SiliaPrep Tips Sorbent Selection Guide
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The chart below will help you choose the right sorbent for your application.
| Molecule | Application | Analyte | Sorbent |
|---|---|---|---|
| Small Molecules | Desalting | All | C18; Carbon Black |
| Protein removal | All | C18; HILIC | |
| Metal scavenging | All | Cysteine; DMT; Imidazole; PSA; TAAcOH; TAAcONa; Thiol; Thiourea; Triamine | |
| Enrichment | Hydrophobic | C18; HLB; DVB; Carbon Black; HILIC | |
| Hydrophilic | Silica; Cyano; Carbon Black; HILIC | ||
| Neutral | C18; HLB; DVB; Carbon Black; HILIC; Cyano | ||
| Cationic | SCX; WCX; Polymeric SCX & WCX | ||
| Anionic | SAX; NH2; Polymeric SAX & WAX | ||
| Fluorinated Compounds | Fluoro | ||
| Peptides | Desalting | All | C4; C8; C18; Carbon Black; HILIC |
| SDS removal | All | SDS Removal | |
| Enrichment | Glycopeptide | Carbon Black; HILIC; TiO2 | |
| Phosphopeptide | TiO2; ZrO2; TiO2/ZrO2; SAX; NH2; Polymeric SAX & WAX | ||
| Other peptide | SAX; NH2; SCX; WCX; Polymeric SAX, WAX, SCX & WCX | ||
| Proteins | SDS removal | All | SDS Removal |
| Tryptic digestion | All | Trypsin | |
| Enrichment | Phosphoprotein | TiO2 ; ZrO2; TiO2 / ZrO2; SAX; NH2; Polymeric SAX & WAX | |
| Other protein | SAX; NH2; SCX; WCX; Polymeric SAX, WAX, SCX & WCX | ||
| Oligo-saccharides | Desalting | All | Carbon Black |
| Enrichment | Sulfated glycan | SAX; XSAX | |
| Sialo-glycan | SAX; XSAX | ||
| Other oligosaccharide | Carbon Black; HILIC; TiO2 |
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