How to choose the proper SiliaQuick QuEChERS kit?
Step 1: For Liquid Extraction
Extract the pesticides or analytes of interest into an organic layer by relying on the perfect combination of salts and organic solvents (usually acetonitrile).
The table below presents the SiliaQuick QuEChERS Liquid Extraction kits specially pre-packed with anhydrous salts and/or sorbents to suit the QuEChERS technique of your choice.
Selection Criteria:
- For base or heat-sensitive analytes, use buffered method.
- With doubt, use buffered method.
BLEND AND WEIGH, then:
| Original Method | Buffered Methods | |
|---|---|---|
| AOAC 2007.01 Method | EN 15662 Method | |
| 10 g Sample | 15 g Sample | 10 g Sample |
| 4 g MgSO4; 1.5 g NaCl | 6 g MgSO4; 1.5 g NaOAc | 4 g MgSO4; 1 g NaCl; 1 g SCTD; 0.5 g SCDS |
| PN: QE-0001-100P (packets only) PN: QE-0001-100K (packets & tubes) |
PN: QE-0002-100P (packets only) PN: QE-0002-100K (packets & tubes) |
PN: QE-0003-100P (packets only) PN: QE-0003-100K (packets & tubes |
MgSO4 = Anhydrous Magnesium Sulfate , NaCl = Sodium Chloride, NaOAc = Sodium Acetate, GCB =Graphitized Carbon Black, SCTD=Sodium Citrate Dibasic Sesquihydrate,SCDS=Sodium Citrate Tribasic Dihydrate
Step 2: For dispersive Solid-Phase Extraction Clean-Up
An aliquot of the organic layer from the 1st step is subjected to further clean-up. This step helps selectively remove unwanted interferences such as lipids and pigments but NOT your analytes of interest!
The following table presents the 4 types of SiliaQuick QuEChERS dispersive Solid-Phase Extraction clean-up kits to match your food matrices.
dSPE kits contain pre-weighed sorbents/salts inside 2 mL or 15 mL centrifuge tubes. We recommend using 2 mL dispersive tubes for an extract volume of 1 mL and 15 mL dispersive tubes for extract volumes higher than 3 mL.
Selection Criteria:
- Aliquot size is specified by the method.
- Kits are created for these specific amounts.
- 4 easy-to-choose typed of food matrices.
- Colour-coded caps that match matrix category for reduced error.
- Two methods depending on molecules to be removed
Check pH and adjust to pH 5 – 5.5
Add internal standards aliquot
SHAKE AND CENTRIFUGE, then:
| Cap Color for 2 mL tubes | Matrix | 2 mL tubes for small extract volumes | 15 mL tubes for large extract volumes | ||
|---|---|---|---|---|---|
| AOAC 2007.01 | EN 15662 | AOAC 2007.01 | EN 15662 | ||
| Clear | General matrices
|
150 mg MgSO4 50 mg PSA PN: QD-1000-2T |
150 mg MgSO4 25 mg PSA PN: QD-1001-2T |
1200 mg MgSO4 400 mg PSA PN: QD-2000-15T |
900 mg MgSO4 150 mg PSA PN: QD-2001-15T |
| Pink | Pigmented matrices
|
150 mg MgSO4 50 mg PSA 50 mg GCB PN: QD-1002-2T |
150 mg MgSO4 25 mg PSA 2.5 mg GCB PN: QD-1003-2T |
1200 mg MgSO4 400 mg PSA 400 mg GCB PN: QD-2002-15T |
900 mg MgSO4 150 mg PSA 15 mg GCB PN: QD-2003-15T |
| Green | Highly pigmented matrices
|
150 mg MgSO4 50 mg PSA 50 mg GCB 50 C18 PN: QD-1004-2T |
150 mg MgSO4 25 mg PSA 7.5 mg GCB PN: QD-1005-2T |
1200 mg MgSO4 400 mg PSA 400 mg GCB 400 mg C18 PN: QD-2004-15T |
900 mg MgSO4 150 mg PSA 45 mg GCB PN: QD-2005-15T |
| Blue | Fatty and waxed matrices
|
150 mg MgSO4 50 mg PSA 50 mg C18 PN: QD-1006-2T |
150 mg MgSO4 25 mg PSA 25 mg C18 PN: QD-1007-2T |
1200 mg MgSO4 400 mg PSA 400 mg C18 PN: QD-2006-15T |
900 mg MgSO4 150 mg PSA 150 mg C18 PN: QD-2007-15T |
Step 3: For dispersive Solid-Phase Extraction Clean-Up
The extract, containing the analyte(s) is ready to be analysed by GC or HPLC with MS, MS/MS or your selective detector.
