How to choose the proper SiliaQuick QuEChERS kit?

Step 1: For Liquid Extraction

Extract the pesticides or analytes of interest into an organic layer by relying on the perfect combination of salts and organic solvents (usually acetonitrile).

The table below presents the SiliaQuick QuEChERS Liquid Extraction kits specially pre-packed with anhydrous salts and/or sorbents to suit the QuEChERS technique of your choice.

Selection Criteria:

• For base or heat-sensitive analytes, use buffered method.
• With doubt, use buffered method.

BLEND AND WEIGH, then:

MgSO₄ = Anhydrous Magnesium Sulfate , NaCl = Sodium Chloride, NaOAc = Sodium Acetate, GCB =Graphitized Carbon Black, SCTD=Sodium Citrate Dibasic Sesquihydrate,SCDS=Sodium Citrate Tribasic Dihydrate

Step 2: For dispersive Solid-Phase Extraction Clean-Up

An aliquot of the organic layer from the 1st step is subjected to further clean-up. This step helps selectively remove unwanted interferences such as lipids and pigments but NOT your analytes of interest!

The following table presents the 4 types of SiliaQuick QuEChERS dispersive Solid-Phase Extraction clean-up kits to match your food matrices.

dSPE kits contain pre-weighed sorbents/salts inside 2 mL or 15 mL centrifuge tubes. We recommend using 2 mL dispersive tubes for an extract volume of 1 mL and 15 mL dispersive tubes for extract volumes higher than 3 mL.

Selection Criteria:

• Aliquot size is specified by the method.
• Kits are created for these specific amounts.
• 4 easy-to-choose typed of food matrices.
• Colour-coded caps that match matrix category for reduced error.
• Two methods depending on molecules to be removed

Check pH and adjust to pH 5 – 5.5
Add internal standards aliquot
SHAKE AND CENTRIFUGE, then:

Step 3: For dispersive Solid-Phase Extraction Clean-Up

The extract, containing the analyte(s) is ready to be analysed by GC or HPLC with MS, MS/MS or your selective detector.

Selection Criteria:

• Consider your application (analyte MW, solubility).
• Consider the stationary phase (column chemistry, separation mode, particle size, retention capacity).
• Column dimensions (internal diameter, length).

SHAKE AND CENTRIFUGE, then: