Aflatoxins

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Matrix

Peanut butter

Analytes

Aflotoxin M1, Aflotoxin G2, Aflotoxin G1, Aflotoxin B2, Aflotoxin B1

SPE Cartridge

SiliaPrep Florisil PR
500 mg / 3 mL
SPE-AUT-0015-03P

SiliaPrep Silica
200 mg / 3 mL
SPE-R10030B-03G

Sample Pretreatment before Loading

Add 40 mL of Methanol / Water (80:20) and 0.2 g of Sodium Chloride to 5 g of peanut butter. Stir for 2 hours, filter on paper and rinse with 15 mL of Methanol. Dry the combined extracts with Magnesium Sulfate and evaporate under Nitrogen. Reconstitute with 500 μL of Methanol / Water (80:20).

Conditionning 1 (Florisil PR)

Better recoveries when no conditionning

Equilibration 1 (Florisil PR)

Better recoveries when no equilibration

Loading 1 (Florisil PR)

Pretreated sample

Washing 1 (Florisil PR)

2 x 3 mL of Methanol / Water (80:20)
2 x 3 mL of Methanol

Elution 1 (Florisil PR)

2 x 3 mL of Acetone / Water / Formic Acid (96:3.5:0.5)

Further Treatment 1 (Florisil PR)

Combine elution fractions, evaporate under Nitrogen and reconstitute with 2 mL of Hexane / Chloroform (50:50).

Conditionning 2 (Silica)

2 x 3 mL of Hexane

Loading 2 (Silica)

Sample extracted from SiliaPrep Florisil PR cartridge

Washing 2 (Silica)

3 x 2 mL of Methanol / Chloroform (50:50).

Further Treatment 2 (Silica)

Combine loading and washing fractions, evaporate under Nitrogen, reconstitute with 0.1 % Formic Acid and Ammonium Acetate (5 mM) in Water / Methanol (50:50) and quantify by LC/MS.