Selection Criteria:
- Consider your application (analyte MW, solubility).
- Consider the stationary phase (column chemistry, separation mode, particle size, retention capacity).
- Column dimensions (internal diameter, length).
SHAKE AND CENTRIFUGE, then:
| SiliaChrom dt C18 | SiliaChrom XDB C18 | SiliaChrom SCX |
|---|---|---|
| Universal C18 for most popular applications (highest purity silica gel) | Ideal for barbiturates, fat-soluble vitamins, fatty acids, steroids | Ideal for charged analytes |
16 Item(s)
List Grid
-
SiliaQuick™ QuEChERS for General Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 2 mL tube (QD-1000-2T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method,for general fruits & vegetables. 2mL format. -
SiliaQuick™ QuEChERS for General Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 2 mL tube (QD-1001-2T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for general fruits & vegetables. 2mL format. -
SiliaQuick™ QuEChERS for General Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 15 mL tube (QD-2000-15T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method, for general fruits & vegetables. 15mL format. -
SiliaQuick™ QuEChERS for General Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 15 mL tube (QD-2001-15T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for general fruits & vegetables.15mL format. -
SiliaQuick™ QuEChERS for Pigmented Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 2 mL tube (QD-1002-2T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method, for pigmented fruits & vegetables. 2mL format. -
SiliaQuick™ QuEChERS for Pigmented Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 2 mL tube (QD-1003-2T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for pigmented fruits & vegetables. mL format. -
SiliaQuick™ QuEChERS for Pigmented Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 15 mL tube (QD-2002-15T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method, for pigmented fruits & vegetables. 15mL format. -
SiliaQuick™ QuEChERS for Pigmented Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 15 mL tube (QD-2003-15T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for pigmented fruits & vegetables. 15mL format. -
SiliaQuick™ QuEChERS for Highly Pigmented and Fatty Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 2 mL tube (QD-1004-2T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method, for highly pigmented and fatty fruits & vegetables. 2mL format. -
SiliaQuick™ QuEChERS for Highly Pigmented and Fatty Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 2 mL tube (QD-1005-2T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for highly pigmented and fatty fruits & vegetables. 2mL format. -
SiliaQuick™ QuEChERS for Highly Pigmented and Fatty Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 15 mL tube (QD-2004-15T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method, for highly pigmented and fatty fruits & vegetables. 15mL format. -
SiliaQuick™ QuEChERS for Highly Pigmented and Fatty Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 15 mL tube (QD-2005-15T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for highly pigmented and fatty fruits & vegetables. 15mL format. -
SiliaQuick™ QuEChERS for Fatty & Waxed Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 2 mL tube (QD-1006-2T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method, for highly fatty and waxed fruits & vegetables. 2mL format. -
SiliaQuick™ QuEChERS for Fatty & Waxed Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 2 mL tube (QD-1007-2T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for fatty and waxed fruits & vegetables. 2mL format. -
SiliaQuick™ QuEChERS for Fatty & Waxed Fruits & Vegetables Dispersive SPE Clean-up Kit for AOAC 2007.01, 15 mL tube (QD-2006-15T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, AOAC 2007.01 method, for fatty and waxed fruits & vegetables. 15mL format. -
SiliaQuick™ QuEChERS for Fatty & Waxed Fruits & Vegetables Dispersive SPE Clean-up Kit for EN 15662, 15 mL tube (QD-2007-15T)
Dispersive cleaning kit for the SiliaQuick™ QuEChERS, EN 15662 method, for fatty and waxed fruits & vegetables. 15mL format.
16 Item(s)
List Grid
