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Aflatoxins

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Matrix

Peanut butter

Analytes

Aflotoxin M1, Aflotoxin G2, Aflotoxin G1, Aflotoxin B2, Aflotoxin B1

SPE Cartridge

SiliaPrep Florisil PR
500 mg / 3 mL
SPE-AUT-0015-03P

SiliaPrep Silica
200 mg / 3 mL
SPE-R10030B-03G

Sample Pretreatment before Loading

Add 40 mL of Methanol / Water (80:20) and 0.2 g of Sodium Chloride to 5 g of peanut butter. Stir for 2 hours, filter on paper and rinse with 15 mL of Methanol. Dry the combined extracts with Magnesium Sulfate and evaporate under Nitrogen. Reconstitute with 500 μL of Methanol / Water (80:20).

Conditionning 1 (Florisil PR)

Better recoveries when no conditionning

Equilibration 1 (Florisil PR)

Better recoveries when no equilibration

Loading 1 (Florisil PR)

Pretreated sample

Washing 1 (Florisil PR)

2 x 3 mL of Methanol / Water (80:20)
2 x 3 mL of Methanol

Elution 1 (Florisil PR)

2 x 3 mL of Acetone / Water / Formic Acid (96:3.5:0.5)

Further Treatment 1 (Florisil PR)

Combine elution fractions, evaporate under Nitrogen and reconstitute with 2 mL of Hexane / Chloroform (50:50).

Conditionning 2 (Silica)

2 x 3 mL of Hexane

Loading 2 (Silica)

Sample extracted from SiliaPrep Florisil PR cartridge

Washing 2 (Silica)

3 x 2 mL of Methanol / Chloroform (50:50).

Further Treatment 2 (Silica)

Combine loading and washing fractions, evaporate under Nitrogen, reconstitute with 0.1 % Formic Acid and Ammonium Acetate (5 mM) in Water / Methanol (50:50) and quantify by LC/MS.

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  1. SiliaPrep SPE Cartridges Silica (SPE-R10030B)

    SiliaPrep SPE Cartridges Silica (SPE-R10030B)

    SiliaPrep Silica is the most polar sorbent presenting a slightly acidic character used to extract various compounds from non-polar solvents using hydrogen bonding. Efficient sorbent for the separation of analytes with similar structures and for removing the baseline noise from organic samples.
  2. SiliaPrep SPE Cartridges Florisil PR Grade, 150 - 250 µm, 100 Å (SPE-AUT-0015)

    SiliaPrep SPE Cartridges Florisil PR Grade, 150 - 250 µm, 100 Å (SPE-AUT-0015)

    SiliaPrep Florisil is a polar sorbent presenting basic character used to extract non-polar to moderate polar compounds from non-polar solvents. This sorbent contain magnesium ion which allows retention for chlorinated pesticides.

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