scavenger for a variety of metals, under a wide range of conditions, and the preferred metal scavenger for tin residues.

Best scavenger for: Cd, Fe, Ir, Os, Ru, Sc & Sn
Good scavenger for: Ca, Cr, Cs, Cu, La, Mg, Pd, Pt, Rh, Se & Zn
SiliaSep Diamine FLH-R49030B Also known for its electrophile scavenging efficiency and its base reagent quality, Diamine has also proven to be very useful for the scavenging of various metals.

Best scavenger for: Cr, Pd, Pt, W & Zn
Good scavenger for: Cd, Co, Cu, Fe, Hg, Ni, Pb, Ru & Sc
SiliaSep Triamine FLH-R48030B Also known for its electrophile scavenging efficiency and its base reagent quality, Triamine has also proven to be very useful for the scavenging of various metals.

Best scavenger for: Cr, Pd, Pt, W & Zn
Good scavenger for: Ag, Cd, Co, Cu, Fe, Hg, Ni, Os, Pb, Rh, Ru & Sc