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SiliaSep Reversed-Phase

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In reversed-phase chromatography, the packing material is always non-polar (hydrophobic) while the mobile phase is polar to non-polar. An important parameter affecting chromatographic efficiency is the hydrophobicity of the sorbent. As a general rule, stationary phase hydrophobicity increases with the alkyl chain length.

SiliCycle developed a C18 chromatographic phase (PN: R33230B) characterized by a homogeneous coverage of the alkyl chains on the surface. Consequently, the endcapping step is more controlled, which leads better separations and inhibition of the non-specific interactions with silanol groups (highly deactivated silanol phase).

Compared to competitive products, this endcapped C18 phase with 17 % carbon load exhibits high hydrophobicity and base deactivated properties. We have compared this high-performance chromatographic phase to similar C18 phases on the market (20 % carbon load). The comparison was done on a mixture of compounds to evaluate the dead volume (uracil), the hydrophobicity (toluene and biphenyl) and the silanol activity (amitriptyline). The test was done in isocratic conditions, with a mobile phase composed of 80/20 methanol / buffer (20 nM potassium phosphate pH = 7).

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  1. SiliaSep Flash Cartridges, C18, 40-63 µm (230-400 mesh), 60 Å (FLH-R33230B)

    SiliaSep Flash Cartridges, C18, 40-63 µm, 60 Å (FLH-R33230B)

    Indicated for the purification of medium to high polarity compounds. Provide reproducible purification without the complexity and cost of preparative HPLC.
  2. SiliaSep Flash Cartridges, Cyano, 40-63 µm, 60 Å (FLH-R38030B)

    SiliaSep Flash Cartridges, Cyano, 40-63 µm, 60 Å (FLH-R38030B)

    Versatile sorbent that can be used either as normal or reversed-phase. Indicated for products with intermediate to extreme polarity. The slightly hydrophobic nature of the cyano group offers alternative selectivity.
  3. SiliaSep Flash Cartridges, Pentafluorophenyl (PFP), 40-63 µm (230-400 mesh), 60 Å (FLH-R67530B)

    SiliaSep Flash Cartridges, Pentafluorophenyl (PFP), 40-63 µm, 60 Å (FLH-R67530B)

    For a new selectivity approach with aromatic ring interactions, or for the purification of conjugated compounds (isomers).
  4. SiliaSep Flash Cartridges, Phenyl (PH), 40-63 µm, 60 Å (FLH-R34030B)

    SiliaSep Flash Cartridges, Phenyl (PH), 40-63 µm, 60 Å (FLH-R34030B)

    Moderate non-polar sorbent with different selectivity for aromatic compounds compared to other non-polar sorbents.
  5. SiliaSep Flash Cartridges, Phenyl (PH) nec, 40-63 µm, 60 Å (FLH-R34130B)

    SiliaSep Flash Cartridges, Phenyl nec, 40-63 µm, 60 Å (FLH-R34130B)

    Moderate non-polar sorbent with different selectivity for aromatic compounds compared to other non-polar sorbents.
  6. SiliaSep Flash Cartridges, C4, 40-63 µm, 60 Å (FLH-R32030B)

    SiliaSep Flash Cartridges, C4, 40-63 µm, 60 Å (FLH-R32030B)

    Presents less retention compared to C18 and C8. Widely used for molecules with large hydrophobic regions.
  7. SiliaSep Flash Cartridges, C8, 40-63 µm, 60 Å (FLH-R30830B)

    SiliaSep Flash Cartridges, C8, 40-63 µm, 60 Å (FLH-R30830B)

    Presents less retention compared to C18. Typically used for highly hydrophobic pesticides, small peptides and large molecule drugs.
  8. SiliaSep Flash Cartridges, C8 nec, 40-63 µm, 60 Å (FLH-R31130B)

    SiliaSep Flash Cartridges, C8 nec, 40-63 µm, 60 Å (FLH-R31130B)

    Presents less retention compared to C18. Typically used for highly hydrophobic pesticides, small peptides and large molecule drugs.

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